Thumbnail Image

Theses

Browse

2020 - 20212
Reset filters
Subject: Agricultural Biochemistry × End date: 2023 × Start date: 2020 × Thesis Type: M.Sc ×
Reset filters

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    ThesisItemOpen Access
    SSR markers based genetic diversity studies in Valeriana jatamansi Jones.
    (Palampur, 2021-10-28) Kumari, Nirupma; Sharma, Ram Kumar
    Valeriana jatamansi Jones. is a perennial, tetraploid (2n = 4x = 32), gynodioecious herb from family Valerianaceae. It is native to the Himalayan region, distributed from an altitude range of 1,000 to 3,000 m asl (above sea level) and in India, it is mainly distributed in the northern and north-eastern states. Due to great economic importance for its unique medicinal, flavour and aromatic properties the species have been subjected to great habitat degradation and fragmentation by the illegal trade. Additionally, plant also lacks any molecular genetics efforts mainly because of the non-availability of genome-wide molecular marker resources. In this study, a total of 9,988 SSR containing sequences were derived with maximum abundance of direpeats (~48%) followed by tri-repeats (~32%). Furthermore, synthesis and validation of 103 functionally SSR markers identified 51 polymorphic markers. Extrapolation of highly informative 10 SSR markers for genotyping 100 individuals representing ten diverse geographical populations suggests wide utilization of these markers in large scale fingerprinting studies of V. jatamansi germplasm. Neighbor- joining based hierarchical clustering and Bayesian structure clustering analysis corresponded each other and grouped 100 genotypes into two major clusters suggesting that there are two major genetic pools operating in Himachal Pradesh. Further, AMOVA analysis suggests maximum diversity exists within population (75%). Moderate genetic differentiation (Fst = 0.14) with significant gene flow (Nm = 1.79) may be attributed to natural bottlenecks and various anthropogenic activities in the region. Microsatellite markers identified in this study can be categorized as “Informative Markers” which can be further used for genotyping, genome mapping, evolutionary studies and implementation conservation plans for sustainable utilization of V. jatamansi.
  • Thumbnail Image
    ThesisItemOpen Access
    BIOCHEMICAL AND RESIDUE STUDIES OF TEMBOTRIONE IN MAIZE
    (CSKHPKV. Palampur, 2020-01-31) SAPEHIA, SHAILA; Sharma, Neelam
    A field experiment was laid out in randomized block design (RBD) consisting of five treatments viz. tembotrione 60g/ha, tembotrione 120g/ha, tembotrione 240g/ha, atrazine 1.5kg/ha and control to study the “Biochemical and residue studies of tembotrione in maize” in the Department of Agronomy, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur during kharif season, 2019. Maize plant samples were collected at monthly intervals (i.e. zero (2 hrs), 30, 60 days after the herbicide application) and at harvest for biochemical analysis and grain samples at maturity of crop for quality indices and for residue studies. Soil samples were collected at zero (2 hrs), 1, 3, 5, 7, 10, 15, 30, 45, 60 days and at harvest for the residue studies. Protein content in maize plant was not influenced very distinctly with applied treatments, except at 30 days. However, a numerical increase in total protein content was noticed in tembotrione treatments over control. Total chlorophyll, total carotenoids and total carbohydrates content in maize leaves increased significantly with applied tembotrione treatments and maximum increase was noticed in treatment tembotrione 120g/ha. A significant increase in total carbohydrates content in maize grain over control was observed in all the herbicide applied treatments. The residue data of tembotrione generated in present investigation indicated that tembotrione 60g/ha, tembotrione 120g/ha and tembotrione 240g/ha persisted in soil up to 30, 45 and 45 days, respectively. Approximately 75 per cent of applied herbicide in soil dissipated within 15 days after herbicide application. The dissipation of tembotrione in the soil at three rates of application i.e tembotrione 60g/ha, tembotrione 120g/ha and tembotrione 240g/ha followed first order kinetics and the half –lives ranged from 9 to 14 days. Residues of tembotrione were below detectable levels i.e. ≤0.025µg/g in maize grain and maize stover