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20204
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Subject: Environmental Science × End date: 2020 × Start date: 2020 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Removal of pollutants present in wastewater by inactive biomass of Pseudomonas sp.
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2020-12) Sati, Prayanjali; Srivastava, R.K.
    The present study was undertaken to assess the pollutant removal efficiency of bioadsorbent from mixed water consisting both domestic and industrial waste water. Bioadsorbent was prepared by dead biomass of Pseudomonas fluorescence.To determine pollutant characteristics initial concentration of various parameters of wastewater were analyzed. The prepared bioadsorbent were used to remove various water pollutants that include BOD, COD, sodium, potassium, chloride and Kjeldahl nitrogen present in wastewater sample. Treatment of wastewater was carried out using different dosages (8g, 12g and 16g)of bioadsorbent prepared by dead biomass of above bacteria in 1 liter of the wastewater sample at 3 different contact times (30, 60 and 90min) and pH (4,6 and 8). Maximum biosorption of pollutants were observed using 16g/l dosage, at 6 pH with 90 min contact time. For evaluating removal efficiency of bioadsorbent it was used for further second and third cycles which concludes that by increasing cycles its removal efficiency get reduced. For regeneration of bioadsorbent NaOH was used which make it efficient and ecofriendly option for wastewater treatment having higher efficiency to remove pollutants in very less contact time. For analyzing removal efficiencies of various pollutants levels using bioadsorbent was also studied through Langmuir and Freundlich adsorption isotherm models. In the present study Freundlich adsorption isotherm model was found to be more applicable and favorable. The removal efficiencies of various pollutant levels were analyzed statistically by using regression analysis. Based on results obtained, it is noted that dry biomass of Pseudomonas fluorescens as a bacterial bioadsorbent would be a good option for the removal of pollutants present in wastewater consisting both domestic and industrial.
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    ThesisItemOpen Access
    Immobilization of microbial cells: A promising tool for wastewater treatment
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2020-12) Alisha Rani; Srivastava, R.K.
    The present study was done in order to evaluate the potential of suspended and immobilized bacterial cells of Bacillus cereus for the removal of water pollutants from domestic wastewater. Initial parameters of domestic wastewater were analysed to determine the pollutants level in wastewater. Immobilization of above bacteria was carried out with the help of sodium alginate beads. The prepared cell beads and suspended cell culture were used to remove water pollutants present in the wastewater sample, that include BOD, COD, sodium, potassium, nitrite, fluoride, sulphate, phosphate, TDS and arsenic. All treatments were performed in flasks with 150 ml of wastewater at three different retention times (2 hours, 4 hours and 6 hours). Maximum removal efficiency was recorded using immobilized Bacillus cereus beads at 6 hours retention time. By various treatments given, the pollutant concentration of domestic wastewater was reduced and brought it under to meet the permissible limits for discharge. Based on the results obtained it was found that the use of immobilized microbial cells is a cost effective and efficient method for the removal of water pollutants from domestic wastewater.
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    ThesisItemOpen Access
    Effect of heavy metals (Nickel and Chromium) on the development of root zone bacteria of Pisum sativum
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2020-11) Pandey, Diksha; Rai, J.P.N.
    The present study focuses on characterization of Rhizobium colonies obtained from root nodules of Pisum sativum plant and to investigate their tolerance efficiency towards heavy metals Nickel and Chromium based on CFU count, nodule number, weight, plant height and leghaemoglobin content. The result indicated that a bacterial colony isolated from root nodules of pea on YЕMA medium are milky white colony with spherical convex surface. Biochemical tests of the isolated colonies like oxidase, catalase and bromothymol blue were positive, while starch hydrolysis, lipase test, lysine decarboxylase and caseinase were negative which reveal that the colonies isolated frompea plant belongs to Rhizobia species. The presence of high concentration of Nickel and Chromium led to reduction in rhizobial colony. The study indicated that all the colonies grew well at pH 6 and 7, temperature 28º C to 30º C and at salt concentration of 1%. Pisum sativum also work as a remediator. Heavy metal analysis using Atomic Absorption Spectrophotometer in roots reveals that Cr concentrations were found higher (p<0.05) when grown in treated soil than Nickel treated soil. Nickel and Chromium were found to be the more phytotoxic and significantly (p<0.05) reduced the leghaemoglobin content in all the metal treatments and decreased the leghaemoglobin at all the three concentrations among all the doses.
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    ThesisItemOpen Access
    Assessment of rhizofiltration of lead and cadmium using Chrysopogon zizanioides
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2020-08) Prabakaran, M.; Saraswat, Shweta
    The present study focused on rhizofiltration potential of Chrysopogon zizanioides for removal of lead and cadmium from synthetic waste water. Accumulation of metals in plant parts and their simultaneous removal from synthetic waste water were analysed. Pb and Cd content in waste water was decreasing with increasing exposure duration of the vetiver. Pb and Cd accumulation in plant was increasing with increasing exposure duration. Higher Pb and Cd removal (i.e. 13.04 ppm and 3.9 ppm respectively) was observed in 50 ppm than 100 ppm concentration of waste water at 5th week. Maximum Pb reduction rate i.e. 0.541 mg/L/day at 5th week was recorded, while Cd reduction rate i.e. 0.186 mg/L/day at 4th week was observed in 50 ppm concentrated waste water. Metal removal efficiency of vetiver grass increased with increasing exposure period. Plant had the highest Pb and Cd removal efficiency i.e. 26.06% and 7.79% respectively at 50 ppm. The plant had higher Pb and Cd accumulation at 50 ppm than 100 ppm of waste water. Maximum Pb and Cd accumulation (i.e. 1.981 mg g-1 and 0.795 mg g-1 respectively) was observed in roots at 50 ppm. However, in shoots maximum Pb and Cd content was (i.e. 0.077 mg g-1 and 0.077 mg g-1) respectively at 50 ppm. Total Pb and Cd accumulation in plant was reached up to 38.48 mg/plant and 9.53 mg/plant at 50 ppm. Dry biomass production of vetiver grass increased with decreasing Pb and Cd concentration in waste water. Result revealed that vetiver plant having good metal removal efficiency could better be employed for decontaminating moderately polluted waste water.