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20161
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Subject: Veterinary Microbiology × Start date: 2000 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    CELLULAR, IMMUNOLOGICAL AND MOLECULAR RESPONSES AGAINST PASTEURELLA MULTOCIDA IN CALVES
    (CSKHPKV, Palampur, 2016-01-23) Katoch, Shailja; Sharma, Mandeep
    ABSTRACT The present study determined the cellular, molecular and immunological responses following P. multocida infection and vaccination in calves. Four months old nine clinically healthy male cross bred calves were divided into three groups. Calves in vaccinated group were immunized with 2 ml commercial formalin killed alum adjuvant HS vaccine s/c, infected group challenged i/n with 5ml of the inoculum containing 3×109 cfu/ml/nostril of P. multocida B:2 and control group was given 5 ml PBS i/n/nostril. The clinical observations were monitored and serum, nasal secretions and whole blood samples were collected. The mean rectal temperature and mean total clinical score of the infected group was significantly higher than other groups from day 3 to 8 and day 2 to 20, respectively. The serum IgG and IgM response and IgA response in nasal secretions in the infected group was significantly higher than vaccinated group. The CD3+, CD4+, CD8+, CD3+CD4+ and CD3+CD8+ T cells increased in both vaccinated and infected group, however the increase was significant only in infected group. There was decrease in WC1+ and CD3+WC1+ T cells initially in vaccinated and infected group and then it significantly increased. The WC1+ T cells were more in vaccinated group compared to infected group. In vaccinated and infected groups, both Th1 and Th2 type of immune responses played important role in initiation of immune response, but, in infected group Th2 response dominated. In vaccinated group Th1 response was more as compared to infected group. The production of IFNγ in both the groups suggests the role of cell mediated immunity in the development of immune response. Thus, both humoral and cell mediated immune responses are elicited against live P. multocida intranasal challenge and formalin killed alum adjuvant vaccine. The live bacteria induce more robust local secretory IgA antibody response compared to killed bacteria in vaccine which lead to clearing of bacteria in infected group