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  • ThesisItemOpen Access
    Anatomical studies on the intestings of gaddi sheep
    (CSKHPKV, Palampur, 2006) Rajput, Rajesh; Bhardwaj, R.L.
  • ThesisItemOpen Access
    PRENATAL STUDY ON THE DEVELOPMENT OF LIVER OF GADDI SHEEP
    (CSKHPKV, Palampur, 2015-10-15) Razvi, Raheeqa; Rajput, Rajesh
    ABSTRACT Gross morphological, histological and histochemical studies were done on prenatal liver of Gaddi sheep. Total sixty samples were used for gross morphological study and from this twenty four samples were used for histological and histochemical studies. The prenatal liver samples were divided into four groups viz. group-I (1-60 days), group-II (61-90 days) and group-III (91- 120 days) and group-IV (121- full term) having 15 embryos/foeti in each group for gross morphological study and 6 samples in each group for histological and histochemical study. The prenatal liver consisted of two surfaces (visceral and parietal surface), two extremities (cranial and caudal) and two borders (lateral and medial border). Till full term, the liver was not completely oriented towards the right side of abdominal cavity. The mean body weight of sheep foetii increased significantly from group-I to group-IV. Liver accounted 9.07%, 8.45%, 6.15% and 5.49% of the total body weight in group-I, II, III and IV respectively. All biometrical parameters showed an increasing trend with the advancement of age except relative weight of liver and shape index. The right lobe of liver was largest followed by left and caudate lobe. First appearance of portal triad was observed at 76 days of gestation. Right lobe had maximum capsular thickness. Varying concentration of sudanophillic lipids and mucopolysaccharides were observed in different components of liver. In haemopoietic cells and hepatocytes the neutral mucopolysacchariders were present. The presence of bile pigments and glycogen was visible from 57 days of gestation. Iron particles were visisble from 97 days of gestation and bile canaliculi at 53 days of gestation. The concentration (ppm) of Mn, Co, iron, Se and Mo increased with the progression of gestation age. The Zn concentration was maximum between 61-90 days of gestation.While, the concentration of Cu and Ca was maximum between 1-60 days of gestation. Electron microcopy revealed that chromatin material became concentrated and there was decrease in number of mitochondria in post natal hepatocytes than the prenatal ones. The hepatocytes were clearly binucleated. Gall bladder appeared at 46 days of gestation. At 60 days of gestation the tunica mucosa, tunica submucosa, tunica muscularis and tunica serosa layers were clearly distinguishable. The thickness of tunica muscularis, tunica serosa and luminal diameter of gall bladder increased significantly with the advancement of gestational age. While, the thickness of tunica mucosa and submucosa was maximum in group-III. The presence of bile in gall bladder was observed from 53 days of gestation. Chenodeoxycholic acid, cholic acid and deoxycholic acid were three main bile acids identified in bile.
  • ThesisItemOpen Access
    PRENATAL STUDY ON THE DEVELOPMENT OF TESTIS AND EPIDIDYMIS OF GADDI SHEEP
    (CSKHPKV, Palampur, 2015-10-19) Shukla, Parul; Rajput, Rajesh
    Abstract The present study was conducted on the testis and epididymis of 68 Gaddi sheep foetii to study gross anatomy, histogenesis, organogenesis and histochemistry in different stages of gestation. The biochemical analysis was done in the foetal fluid. The samples were divided into 4 stages: stage I (31-60 days), stage II (61-90 days), stage III (91-120 days) and stage IV (121 till term) based on their age in days obtained by the formula, Y = 2.74 x X + 30.15, where X is CRL in cm and Y is age in days. The testes were located under the mesonephric kidneys which started descending into the inguinal canal at around 83rd day of gestation and started entering into the scrotum at around 95th day of gestation and finally settled into the scrotum at around 140th day of gestation. All the gross biometrical parameters increased with the increase in age of the fetus. The differentiation of cortex and medulla in the indifferent gonad was observed on 37th day of gestation. The differentiation of tunica albuginea and seminiferous tubules containing large, small and sertoli cells was observed on 46th day of gestation. The differentiation of tunica albuginea into tunica fibrosa and tunica vasculosa was evident on 50th day of gestation. The connective tissue trabeculae divided the testicular parenchyma into lobules which contained developing sex cords on 60th day of gestation. The testicular capsule and connective tissue trabeculae contained abundant reticular and collagen fibers in stage IV of gestation. The diameter of seminiferous tubules, large, small, sertoli and leydig cells increased with the progression of gestation. The epididymal duct appeared grossly as a straight elongated tubular structure adjacent to the testis on 45th day of embryonic life. The epididymal duct was distinguishable into caput, corpus and cauda regions on 70th day of gestation. The gubernaculums appeared as a cylindrical gelatinous mass and was visible in the scrotum on 86th day of gestation and appeared as a regressed knob like structure on 99th day of gestation. The gross parameters increased with increase in age of Gaddi sheep foetii. The formation of the tunica albuginea in the epididymis was observed on 65th day of gestation and its differentiation into tunica fibrosa and tunica vasculosa was evident on 70th day of gestation. The connective tissue septae originated from the tunica albuginea and divided the epididymis into lobules at 115th day of gestation. Abundant reticular and collagen fibers were seen in the capsule on 137th day of gestation. The tubular and luminal diameter increased significantly from stage II to III and thereafter decreased at stage IV of gestation. The lining epithelium of epididymal tubules was tall cuboidal type. Histochemical reactions showed presence of glycogen, sulphated mucosubstances, lipids and proteins mainly in the capsule, seminiferous tubules and interstitial tissue of the testis and in the capsule, peritubular connective tissue layer, basement membrane, lining epithelium and supra nuclear zone of the epididymis in these areas. Normal values of various macro and micro minerals, biochemicals and activity of various enzymes was analysed in foetal fluid of Gaddi sheep.
  • ThesisItemOpen Access
    PRENATAL STUDY ON THE DEVELOPMENT OF TESTIS AND EPIDIDYMIS OF GADDI SHEEP
    (DEPARTMENT OF VETERINARY ANATOMY, 2015) SHUKLA, PARUL; Rajput, Rajesh
    The present study was conducted on the testis and epididymis of 68 Gaddi sheep foetii to study gross anatomy, histogenesis, organogenesis and histochemistry in different stages of gestation. The biochemical analysis was done in the foetal fluid. The samples were divided into 4 stages: stage I (31-60 days), stage II (61-90 days), stage III (91-120 days) and stage IV (121 till term) based on their age in days obtained by the formula, Y = 2.74 x X + 30.15, where X is CRL in cm and Y is age in days. The testes were located under the mesonephric kidneys which started descending into the inguinal canal at around 83rd day of gestation and started entering into the scrotum at around 95th day of gestation and finally settled into the scrotum at around 140th day of gestation. All the gross biometrical parameters increased with the increase in age of the fetus. The differentiation of cortex and medulla in the indifferent gonad was observed on 37th day of gestation. The differentiation of tunica albuginea and seminiferous tubules containing large, small and sertoli cells was observed on 46th day of gestation. The differentiation of tunica albuginea into tunica fibrosa and tunica vasculosa was evident on 50th day of gestation. The connective tissue trabeculae divided the testicular parenchyma into lobules which contained developing sex cords on 60th day of gestation. The testicular capsule and connective tissue trabeculae contained abundant reticular and collagen fibers in stage IV of gestation. The diameter of seminiferous tubules, large, small, sertoli and leydig cells increased with the progression of gestation. The epididymal duct appeared grossly as a straight elongated tubular structure adjacent to the testis on 45th day of embryonic life. The epididymal duct was distinguishable into caput, corpus and cauda regions on 70th day of gestation. The gubernaculums appeared as a cylindrical gelatinous mass and was visible in the scrotum on 86th day of gestation and appeared as a regressed knob like structure on 99th day of gestation. The gross parameters increased with increase in age of Gaddi sheep foetii. The formation of the tunica albuginea in the epididymis was observed on 65th day of gestation and its differentiation into tunica fibrosa and tunica vasculosa was evident on 70th day of gestation. The connective tissue septae originated from the tunica albuginea and divided the epididymis into lobules at 115th day of gestation. Abundant reticular and collagen fibers were seen in the capsule on 137th day of gestation. The tubular and luminal diameter increased significantly from stage II to III and thereafter decreased at stage IV of gestation. The lining epithelium of epididymal tubules was tall cuboidal type. Histochemical reactions showed presence of glycogen, sulphated mucosubstances, lipids and proteins mainly in the capsule, seminiferous tubules and interstitial tissue of the testis and in the capsule, peritubular connective tissue layer, basement membrane, lining epithelium and supra nuclear zone of the epididymis in these areas. Normal values of various macro and micro minerals, biochemicals and activity of various enzymes was analysed in foetal fluid of Gaddi sheep.