CHROMOSOME PROFILE OF CROSS-BRED BULLS IN KERALA
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Date
1994
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COLLEGE OF VETERINARY AND ANIMAL SCIENCE,MANNUTHY
Abstract
Cytogenetic study v;as conducted on 53 young cross-bred
(Bos taurus x B. indicus) bulls stationed at the farm at
Dhoni, belonging to Kerala Livestock Development Board.
Young bulls included those selected for superior semen
quality and others just started producing semen. The bulls
were classified into Jersey cross, Holstein-Friesian cross
and Brown-Swiss cross based on the paternal line.
The semen quality and related attributes of the bulls
were recorded, and the association between these traits and
the karyological parameters were determined. Comparative
chromosome study v/ere performed in the three genetic groups.
Metaphase spreads for staining and G-banding were
obtained by peripheral leucocyte culture technique. The
basal medium used for culturing was RPMI 1640 and mitosis was
initiated in lymphocytes by a combination of
phytohemagglutinin and pokeweed mitogen. The G-banding was
done by incubating the chromosome spreads in 2 x SSC
containing trypsin solution for 45 minutes at 66°C.
Karyological parameters such as chromosome number,
morphology, relative length, arm ratio and centromericindex
were studied. The nature, number and position of G bands
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were also examined.
The reproductive attributes recorded included age at
first semen collection age at freezable semen production,
volume of semen, sperm concentration, total sperm output in
first ten collections, number of ejaculates accepted for
freezing and total freezable sperm output in first ten
collections, and the morphological abnormalities of sperms.
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