ANALYSIS OF ANTIOXIDANT METABOLISM IN WHEAT SEEDLINGS EXPOSED TO WATER STRESS AND UNDER THE INFLUENCE OF EXOGENOUS ABSCISSIC ACID AND REACTIVE OXYGEN SPECIES
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Date
2012
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Punjab Agricultural University, Ludhiana
Abstract
The present work was aimed to study the involvement of superoxide anions and ABA biosynthesis in
germination, growth and antioxidant response under water stress (WS) and under ABA (A) supply in
two wheat cultivars C306 (ABA higher sensitive-cum-drought tolerant) and PBW343 (ABA lesser
sensitive-cum-drought susceptible) by using tiron (T) as superoxide scavenger and sodium tungstate
(ST) as inhibitor of ABA biosynthesis. Biomass and ascorbate were decreased on scavenging
superoxide ions under A in both cultivars. Antioxidant enzymes, H2O2 were decreased in PBW343
but increased in C306 on scavenging superoxide ions under A. ABA inhibitions of shoot length, root
length and germination were higher in C306 than in PBW343 and these inhibitions were relieved
significantly on scavenging superoxide ions in C306 not in PBW343. Fresh biomasses, antioxidant
enzymes, ascorbate/dehydroascorbate ratios were decreased and dehydroascorbate and
malondialdehyde contents were increased on inhibiting ABA biosynthesis and on scavenging
superoxide anions under water stress in both cultivars but such changes were more significant in
C306 than in PBW343. Both cultivars were also compared under H2O2 (H) supply for antioxidant
response and for the involvement of ABA biosynthesis under H supply. H2O2 induced antioxidant
levels were higher in C306 than in PBW343. On inhibiting ABA biosynthesis under H supply, these
H2O2-mediated effects were affected but not completely removed in both cultivars.
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