Biochemical and pharmacological profiling of different genotypes of fenugreek (Methi)
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Date
2013-07
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
In the present study seeds of fenugreek plant were collected from different altitudes of Kumaun region of Uttarakhand. 14 fenugreek genotypes collected were analysed for their total phenolic, flavonoid, ortho dihydroxy phenolic, ascorbic acid, carbohydrate content, antioxidant activity, antifungal activity, antidiabetic activity and elemental profile. Genotype PM-14 exhibited maximum DPPH scavenging activity (67.77 - 91.94%), PM-18 had the maximum FRAP value (35.45mg/100mg) while chelating activity was found maximum in PM-14 (87.86%), superoxide scavenging activity was maximum in PEB (65.85%) and total antioxidant activity for all the genotypes as ascorbic acid equivalent ranges from 0.15-0.22mg/100mg. Phenolic content ranged from 1.92-4.6mg/100mg, ortho dihydroxy phenols (0.34-0.59mg/100mg), flavonoid (1.08-3.06mg/100mg), ascorbic acid (0.80-1.41 mg/gm) in methanolic seed extract of all genotypes. Maximum ascorbic acid content was found in PM(C)-1 (1.41mg/gm) while minimum was in Hisar sonali (0.80 mg/gm). Highest total sugar were in PM-15 (9.15mg/100mg), maximum reducing sugar in PM-14 (1.21 mg/100mg) and non-reducing sugar in PM-15 (8.66 mg/100mg). Amount of tannic acid ranged from from 0.42-0.87mg/100mg and proenthrocynidines from 2.73-5.86mg/100mg. IC50 value for α-amylase and α- glucosidase enzyme inhibition assays in PM(C)-1(10.53) and PM 15(29.39) showed maximum inhibition. All samples showed a good antifungal activity
against phytopathogenic fungi Rhizoctonia solani, Sclerotium rolfsii and Colletotricum gleostorides. Elemental analysis by Atomic Absorption Spectroscopy revealed that the iron content was maximum in all the fenugreek genotypes.
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