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20121
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Subject: Biochemistry × Author: Manpreet Kaur × End date: 2019 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    ANALYSIS OF ANTIOXIDANT METABOLISM IN WHEAT SEEDLINGS EXPOSED TO WATER STRESS AND UNDER THE INFLUENCE OF EXOGENOUS ABSCISSIC ACID AND REACTIVE OXYGEN SPECIES
    (Punjab Agricultural University, Ludhiana, 2012) Manpreet Kaur
    The present work was aimed to study the involvement of superoxide anions and ABA biosynthesis in germination, growth and antioxidant response under water stress (WS) and under ABA (A) supply in two wheat cultivars C306 (ABA higher sensitive-cum-drought tolerant) and PBW343 (ABA lesser sensitive-cum-drought susceptible) by using tiron (T) as superoxide scavenger and sodium tungstate (ST) as inhibitor of ABA biosynthesis. Biomass and ascorbate were decreased on scavenging superoxide ions under A in both cultivars. Antioxidant enzymes, H2O2 were decreased in PBW343 but increased in C306 on scavenging superoxide ions under A. ABA inhibitions of shoot length, root length and germination were higher in C306 than in PBW343 and these inhibitions were relieved significantly on scavenging superoxide ions in C306 not in PBW343. Fresh biomasses, antioxidant enzymes, ascorbate/dehydroascorbate ratios were decreased and dehydroascorbate and malondialdehyde contents were increased on inhibiting ABA biosynthesis and on scavenging superoxide anions under water stress in both cultivars but such changes were more significant in C306 than in PBW343. Both cultivars were also compared under H2O2 (H) supply for antioxidant response and for the involvement of ABA biosynthesis under H supply. H2O2 induced antioxidant levels were higher in C306 than in PBW343. On inhibiting ABA biosynthesis under H supply, these H2O2-mediated effects were affected but not completely removed in both cultivars.