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20131
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Subject: Biotechnology × Author: Lovepreet Singh × Type: Thesis × Thesis Type: M.Sc ×
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    GENETIC DIVERSITY AMONG Gossypium arboreum L. GERMPLASM LINES AS DETERMINED BY SSR MARKERS AND MORPHOLOGICAL CHARACTERISTICS
    (Punjab Agricultural University, Ludhiana –, 2013) Lovepreet Singh; Pathak, Dharminder
    The present study was undertaken to study the genetic diversity among Gossypium arboreum germplasm lines using molecular and morphological markers. Sixty four germplasm lines from diverse eco-geographical regions of India were taken for evaluation. Forty SSR primer pairs belonging 11 of the 13 A-genome chromosomes were employed to assess diversity at the molecular level. LD 694 and RAC 024 were observed to be quite diverse from each other. G. hirsutum accession TM1 and G. herbaceum accession RAHS 14 formed separate clusters. PIC value of the markers ranged from 0.11 to 0.79. The dissimilarity coefficient for G. arboreum ranged from 0.08 to 0.29. Mahalanobis D2 distances statistics was used to cluster the genotypes into six groups. Cluster I was the largest with thirty three genotypes followed by cluster II which consisted of 25 genotypes. Cluster III and IV both included two genotypes, whereas, cluster V and VI were having one genotype each. The maximum inter-cluster distance was 97.85 (cluster IV & V). The maximum intra-cluster distance was 30.68 (cluster I). Both the estimates of genetic distances did not find any relationship between genetic diversity and eco-geographical distribution. Several morphological characters such as plant body colour, flower colour, leaf shape, presence/ absence of leaf nectar did not have any impact on the clustering pattern of the genotypes