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2001 - 20097
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Subject: Biochemistry × End date: 2009 × Start date: 2001 × Type: Thesis ×
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Now showing 1 - 7 of 7
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    ThesisItemOpen Access
    A Study of the Toxicity of Nitrosamines under hypercholesterolemic Conditions and Role of Dietary Fibre
    (Department of Biochemistry College of Basic Sciecnes & Humanities, PAU-Ludhiana, 2001) Mittal, Gaurav
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    ThesisItemOpen Access
    Effect of fibre digesting enzymes on in vitro and in sacco degradation of various feedstuffs in buffalo calves
    (Department of Biochemsitry, College of Basic Sciences and Humanities PAU, Ludhiana, 2001) Chopra, Smily; Sareen, V. K
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    Identification of drought tolerance related biochemical indices in a wheat recombinant population
    (Punjab Agricultural University, Ludhiana, 2009) Gulati, Ankit; Asthir, Bavita
    The present research was aimed at evaluation of biochemical indices of drought tolerance in wheat. Root and shoot tissue of six wheat genotypes viz.C306, C273, PBW550, PBW534, PBW175 and PBW343 were evaluated at 6 day post germination for growth parameters, relative water content (RWC), membrane stability related parameters, enzyme of carbohydrate metabolism, carbohydrate content and osmolytes accumulation under drought stress (simulated using 8% polyethylene glycol). Stress causes short stature in shoot and elongated roots in all genotypes. C306, C273, PBW534 and PBW175 were found to have longest roots under drought stress. C273, C306 and PBW343 maintained higher RWC under stress. Lowest membrane injury index (MII) and thiobarbituric acid reactive substances (TBARS) were found in C273 and PBW343. Lowest decrease in chlorophyll content (about 21%) was found in PBW175 and PBW343 under stress. The activity of sucrolytic enzymes (acid and neutral invertase) and sucrose synthase (cleavage) decreased whereas sucrose synthesizing enzymes (sucrose phosphate synthase and sucrose synthase synthesis) increased under stress in all the genotypes. Lowest sufficient sucrolytic activity was found in C273 and PBW343 whereas C306 and PBW343 maintained higher activity of sucrose synthesizing enzymes. Amylase activity decreased under stress in endosperm to have lower mobilization of starch. PBW175 and C273 were found to have highest sucrose content showing greater osmotic adjustment. Protein content decreased in contrast to amino acid under stress. C273 was found to have highest proline and amino acid content under stress. To establish cause and effect relationship between biochemical traits and drought tolerance a large randomly derived backcross recombinant inbred line (BCRIL) population derived from (C273/PBW343//PBW343) was studied at vegetative stage. Recombinant lines showing drought tolerance in the field showed an average lower proline content compared to sensitive lines. BCRIL population with low MII were drought sensitive. Drought tolerance however, showed a positive association with membrane stability. The other indices (TBARS, RWC and chlorophyll content) were not related to drought tolerance in field.
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    ThesisItemOpen Access
    Molecular Characterisation Of Pasteurella Multocida Isolates From Haemorrhagic Septicaemia In Cattle And Buffaloes
    (Punjab Agricultural University ;Ludhiana, 2001) Arora, Anil Kumar; Jand, S. K.
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    ThesisItemOpen Access
    Studies On Lipid Biosynthesis As Influenced By Position Of Seed On Soybean Stem Axis
    (Punjab Agricultural University; Ludhiana, 2006) Guleria, Shaileja; Munshi, S.K
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    Effect of elicitors on phenolics and related enzymes in brassica juncea l. against alternaria blight
    (Punjab Agricultural University, Ludhiana, 2007) Sood, Neerja; Sohal, B.S.
    Effect of elicitors (Chitosan and Arachidonic Acid) on the induction of resistance against Alternaria blight in Brassica juncea L. was studied. Enzymes of defense system viz. Peroxidase (PO) Superoxide Dismutase (SOD) and Phenylalanine Ammonia Lyase (PAL) and Phenolics (Total phenols, o-dihydroxyphenols and flavanols) were analyzed in germinating seeds and leaves of Brassica juncea L. (var. RLM 619) with elicitor treatment and Alternaria brassicae inoculation. In germination studies, Chitosan and AA at 0.5ppm and 1ppm proved to be good elicitors in eliciting enzyme and phenolics in leaves whereas a little elicitation was observed in plumule and radicle of germinating seeds. In pot house studies, elicitors were applied as foliar spray. 1 and 5 ppm concentrations of chitosan and 10 and 50ppm of AA were sprayed on Brassica juncea L. leaves. Chitosan (5ppm) and AA (10ppm) showed better effect in eliciting defense enzymes and phenolics in the leaves of Brassica juncea L. Increase in defense enzyme coincided with the increase in phenolic content indicating that defense enzyme like PO and PAL are involved in synthesis of phenols, which are involved in lignin-biosynthesis. The studies revealed that foliar spray of chitosan and AA was found to be a better treatment to immunize Brassica juncea L. plants than seed soaking/dressing method.