CELLULAR, IMMUNOLOGICAL AND MOLECULAR RESPONSES AGAINST PASTEURELLA MULTOCIDA IN CALVES
Loading...
Date
2016-01-23
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
CSKHPKV, Palampur
Abstract
ABSTRACT
The present study determined the cellular, molecular and immunological
responses following P. multocida infection and vaccination in calves. Four months old
nine clinically healthy male cross bred calves were divided into three groups. Calves in
vaccinated group were immunized with 2 ml commercial formalin killed alum adjuvant
HS vaccine s/c, infected group challenged i/n with 5ml of the inoculum containing 3×109
cfu/ml/nostril of P. multocida B:2 and control group was given 5 ml PBS i/n/nostril. The
clinical observations were monitored and serum, nasal secretions and whole blood
samples were collected. The mean rectal temperature and mean total clinical score of the
infected group was significantly higher than other groups from day 3 to 8 and day 2 to 20,
respectively. The serum IgG and IgM response and IgA response in nasal secretions in
the infected group was significantly higher than vaccinated group. The CD3+, CD4+,
CD8+, CD3+CD4+ and CD3+CD8+ T cells increased in both vaccinated and infected
group, however the increase was significant only in infected group. There was decrease
in WC1+ and CD3+WC1+ T cells initially in vaccinated and infected group and then it
significantly increased. The WC1+ T cells were more in vaccinated group compared to
infected group. In vaccinated and infected groups, both Th1 and Th2 type of immune
responses played important role in initiation of immune response, but, in infected group
Th2 response dominated. In vaccinated group Th1 response was more as compared to
infected group. The production of IFNγ in both the groups suggests the role of cell
mediated immunity in the development of immune response. Thus, both humoral and cell
mediated immune responses are elicited against live P. multocida intranasal challenge
and formalin killed alum adjuvant vaccine. The live bacteria induce more robust local
secretory IgA antibody response compared to killed bacteria in vaccine which lead to
clearing of bacteria in infected group
Description
Keywords
null