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2010 - 201118
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End date: 2011 × Start date: 2010 × Thesis Type: M.V.Sc. ×
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Now showing 1 - 9 of 18
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    ThesisItemOpen Access
    INDIVIDUAL AND COMBINED EFFECTS OF FUMONISIN B1 AND OCHRATOXIN A ON THE LIVER OF JAPANESE QUAIL
    (CSKHPKV, Palampur, 2010-07-14) Thakur, Priyanka; Gupta, Vipin K.
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    ThesisItemOpen Access
    INDIVIDUAL AND COMBINED EFFECTS OF FUMONISIN B1 AND OCHRATOXIN A ON THE KIDNEYS OF JAPANESE QUAIL
    (CSKHPKV, Palampur, 2010) Khan, Manzoor Ahmad; Asrani, R.K.
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    ThesisItemOpen Access
    MOLECULAR CHARACTERIZATION OF DIFFERENT CHLAMYDIAE ASSOCIATED WITH VARIOUS INFECTIONS OF RUMINANTS
    (CSKHPKV, Palampur, 2011-07) Bhardwaj, Brijesh; Chahota, Rajesh
    Chlamydial infections have been reported previously among domestic ruminants from various disorders affecting reproductive system, respiratory system and digestive system in Himachal Pradesh, but the molecular characterization of various species/strains of chlamydiae involved has not been done yet, hence we conducted a molecular epidemiological investigation to detect the prevalence of different chlamydial species/strains in domestic ruminants in different parts of state, migratory tracts and in organized farms of Himachal Pradesh. To know the overall chlamydial prevalence in different disease conditions, total 404 samples were screened using Chlamydiales order specific primers based on 23S rRNA and family Chlamydiaceae specific primers based on VD2 region of ompA gene. Result of chlamydial screening showed that 93 (23%) samples, out of 404 samples were positive for chlamydiae, which showed the higher involvement of chlamydiae in various disease conditions. Out of 205 samples of reproductive disorders, 55 (26.82%) samples were showed chlamydial involvement. Among the cases of reproductive disorders, highest i.e. 38 per cent chlamydial association was detected in endometritis followed by 23 per cent in abortions. Out of 147 samples of pneumonia, 23 (15.64%) samples were found positive for chlamydial infection and from 39 samples of enteritis, chlamydial involvement was found in 33.33 per cent samples. Whereas, two samples of conjunctivitis were also found positive for chlamydial involvement. Molecular characterization and genetic variability studies of chlamydial species/strains detected among ruminants by PCR were done either by PCR-RFLP or by study of nucleotide sequence variation of ompA gene in VD2 region. Overall 57 PCR positive samples were characterized and out of which 39 (68.4%) samples were found C. psittaci, 16 (28%) samples were C. abortus, and two (3.5%) samples were found C. pecorum. From PCR positive samples, isolation of chlamydial strains was also attempted using 6 to 8 day old embryonated chicken eggs and isolation of chlamydiae up to 40.74 per cent could be achieved. Besides chlamydiae, involvement of other bacterial agents was also tested in samples from reproductive diseases. Various bacterial isolates like Brucella melitensis 2 (1.1%), Staphylococcus spp. 48 (26.37%), Streptococcus spp. 7 (3.84%), E. coli 47 (25.82%), Bacillus spp. 31 (17.03%), Klebsiella spp. 11 (6.04%), Arcanobacterium spp. 18 (10%), Pseudomonas spp. 13 (7.14%) and other bacterial species. 24 (13.19%) were isolated from different female reproductive disorders of the livestock. The results showed high prevalence of chlamydial infection among ruminants and involvement of multiple chlamydial species was detected in this study.
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    ThesisItemOpen Access
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    ThesisItemOpen Access
    Study on reproductive health status of abandoned cows in Himachal Pradesh
    (CSKHPKV, Palampur, 2010) Chauhan, Akshay Kumar; Singh, Madhumeet;
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    ThesisItemOpen Access
    STUDIES ON GENETIC AND NON GENETIC VARIATIONS OF ANGORA RABBITS REARED IN KANGRA VALLEY OF HIMACHAL PRADESH
    (CSKHPKV, Palampur, 2010-07) SHARMA, Loopamudra; Katoch, Sanjeet
    ABSTRACT This study was conducted to evaluate the phenotypic profiles, genetic and non genetic effects on wool production and reproduction traits in 588 and 361 German Angora rabbits maintained at Angora Rabbit Breeding Farm, Kandwari, Kangra (Himachal Pradesh) from 2000 to 2009 for reproduction traits and from 2000 to 2007 for wool production traits. The data was analyzed by Harvey’s least squares method of fitting constants. The overall least squares means were 4.62 1.64 k, 30.39 0.58 d, 58.77 35.10, 169.77 30.38, 181.00 21.30, 184.73 23.49, 594.22 67.13, 591.82 193.67 and 1185.96 202.96 g respectively for litter size at birth, gestation period, wool yield at first, second, third, fourth clip, first year, second year and cumulative yield. Year and season of kindling significantly affected litter size at birth, wool yield at first, third, fourth clip and first year yield. Gestation period, wool yield at second clip, second year and cumulative yield were affected by year. Heritability estimates were 0.09, 0.29 0.030, 0, 0.312 0.021, 0.27 0.017, 0.412 0.028 and 0.116 0.013 respectively for wool yield at first, second, third, fourth clip, first year, second year and cumulative yield. Genetic correlations of wool yield at second clip with second year and cumulative yield, wool yield at fourth clip with second year and cumulative yield and first year with second year and cumulative yield were negative. Wool yield at first clip with cumulative yield, second with third clip and third with fourth clip, first year, second year and cumulative yield were zero. Wool yield at second clip with fourth clip had the highest genetic correlation (1.226 0.398). Phenotypic correlations of second clip with second year and third clip with second year yield were negative. Second year and cumulative yield had the highest significant phenotypic correlation (0.942). Winter appeared to be the most favorable season for breeding of Angora rabbits. Improvement can be achieved with proper selection and improved management practices. Pure breeding stock of Angora rabbits can be introduced to maintain superior genotypes and variability.
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    ThesisItemOpen Access
    INVESTIGATIONS ON HEPATIC DYSFUNCTIONS IN CANINE
    (CSKHPKV, Palampur, 2010-09-13) SHARMA, Saurabh; Wadhwa, Des Raj
    The study was conducted on 894 dogs presented at Veterinary Clinical Complex CSKHPKV, Palampur (H.P.). On the basis of history and clinical examination, dogs suspected for hepatic dysfunction were subjected to haemato-biochemical examinations, radiography and ultrasonography to confirm hepatic diseases. Among these, 26 dogs (2.91%) dogs with different hepatic dysfunctions were diagnosed, out of which 80.77% were of primary liver disorders and 19.23% were of reactive hepatopathies. Among primary hepatopathies, the acute hepatitis formed the largest group (57.69%) followed by cirrhosis (19.23%) and hepatitis with jaundice (15.38%) and a single case (3.85%) of hepatic tumour. The mean age of dogs having primary hepatic dysfunctions was higher (53.09 ± 9.23 months) than the dogs having reactive hepatopathies (23.80 ± 7.94 months). The dogs suffering from liver dysfunctions showed symptoms like inappetance/anorexia, ascites, vomition, dullness, constipation, fever, diarrhoea, pale mucosa, jaundice, weight loss, melaena and weakness. Haematological examination revealed significantly decreased haemoglobin and packed cell volume in primary hepatic dysfunctions and neutrophilic leucocytosis in hepatitis and reactive hepatopathy. The mean activities of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatse were markedly higher in all the hepatic dysfunctions. Biochemical examination revealed hypoproteinaemia and hypoalbuminaemia in primary hepatic dysfunctions. Ultrasonographic studies were found very helpful in evaluation of liver status and classification of hepatopathies. Following the combined therapy comprising hepatoprotectant (Silymarin), fluid therapy, liver extract, antibiotic, antiemetic and diuretics, 60 per cent cases of hepatitis and 40 percent cases of cirrhosis could be treated successfully.
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    ThesisItemOpen Access
    CLINICO-THERAPEUTIC STUDIES ON BOVINE CUTANEOUS PAPILLOMATOSIS
    (CSKHPKV, Palampur, 2010-12) Rana, Sanjeev; Wadhwa, Des Raj
    The present investigation was undertaken to carry out epidemiological, clinical and therapeutic studies on bovine cutaneous papillomatosis. Epidemiological survey at various dairy farms comprising 843 cattle revealed papillomatosis in 6.16 per cent. The warts were more frequent in the age above 3 years (68.04%). The incidence was higher in cross bred animals and Jersey cross was maximum affected (74.22%). All the affected animals were females. Mostly heifers (29.89%), 1st lactation (26.80%), and 2nd lactation (21.64%) animals were affected. Teats papillomatosis was recorded in 93.81 per cent cases. Warts on other body parts were present in 17.53 per cent cases, which included warts on muzzle, neck, shoulder, ventral abdomen, legs and inner aspect of thigh. The warts were severe (more than 20) in most animals (55.67%), less than 1 cm (72 %) and mostly of flat type (62.88%). Haematobiochemical profile of affected animals revealed no change than control group. Histopathologically, there was thickening of epidermis, degenerative changes in stratum granulosum and hyperkeratinization of stratum corneum. Combined use of anthiomaline along with levamisole was more efficacious (60 %) than anthiomaline alone (54.54%). Autogenous wart vaccine was effective in 71.42 per cent cases. Levamisole and ivermectin were effective in early and mild cases only and were effective in 27.27 and 36.36 per cent cases respectively. Based on the present study, it is advocated to use combination of anthiomaline and levamisole or autogenous vaccine for treatment of papillomatosis in cattle.
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    ThesisItemOpen Access
    STUDIES ON SOME ASPECTS OF BOVINE MASTITIS IN KANGRA VALLEY OF HIMACHAL PRADESH
    (CSKHPKV, Palampur, 2010-06-22) Kaushik, Manoj; Pal, B.
    Mastitis is the most serious disease of lactating animals especially in high yielders. In India, losses due to mastitis amounting to the tune of rupees 2809.32 crores per annum were reported. The present investigation was carried out for a period of 1 year and 3 months. A total of 60 animals (54 cows and 6 buffaloes) comprising of 98 quarters, suffering from various types of mastitis, were included in this study. Clinical appraisal of mastitic cows and buffaloes; various direct and indirect tests viz., Strip cup test, MCMT, BTB indicator card method, WST and SCC were applied for the diagnosis. The incidence was also recorded. Microbial cultures of milk samples from affected quarters and in vitro drug sensitivity were also done. The status of micronutrients (zinc and copper), Vitamin E and acute phase proteins viz., ceruloplasmin, fibrinogen and albumin were estimated at pre and post- treatment. Treatment was done either with appropriate antibiotic alone or in combination with either tri-sodium citrate or zinc sulphate or vitamin E and selenium as per treatment schedule assigned. The overall incidence of the disease was observed to be 12.83% in College Veterinary Clinics & 6.94% in University Livestock Farm during the study period. On the basis of animals affected, the maximum incidence of mastitis was observed in the sub-acute form (46.67%) followed by acute (18.33%); whereas on quarters basis, the highest incidence was observed in sub-acute (44.90%) followed by acute (18.36%). Highest incidence was observed in crossbred (75.00%) and it was in the 4 th parity (38.33%). High yielders (30.00%) in the early stage of lactation (60.00%) and in rainy season (40.00%) were more prone to mastitis. Besides, left side quarters (60.20%) especially the fore quarters (51.02%) were mainly affected.SCC was significantly higher (p<0.01) in all types of mastitic milk. Staphylococcus spp. (58.06%) was the major isolate followed by Streptococcus spp. (19.36%), E.coli (12.90%), Bacillus spp. (5.38%) and Pseudomonas spp. (4.30%) associated with mastitis. Serum level of ceruloplasmin and plasma level of fibrinogen were found significantly higher (p<0.01) whereas albumin decreased (p<0.01) in mastitic animals and the mean values of these parameters became almost normalized at post-treatment. Zinc concentration was decreased but copper concentration was increased and Vitamin E was also decreased in mastitic animals. These values almost normalized at post-treatment. Clinically, intramammary administration of cefoperazone alongwith parenteral administration of Vit. E & Se was most effective in mastitis.