Thumbnail Image

Thesis

Browse

20141
Reset filters
Subject: Veterinary Pharmacology and Toxicology × Author: DARSANA U. × End date: 2019 ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    ACARICIDAL ACTIVITY OF EXTRACTS AND THE FRACTIONS OF ARTEMISIA NILAGIRICA (CLARKE) PAMP. AND CLERODENDRUM PHILIPPINUM SCHAUER. AGAINST RHIPICEPHALUS (BOOPHILUS) ANNULATUS
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD, 2014) DARSANA U.; Suresh N. Nair
    The present study is envisaged to find out the acaricidal potential of the extract / fractions / subfractions of the plants Artemisia nilagirica (Clarke) Pamp and C. philippinum Schauer. Safety of the extract were assessed by oral (rats) and dermal (rabbits) toxicity studies using relevant OECD guidelines. The efficacy of crude ethanolic extract of A. nilagirica and C. philippinum against R. (B.) annulatus females were assessed by estimating the per cent larval mortality by larval packet test and per cent adult mortality, inhibition of fecundity and larval hatching rate by adult immersion test. The ethanolic extract of A. nilagirica was positive for flavonoids, terpenoids, steroids, glycosides, phenolic compounds, tannins, saponins, fixed oils and fats. The ethanolic extracts of A. nilagirica produced concentration dependent larvicidal activity, adult tick mortality, inhibition of fecundity and highly significant inhibition of hatching. The hexane fraction of this extract retained the acaricidal properties of the extract. The subfraction FA1c was acaricidal among the eight subfractions separated from hexane fraction. Crude extract of C. philippinum demonstrated concentration dependent adult tick mortality and also a significant inhibition of hatching of eggs. The n-butanol fraction of this extract retained the eclosion blocking properties. Qualitative phytochemical analysis of the ethanolic extract of C. philippinum revealed the presence of flavonoids, steroids, glycosides, phenolic compound, tannins, alkaloids, saponins, fixed oils and fats. The subfraction FC3a obtained from the n-butanol fraction of C. philippinum showed significant acaricidal activity. Acute oral, acute and sub-acute dermal toxicity studies did not reveal any toxicity of these two plants. The present work imply that FA1c of hexane fraction of A. nilagirica and FC3a of n-butanol fraction of C. philippinum possessed significant acaricidal activity. The subfraction could be new drug leads for development of promising acaricides.