Effect of heavy metals (Nickel and Chromium) on the development of root zone bacteria of Pisum sativum
Loading...
Date
2020-11
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
The present study focuses on characterization of Rhizobium colonies obtained from root nodules of Pisum sativum plant and to investigate their tolerance efficiency towards heavy metals Nickel and Chromium based on CFU count, nodule number, weight, plant height and leghaemoglobin content. The result indicated that a bacterial colony isolated from root nodules of pea on YЕMA medium are milky white colony with spherical convex surface. Biochemical tests of the isolated colonies like oxidase, catalase and bromothymol blue were positive, while starch hydrolysis, lipase test, lysine decarboxylase and caseinase were negative which reveal that the colonies isolated frompea plant belongs to Rhizobia species. The presence of high concentration of Nickel and Chromium led to reduction in rhizobial colony. The study indicated that all the colonies grew well at pH 6 and 7, temperature 28º C to 30º C and at salt concentration of 1%. Pisum sativum also work as a remediator. Heavy metal analysis using Atomic Absorption Spectrophotometer in roots reveals that Cr concentrations were found higher (p<0.05) when grown in treated soil than Nickel treated soil. Nickel and Chromium were found to be the more phytotoxic and significantly (p<0.05) reduced the leghaemoglobin content in all the metal treatments and decreased the leghaemoglobin at all the three concentrations among all the doses.