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Author: .GOPAKUMAR, CP × End date: 1999 × Start date: 1992 ×
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    ThesisItemOpen Access
    CHROMOSOME PROFILE OF CROSS-BRED BULLS IN KERALA
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCE,MANNUTHY, 1994) .GOPAKUMAR, CP; Raghunandanan, KV
    Cytogenetic study v;as conducted on 53 young cross-bred (Bos taurus x B. indicus) bulls stationed at the farm at Dhoni, belonging to Kerala Livestock Development Board. Young bulls included those selected for superior semen quality and others just started producing semen. The bulls were classified into Jersey cross, Holstein-Friesian cross and Brown-Swiss cross based on the paternal line. The semen quality and related attributes of the bulls were recorded, and the association between these traits and the karyological parameters were determined. Comparative chromosome study v/ere performed in the three genetic groups. Metaphase spreads for staining and G-banding were obtained by peripheral leucocyte culture technique. The basal medium used for culturing was RPMI 1640 and mitosis was initiated in lymphocytes by a combination of phytohemagglutinin and pokeweed mitogen. The G-banding was done by incubating the chromosome spreads in 2 x SSC containing trypsin solution for 45 minutes at 66°C. Karyological parameters such as chromosome number, morphology, relative length, arm ratio and centromericindex were studied. The nature, number and position of G bands 11 were also examined. The reproductive attributes recorded included age at first semen collection age at freezable semen production, volume of semen, sperm concentration, total sperm output in first ten collections, number of ejaculates accepted for freezing and total freezable sperm output in first ten collections, and the morphological abnormalities of sperms.