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20192
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Subject: Agricultural Biotechnology × Author: Eshwar, Jadhav × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Elucidating the role of MYC2 transcription factor in Cucumber mosaic virus infection
    (palampur, 2019-07-30) Eshwar, Jadhav; Hallan, Vipin
    Cucumber mosaic virus (CMV) has the broadest host range among known plant viruses, infecting more than 1,200 species of plants from monocotyledons to dicotyledons. As the effects of CMV is very diverse in nature causing epidemics in diverse crops, it is important to understand the host- pathogen interactions in order to develop plants which can be resistant to plant virus infections. The expression of stress-responsive genes is intimately dependant on its transcriptional control which directly leads to plant response to various stresses. The MYC2 class of transcription factors has recently emerged as a master regulator of the signalling pathways of jasmonic acid (JA) and those of other phytohormones such as abscisic acid (ABA), gibberellins (GAs), and auxin (IAA). Jasmonic acid is the plant hormone that regulates the plant growth, development and defense responses. The MYC2-3 mutant line of Arabidopsis thaliana was used for the analysis of the role MYC2 transcription factor in CMV virus infection. Characterization of MYC2-3 mutant line through jasmonic acid and abscisic acid treatments revealed that the wild type showed reduced root length as compare to MYC2-3 mutant. It was also revealed that the MYC2-3 mutant line has resistance to CMV virus as compared to the wild type plant. Also, SDS PAGE and MALDI-TOF analysis showed that the expression of enzymes and proteins involved in the photosynthetic pathway to be directly affected in the MYC2-3 mutant line on CMV infection. This study could be directly used for development of CMV resistant plant varieties through genome editing in future
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    ThesisItemOpen Access
    ELUCIDATING THE ROLE OF MYC2 TRANSCRIPTION FACTOR IN Cucumber mosaic virus INFECTION.
    (CSKHPKV, Palampur, 2019-07) Eshwar, Jadhav; Hallan, Vipin
    Cucumber mosaic virus (CMV) has the broadest host range among known plant viruses, infecting more than 1,200 species of plants from monocotyledons to dicotyledons. As the effects of CMV is very diverse in nature causing epidemics in diverse crops, it is important to understand the host- pathogen interactions in order to develop plants which can be resistant to plant virus infections. The expression of stress-responsive genes is intimately dependant on its transcriptional control which directly leads to plant response to various stresses. The MYC2 class of transcription factors has recently emerged as a master regulator of the signalling pathways of jasmonic acid (JA) and those of other phytohormones such as abscisic acid (ABA), gibberellins (GAs), and auxin (IAA). Jasmonic acid is the plant hormone that regulates the plant growth, development and defense responses. The MYC2-3 mutant line of Arabidopsis thaliana was used for the analysis of the role MYC2 transcription factor in CMV virus infection. Characterization of MYC2-3 mutant line through jasmonic acid and abscisic acid treatments revealed that the wild type showed reduced root length as compare to MYC2-3 mutant. It was also revealed that the MYC2-3 mutant line has resistance to CMV virus as compared to the wild type plant. Also, SDS PAGE and MALDI-TOF analysis showed that the expression of enzymes and proteins involved in the photosynthetic pathway to be directly affected in the MYC2-3 mutant line on CMV infection. This study could be directly used for development of CMV resistant plant varieties through genome editing in future.