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ThesisItem Open Access Biochemical and pharmacological profiling of different genotypes of fenugreek (Methi)(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2013-07) Belal, Babita; Viveka NandIn the present study seeds of fenugreek plant were collected from different altitudes of Kumaun region of Uttarakhand. 14 fenugreek genotypes collected were analysed for their total phenolic, flavonoid, ortho dihydroxy phenolic, ascorbic acid, carbohydrate content, antioxidant activity, antifungal activity, antidiabetic activity and elemental profile. Genotype PM-14 exhibited maximum DPPH scavenging activity (67.77 - 91.94%), PM-18 had the maximum FRAP value (35.45mg/100mg) while chelating activity was found maximum in PM-14 (87.86%), superoxide scavenging activity was maximum in PEB (65.85%) and total antioxidant activity for all the genotypes as ascorbic acid equivalent ranges from 0.15-0.22mg/100mg. Phenolic content ranged from 1.92-4.6mg/100mg, ortho dihydroxy phenols (0.34-0.59mg/100mg), flavonoid (1.08-3.06mg/100mg), ascorbic acid (0.80-1.41 mg/gm) in methanolic seed extract of all genotypes. Maximum ascorbic acid content was found in PM(C)-1 (1.41mg/gm) while minimum was in Hisar sonali (0.80 mg/gm). Highest total sugar were in PM-15 (9.15mg/100mg), maximum reducing sugar in PM-14 (1.21 mg/100mg) and non-reducing sugar in PM-15 (8.66 mg/100mg). Amount of tannic acid ranged from from 0.42-0.87mg/100mg and proenthrocynidines from 2.73-5.86mg/100mg. IC50 value for α-amylase and α- glucosidase enzyme inhibition assays in PM(C)-1(10.53) and PM 15(29.39) showed maximum inhibition. All samples showed a good antifungal activity against phytopathogenic fungi Rhizoctonia solani, Sclerotium rolfsii and Colletotricum gleostorides. Elemental analysis by Atomic Absorption Spectroscopy revealed that the iron content was maximum in all the fenugreek genotypes.ThesisItem Open Access Phytochemical analysis and biological activity determination of Zingiber roseum Rosc(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2016-07) Arya, Sureshkumar Premram; Om PrakashZingiber roseum was collected from Padampuri, Uttarakhand in the month of September-October, 2015. The essential oil was isolated from fresh seeds by hydrodistillation using Clevenger’s type apparatus. Essential oil and all extracts were analysed by GC-MS for their phytochemical composition. Extracts of seeds, rhizome and perianth obtained in organic solvent of different polarity were for their antifungal activity against two phytopathogenic fungi viz; Rhizoctonia solani and Fusarium accuminata, antioxidant assay by different methods viz; reducing power activity, chelating activity of Fe2+, DPPH radical scavenging activities. All the methanolic extracts were studied for their qualitative analysis of phenolics by HPLC and herbicidal activity on Raphanus sativus (radish). The dried and powdered seeds, rhizomes and perianth were studied for their mineral profiling with help of Atomic Absorption Spectroscopy (AAS). The GC and GC-MS analysis revealed the presence of β–cubene (13.4%), caryophyllene (12.6%) and neryl linalool isomer (24.06%) were identified as major compounds compounds in oil. However neryl linalool isomer, geranyl linalool isomer, β-citronellal, 9-octadecenoic acid, l-(+)-ascorbic acid 2,6dihexadecanoate, 2-(4-hydroxy-3-methoxyphenyl)-3,7-dimethoxy-4h-chromen-4-one, 9,12-octadecadienoic acid (Z,Z)-, methyl ester and hexadecanoic acid, methyl ester were found major compounds in different extracts. HPLC analysis revealed the presence of ferullic acid, gallic acid and vanillic acid in different parts of plant. Extracts exhibited good to moderate antioxidant activity in dose dependent manner. Among all the extracts, Z. roseum perianth methanolic extract (ZRPME) possessed maximum DPPH radical scavenging activity; Z. roseum seed methanolic extract (ZRSME) showed maximum reducing power activity and maximum metal chelating activity was shown by Z. roseum perianth hexane extract (ZRPHE). Quantative analysis of total phenols, orthodihydric phenols and flavonoids ranged from (0.16±0.0 to 4.5±0.0 mg/g GAE), (0.01±0.0 to 4.4±0.0 mg/g CLE) and (11.5±0.0 to 78.2±0.01 mg/g CNE) in different extracts respectively. All extracts were found to be active against both fungi. All methanolic extracts inhibited the fungal mycelial growth of F. accuminata, while the extracts also possessed fungal growth promoting against R. solani. Z. roseum seed methanolic extract (ZRSME) was found most effective for inhibiting the growth of fungus. The extracts inhibited and promoted mycelial growth in respective fungi in dose dependent manner. The methanolic extract of Z. roseum were found to be active and inhibited the germination in the seeds of R. sativus (radish) and Z. roseum rhizome methanolic extract ZRRME showed more seed germinating activity than Z. roseum seed methanolic extract (ZRSME). Elemental analysis of various parts revealed the presence of Fe, Mn, Zn, Cu, Mg, Cd, Cr, N, P and K in varying quantities. Based on the above results it can be concluded that Zingiber roseum can be a good source of natural antioxidant, anti-fungal and herbicide after proper clinical trials besides its academic importance.ThesisItem Open Access Persistence of Imidacloprid insecticide in okra and its harvest time residue in paddy crop and soil of tarai region(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2016-06) Dhiman, Megha; Srivastava, AnjanaThesisItem Open Access Square wave voltammetric detection of isoproturon at nanocomposite electrode(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2016-07) Bhatt, Priya; Zaidi, M.G.H.ThesisItem Open Access Synthesis and biological activity of Coumarin and Spirooxindole derivatives(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2016-07) Singh, Pooja; Singh, VirendraThesisItem Open Access Studies on electrical and thermal characteristics of polypyrrole/haemoglobin composites(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2016-07) Rana, Nivedita; Sand, N.K.