GENETIC DIVERSITY AMONG Gossypium arboreum L. GERMPLASM LINES AS DETERMINED BY SSR MARKERS AND MORPHOLOGICAL CHARACTERISTICS
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Date
2013
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Punjab Agricultural University, Ludhiana –
Abstract
The present study was undertaken to study the genetic diversity among Gossypium
arboreum germplasm lines using molecular and morphological markers. Sixty four
germplasm lines from diverse eco-geographical regions of India were taken for evaluation.
Forty SSR primer pairs belonging 11 of the 13 A-genome chromosomes were employed to
assess diversity at the molecular level. LD 694 and RAC 024 were observed to be quite
diverse from each other. G. hirsutum accession TM1 and G. herbaceum accession RAHS 14
formed separate clusters. PIC value of the markers ranged from 0.11 to 0.79. The dissimilarity
coefficient for G. arboreum ranged from 0.08 to 0.29. Mahalanobis D2 distances statistics was
used to cluster the genotypes into six groups. Cluster I was the largest with thirty three
genotypes followed by cluster II which consisted of 25 genotypes. Cluster III and IV both
included two genotypes, whereas, cluster V and VI were having one genotype each. The
maximum inter-cluster distance was 97.85 (cluster IV & V). The maximum intra-cluster
distance was 30.68 (cluster I). Both the estimates of genetic distances did not find any
relationship between genetic diversity and eco-geographical distribution. Several
morphological characters such as plant body colour, flower colour, leaf shape, presence/
absence of leaf nectar did not have any impact on the clustering pattern of the genotypes
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