PRENATAL STUDY ON THE DEVELOPMENT OF LIVER OF GADDI SHEEP
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Date
2015-10-15
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CSKHPKV, Palampur
Abstract
ABSTRACT
Gross morphological, histological and histochemical studies were done on prenatal liver of Gaddi
sheep. Total sixty samples were used for gross morphological study and from this twenty four samples
were used for histological and histochemical studies. The prenatal liver samples were divided into four
groups viz. group-I (1-60 days), group-II (61-90 days) and group-III (91- 120 days) and group-IV (121- full
term) having 15 embryos/foeti in each group for gross morphological study and 6 samples in each group for
histological and histochemical study. The prenatal liver consisted of two surfaces (visceral and parietal
surface), two extremities (cranial and caudal) and two borders (lateral and medial border). Till full term, the
liver was not completely oriented towards the right side of abdominal cavity. The mean body weight of
sheep foetii increased significantly from group-I to group-IV. Liver accounted 9.07%, 8.45%, 6.15% and
5.49% of the total body weight in group-I, II, III and IV respectively. All biometrical parameters showed an
increasing trend with the advancement of age except relative weight of liver and shape index. The right
lobe of liver was largest followed by left and caudate lobe. First appearance of portal triad was observed at
76 days of gestation. Right lobe had maximum capsular thickness. Varying concentration of sudanophillic
lipids and mucopolysaccharides were observed in different components of liver. In haemopoietic cells and
hepatocytes the neutral mucopolysacchariders were present. The presence of bile pigments and glycogen
was visible from 57 days of gestation. Iron particles were visisble from 97 days of gestation and bile
canaliculi at 53 days of gestation. The concentration (ppm) of Mn, Co, iron, Se and Mo increased with the
progression of gestation age. The Zn concentration was maximum between 61-90 days of gestation.While,
the concentration of Cu and Ca was maximum between 1-60 days of gestation. Electron microcopy
revealed that chromatin material became concentrated and there was decrease in number of mitochondria in
post natal hepatocytes than the prenatal ones. The hepatocytes were clearly binucleated. Gall bladder
appeared at 46 days of gestation. At 60 days of gestation the tunica mucosa, tunica submucosa, tunica
muscularis and tunica serosa layers were clearly distinguishable. The thickness of tunica muscularis, tunica
serosa and luminal diameter of gall bladder increased significantly with the advancement of gestational age.
While, the thickness of tunica mucosa and submucosa was maximum in group-III. The presence of bile in
gall bladder was observed from 53 days of gestation. Chenodeoxycholic acid, cholic acid and deoxycholic
acid were three main bile acids identified in bile.
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