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  • ThesisItemOpen Access
    Isolation of male sterility and its maintenance in onion (Alliun cepa var. cepa L.)
    (CCSHAU, 2017) Sharma, Pravin Kumar; Avtar Singh
    A field experiment entitled “Isolation of male sterility and its maintenance in onion (Alliun cepa var. cepa L.)” was conducted at Research Farm of Department of Vegetable Science, CCS Haryana Agricultural University, Hisar during rabi season of 2013-14 and 2014-15. Identify and maintain the male sterile line from Onion Hisar 2 variety. Eight cytoplasmic male sterile line (MS 20, MS 21, MS 22, MS 23, MS 34, MS 35, MS 37and MS 40) and two maintainer line (Pollinator 5 and Pollinator 11) was identify with the help of morphologically screening and test cross with fertile pollen donor plant. Identified male sterile line crossed with three tester and developed hybrid for superior general combiner and specific hybrid combination. The analysis of variance exhibited significant genotypic difference, showing considerable amount of genetic variability among the genotypes. The mean squares due to genotypes were found highly significant for all the characters during investigation and suggesting wide difference in the genotypes in the study. PCV were observed to be higher than GCV for all the characters studied, High estimates of PCV and GCV was observed for number of ‘D’ grade bulb followed by number of ‘C’ grade bulbs and number of ‘A’ grade bulbs. Among all eight lines, the line MS 20 was found good general combiner for more number of traits studied. Line MS 34 was found good general combiner for number of leaves per plant, average weight of bulb, total bulb yield, marketable yield, moisture content of bulb (%) and dry matter content of bulb (%). Among the tester Pusa Red showed good general combiner for plant height, diameter of bulb (polar & equatorial), average weight of bulb, total bulb yield, marketable yield, moisture content of bulb (%), dry matter content of bulb (%) and total soluble solids of bulb (%). Tester Pusa Red was observed good combiner for plant height, number of ‘B’ grade bulb and polar diameter of bulb. The positive and desirable heterosis over best parent was exhibited in cross MS 37 x Pusa Red followed by MS 20 x Pusa Red, MS 37 x Hisar 3, MS 22 x Agrifound Dark Red and MS 34 x Pusa Red, respectively for plant height. Higher number of leaves. Positive significant heterosis was observed in the cross MS 21 x Agrifound Dark Red over mid parent and better parent for average bulb weight. positively significant better parent heterosis was observed in the cross MS 20 x Pusa Red followed by MS 37 x Pusa Red, MS 34 x Pusa Red and MS 20 x Hisar 3, respectively for total yield. These crosses could be exploited for the development of onion hybrids and also for selecting desirable segrigants to develop pure line varieties. The preponderance of both additive and non-additive gene actions for yield, its components and quality parameters is greatly suggested for both selection and heterosis breeding for the improvement of onion crop.
  • ThesisItemOpen Access
    Effect of nitrogen sources on production and shelf life of potato
    (CCSHAU, 2017) Taha, Mohamed; Malik, T.P.
    The present investigation was carried out at the Department of Vegetable Science. CCS Haryana Agricultural University, Hisar during Rabi seasons of 2015-16 and 2016-17 to assess the effect of nitrogen sources on production and shelf life of potatovar. Kufri Bahar. The experiment was laid out in a randomized block design with three replications with twelve nutrient management practices as follow T1. Control, T2.100% recommended N (RDN) through farmyard manure (FYM) @ 25.0 t ha-1, T3. 100% RDN through vermicompost (VC) @ 9.3 t ha-1, T4. 100% RDN through neem cake (NC) @ 7.4 t ha-1, T5 75% RDN + 25 % RDN through FYM @ 6.2 t ha-1, T6.75% RDN + 25% RDN through VC @ 2.3 t ha-1, T7. 75% RDN + 25 % RDN through NC @ 1.8 t ha-1, T8. 50% RDN + 25% RDN through FYM @ 6.2 t ha-1 + 25% RDN through VC @ 2.3 t ha-1, T9. 50% RDN + 25% RDN through FYM @ 6.2 t ha-1 + 25% RDN through NC @ 1.8 t ha-1, T10. 50% RDN + 25% RDN through VC @ 2.3 t ha-1 + 25% RDN through NC @ 1.8 t ha-1, T11. 25% RDN + 25% RDN through FYM @ 6.2 t ha-1 + 25% RDN through VC @ 2.3 t ha-1 + 25% RDN through NC @ 1.8 t ha-1 and T12. full recommended dose of NPK (150 kg N: 50 kg P2O5: 100 kg K2O ha-1) through inorganic fertilizer. All the treatment combinations were evaluated for plant growth, yield, quality, and storability parameters. Among the treatments, 50% recommended dose of nitrogen integrated with mixture of 50% recommended dose of nitrogen through organic manures (FYM, NC or VC) showed better performance in terms of growth, yield and its components as compared to control. It was found that crop with the application of 50% RDN through inorganic fertilizer + 25% RDN through FYM @ 6.2 t ha-1 + 25% RDN through VC @ 2.3 t ha-1 followed by 50% RDN through inorganic fertilizer + 25% RDN through VC @ 2.3 t ha-1 + 25% RDN through NC @ 1.8 t ha-1 had higher vigour potential in terms of plant emergence (%), number of stems per hill, plant height (cm) at 30,60 and 90 days after planting, number of leaves per hill at 30, 60 and 90 days after planting, fresh weight of stems, leaves and tubers (g hill-1) at harvest stage, dry weight of stems, leaves and tubers (g hill-1) at harvest stage and NPK uptake (kg ha-1) on haulms and tubers at harvest stage as compared to control. The treatment 50% RDN through inorganic fertilizer + 25% RDN through FYM @ 6.2 t ha-1 + 25% RDN through VC @ 2.3 t ha-1 followed by 50% RDN through inorganic fertilizer + 25% RDN through VC @ 2.3 t ha-1 + 25% RDN through NC @ 1.8 t ha-1 showed significantly higher biological yield and total tuber yield (q ha-1) in comparison to other treatment combinations. Higher marketable yield was observed in nutrient combinations of 50% RDN through inorganic fertilizer + 25% RDN through FYM @ 6.2 t ha-1 + 25% RDN through VC @ 2.3 t ha- 1 followed by 75% RDN through inorganic fertilizer + 25% RDN through VC @ 2.3 t ha-1. The maximum weight (kg m-2) and number of grade-A (≥ 75 g) and grade-B (≥ 51-75 g) size tubers were recorded via using 50% RDN through inorganic fertilizer + 25% RDN through FYM @ 6.2 t ha-1 + 25% RDN through VC @ 2.3 t ha-1 followed by 50% RDN through inorganic fertilizer + 25% RDN through VC @ 2.3 t ha-1 + 25% RDN through NC @ 1.8 t ha-1 or 75% RDN through inorganic fertilizer + 25% RDN through VC @ 2.3 t ha-1. However, grade-C (≥ 26-50 g) size tubers by weight and number was noticed maximum with using 100% recommended nitrogen through vermicompost. Application of 50% RDN through inorganic fertilizer + 25% RDN through FYM @ 6.2 t ha-1 + 25% RDN through VC @ 2.3 t ha-1followed by 50% RDN through inorganic fertilizer + 25% RDN through VC @ 2.3 t ha-1 + 25% RDN through NC @ 1.8 t ha-1showed superior performance over other treatments, recording significantly higher values for harvest index (%), specific gravity and nitrogen use efficiency, whereas significantly higher value of dry matter (%) were recorded in 100% recommended nitrogen dose through vermicompost. The minimum values for physiological loss in weight, sprouting, decay and total loss of potato tubers (%) at 15, 30, 45, 60 and 75 days after harvest was recorded with control and 100% RDN through VC @ 9.3 t ha-1. Net return invested per RS. showed higher performance with using 50% RDN through inorganic fertilizer + 25% RDN through FYM @ 6.2 t ha-1 + 25% RDN through VC @ 2.3 t ha-1 .
  • ThesisItemOpen Access
    Effect of irrigation methods and nitrogen levels and its application on growth and yield of potato
    (CCSHAU, 2017) Maan, Deva Shri; Bhatia, A.K.
    The experiment entitled “Effect of irrigation methods and nitrogen levels and its application on growth and yield of potato” was conducted at Research Farm of the Department of Vegetable Science and in Laboratory of the Department of Soil Science, CCS Haryana Agricultural University, Hisar during Rabi season of 2015-16 and 2016-17. To find out the effect of irrigation methods and nitrogen levels on potato for getting higher yield as well as keeping quality of potato tubers. The potato variety used for the investigation was Kufri Bahar. The treatments comprising of drip and furrow irrigation methods and five recommended dose of nitrogen (60, 80, 100, 120 and 140 % of RDN) were laid out in split plot design with three replications in plot size of 3.60 x 3.60 m2. The ten competitive plants were selected randomly from each treatment to record data on various parameters, which were influenced significantly by different irrigation method and nitrogen levels. The irrigation methods showed significant differences in all the growth and yield parameters expect plant emergence, dry matter in tubers in both the years. Whereas, dose of nitrogen had significant effect on all the parameters expect plant height at 30 days after planting and number of shoots per hill during 2015-16 and 2016-17, respectively. The maximum value for plant emergence (%), plant height at 30, 60 and 90 days after planting (cm), number of shoots per hill, foliage weight (kg/m2), biological yield (q/ha), marketable yield (q/ha), total tuber yield (q/ha), yield of 50 to 75 g and more than 75 g size tubers per square meter (kg/m2), number 50 to 75 g and more than 75 g size tubers per square meter, harvest index (%), nutrient use efficiency (kg/ha/kg), water use efficiency (kg/ha/cm), dry matter content in tubers and foliage (%), nutrient uptake by tubers and foliage (kg/ha) and soil nutrient (kg/ha) were recorded with 100 % of RDN and drip irrigation.The highest benefit: cost was calculated with 100 % of RDN and drip irrigation. Hence, 100 % of RDN with drip irrigation condition have shown the best treatment combination for potato production under semiarid conditions of Hisar, Haryana.
  • ThesisItemOpen Access
    Genetic divergence studies in coriander (Coriandrum sativum L.)
    (CCSHAU, 2017) Shivaprasad M.K.; Tehlan, S.K.
    The present investigation entitled “Genetic divergence studies in coriander (Coriandrum sativum L.)” was conducted at Research Farm of the Department of Vegetable Science, Chaudhary Charan Singh Haryana Agricultural University, Hisar, during winter season of 2014-15 and the laboratory study was carried out in the Department of Molecular Biology, Biotechnology and Bioinformatics during 2015-16. 113 coriander genotypes collected from different regions were sown in field following randomized block design with three replications. To study DUS characters, the genotypes were observed visually and categorized into different classes based on different morphological traits with the help of crop descriptors. It is concluded that the morphological traits like leaf luster of longest basal leaf, leaf margin of longest basal leaf, leaf colour of longest basal leaf, stem pigmentation, corolla colour, nodal pigmentation, growth habit, seed colour, seed shape, seed size and seed ridge proved best for identification purpose, and from this, it is also evident that there was a sufficient diversity for these traits in genotypes under study. Variability studies reveal that there was a wide range of genetic diversity for yield and its attributing traits in 113 coriander genotypes, which were evaluated under field conditions. The genotypic as well as phenotypic correlations showed that the seed yield per plant had significant and positive association with number of seeds per umbel, umbelletes per umbel, umbels per plant and secondary branches. Path analysis revealed that high positive direct effects were exerted by traits like number of seeds per umbel, umbel per plant, length of longest basal leaves and test weight on seed yield per plant, these are importance in crop improvement, as they directly contribute towards seed yield per plant and in divergence study genotypes of cluster III and VI showed highest mean values for seed yield per plant. Hybridization between these clusters and other with highest mean for yield contributing traits can give promising results. However, for more precise and accurate identification, the modern biotechnological tool like molecular markers could be utilized. RAPD and ISSR were employed to assess the genetic diversity in best performing 48 coriander genotypes, which were selected among 113 genotypes evaluated, which gave the clear picture of diversity existence in genotypes at genetic level as DNA markers are not influenced by environmental effects or plant growth stages, and both RAPD and ISSR markers proved best for assessing genetic diversity.
  • ThesisItemOpen Access
    Characterization and seed vigour assessment in fennel (Foeniculum vulgare L.)
    (CCSHAU, 2017) Deswal, Sumit; Malik, T.P.
    A study was conducted on “Characterization and seed vigour assessment” to identify sixty genotypes under study. Plant morphological characters like plant growth habit, seeds per umbels, umbels per umbellate ,arrangement of seeds in main umbel and yield per plant (g) were found to be the most important diagnostic characters for cultivar identification in fennel. Some seed morphological characters like test weight, seed colour, seed shape and seed splitting habbit were also found helpful to differentiate the fennel cultivars. The differential response of fennel genotypes to different chemical tests such as phenol test, modified phenol test-A, modified phenol test-B, and potassium hydroxide test was found to be very effective for genotype identification. The schematic diagram on the basis of some important morphological traits has higher resolution in terms of differentiating among genotypes. The schematic diagrams based on plant growth characters, seed parameters and chemical test are also very useful for identification of genotypes. Field keys were prepared for each and every genotype on the basis of some highly heritable characters with wide variability. This field keys has a pivotal role to identify genotypes in the field without any use of coastly chemicals and are fairly comparable with their results.