Elucidating the role of MYC2 transcription factor in Cucumber mosaic virus infection
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Date
2019-07-30
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palampur
Abstract
Cucumber mosaic virus (CMV) has the broadest host range among known plant
viruses, infecting more than 1,200 species of plants from monocotyledons to
dicotyledons. As the effects of CMV is very diverse in nature causing epidemics in
diverse crops, it is important to understand the host- pathogen interactions in order to
develop plants which can be resistant to plant virus infections. The expression of
stress-responsive genes is intimately dependant on its transcriptional control which
directly leads to plant response to various stresses. The MYC2 class of transcription
factors has recently emerged as a master regulator of the signalling pathways of
jasmonic acid (JA) and those of other phytohormones such as abscisic acid (ABA),
gibberellins (GAs), and auxin (IAA). Jasmonic acid is the plant hormone that
regulates the plant growth, development and defense responses. The MYC2-3 mutant
line of Arabidopsis thaliana was used for the analysis of the role MYC2 transcription
factor in CMV virus infection. Characterization of MYC2-3 mutant line through
jasmonic acid and abscisic acid treatments revealed that the wild type showed reduced
root length as compare to MYC2-3 mutant. It was also revealed that the MYC2-3
mutant line has resistance to CMV virus as compared to the wild type plant. Also,
SDS PAGE and MALDI-TOF analysis showed that the expression of enzymes and
proteins involved in the photosynthetic pathway to be directly affected in the MYC2-3
mutant line on CMV infection. This study could be directly used for development of
CMV resistant plant varieties through genome editing in future