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  • ThesisItemOpen Access
    Development of efficient plant regeneration system in Kinnow mandarin(Citrus nobilis Lour × Citrus deliciosa Tenora) for genetic transformation
    (CCSHAU, 2008) Sataveer; Chauhan, V. S.
    The present study was carried out to develop an efficient plant regeneration system in Kinnow mandarin (Citrus nobilis Lour X Citrus deliciosa Tenora) for genetic transformation. Direct regeneration of shoots and roots without intervention of callus from epicotyl segments collected from in vitro raised seedling and shoot tips collected from in vivo raised plants was observed on MS basal medium supplemented with different cytokinins and auxins. The MS+BAP 1.Omg/l was recorded to be the best treatment for shoot proliferation in in vitro epicotyl explants and in vivo shoot tip explants. The MS + BAP 1.0 mg/1 was observed to be the best treatment for shoot proliferation in in vitro epicotyl explants and shoot tip explants in case of parameters like percent regeneration, number of shoots per explant, shoot length followed by MS+Kin l.0 mg/l. The number of shoots per explant ranged, from 1.20 to 4.20 in epicotyl and 1.0 to 4.20 in shoot tips. The maximum length of shoot was recorded 3.20 cm in case of In vitro epicotyl segments and 2.60 cm in shoot tips on the MS+BAP 1mg/l. In case of rooting medium ½ MS+IBA 1.Omg/l was found to be best for different aspects of root differentiation like number of days required for root initiation (13), per cent rooting (94%) number of roots/planted (4.20), root length (4. 1 cm).
  • ThesisItemOpen Access
    Preparation and storage of strawberry (Fragaria × ananassa Duch) products
    (CCSHAU, 2007) Badal, Jayant S.; GOYAL, R.K.
    The investigation on the preparation and storage of Strawberry (Fragaria × ananassa Duch) cv. Oso Grande products were carried out during the year 2002-2003 at Fruit Technology Laboratory, Department of Horticulture, CCS Haryana Agricultural University, Hisar. The present studies were conducted to standardize the method of preservation of strawberry pulp, preparation of RTS and Jam. The prepared products were evaluated for its changes in its physico-chemical and organoleptic properties during three months storage at an interval of 30 days. Total soluble solids, titrable acidity, total sugar and reducing sugar were found to increase with the increase in storage period whereas, ascorbic acid and pectin were found to decrease during both the temperature (Room temperature and Low temperature). The strawberry pulp can best be Experimental Results preserved by the addition of sodium benzoate and potassium metabisulphite @ 0.1% and 0.05% at low temperature and room temperature, respectively. Strawberry RTS beverages was observed that TSS, total sugar and reducing sugar were increased and titrable acidity, ascorbic acid and organoleptic rating were decreased during storage (Room temperature). Among different treatments RTS beverages prepared form pulp (18%), TSS (14%) and acidity (0.30%) was overall best acceptability. Strawberry jam was observed that TSS, total sugar and reducing sugar were increased and decline in titrable acidity, ascorbic acid and pectin was observed. Jam prepared from 100% strawberry pulp was found best in terms of colour, aroma, texture, taste and overall acceptability. Jam with strawberry pulp 1 kg. superior for processing of strawberry products.
  • ThesisItemOpen Access
    Effect of plant extracts and growth regulators on the shelf life of guava (Psidium guajava L.)
    (CCSHAU, 2005) Sharma, Manoj; Ranjit Kumar
    The investigations on “Effect of plant extracts and growth regulators on the shelf life of guava (Psidium guajava L.)” were carried out during 2003-04 at the Post-harvest Labortary, Department of Horticuture, CCS Haryana Agricultural University, Hisar. Various physical and biochemical parameters were studied to know the efficacy of onion extract, methi seeds extract, GA3 and cytokinin at different concentrations to improve the storage life of guava cv. Hisar Safeda. Post-harvest application of onion extract (100%) followed by methi seeds extract (100%) and onion extract (50%) decreased physiological loss in weight (%) and rotting (%) during storage. The quality of fruits was also appreciably IMPROVED by retaining more firmness, TSS, total sugars, reducing sugars and non-reducing sugars. Post-harvest treatment of GA3 (100 ppm), cytokin (20 ppm) and GA3 (50 ppm) also decreased physiological loss in weight (%) and rotting (%). The quality of the fruit was increased. TSS, total sugars, reducing sugars and non-reducing sugars were increased during storage. It can be concluded that the storage life and quality of guava fruits can be improved by onion extract (100%), methi seeds extract (100%), GA3 (100 ppm) and cytokinin (20 ppm).
  • ThesisItemOpen Access
    Studies on the effect of plant extracts on the shelf life of aonla (Emblica officinalis G.)
    (CCSHAU, 2005) Rajiv Kumar; Singhrot, R.S.
    The present investigation was carried out in the post harvest laboratory of the Department of Horticulture at CCS Haryana Agricultural University during the year 2004-2005. The main objective was to know effect of plant extract on shelf life and quality of aonla during storage at room temperature. The different plant extracts used were neem, eucalyptus, marigold and mahendi 2% and 5% in pre harvest and 5% and 10% concentration in post harvest. Neem 2% and 5% in pre harvest and 5% and 10% in post harvest dip treatment were most effective in checking the PLW and decay loss. There were continuous decrease in specific gravity. TSS, non-reducing sugars and total chlorophyll irrespective of the treatments. However, acidity, ascorbic acid, total sugars, reducing sugars and carotenoids showed an increasing trend. Fruits treated with different treatments were mainly infected with Penicillium sp., Fusarium sp. and Aspergillus sp. Slow occurance of pathogenic fungi was recorded in fruits treated with neem followed by mahendi and eucalyptus, fruits treated with neem effectively retained the TSS, acidity, ascorbic acid, sugars and carotenoid content of the fruits. Neem treated fruits showed least PLW and decay loss.
  • ThesisItemOpen Access
    Effect of spacing and nitrogen on growth, flowering and yield of french marigold (Tagetes patula L.)
    (CCSHAU, 2006) Satyawan Singh; Dudi, O.P.
    The present study was carried out during the year 2004-2005 with a view to standardize the effect of spacing and nitrogen in French marigold (Tagetes patula L.) on growth, flowering, flower yield, seed quality and seed yield under agro climatic conditions of Haryana. To achieve these objectives an experiment was conducted with three levels of spacing (30 cm x 30 cm, 30 cm x 45 cm and 30 cm x 60 cm) and four levels of nitrogen (0, 10, 20 and 30 g m-2). The experiment on spacing and nitrogen requirement of French marigold revealed that maximum plant spread, number of branches per plant, days to bud initiation, number of buds per plant, duration of flowering, number of flowers per plant, flower yield per plant and seed yield per plant were found maximum at the spacing level of 30 cm x 60 cm in combination with a nitrogen dose of 20 g m-2 whereas, flower size, flower weight, 1000 seed weight, standard germination, vigour index-I and vigour index-II was found maximum at a spacing of 30 cm x 45 cm in combination with a nitrogen dose of 20 g m-2.
  • ThesisItemOpen Access
    Studies on preservation of mango products
    (CCSHAU, 2006) Saroj; Ashwani Kumar
    The present investigation was carried out at CCS Haryana Agriculural University during the year 2003-04 with the objective to find out best recipe for mango pickle and chutney and to standardized the best method for preservation of mango pickle and chutney. Four recipes were standardize for preparation of mango pickle and four recipes were tried for preservation of mango chutney. The product were assessed for the nutritional and organoleptically properties at monthly intervals for 90 days. Studies on keeping quality of mango pickles and chutney indicated significant decline in pH with corresponding rise in titrable acidity. As the storage period advanced total sugar and reducings sugar increase in both mango pickle and mango chutney. In both the mango products ascorbic acid content showed a decreasing trend throughout the storage period. Upon storage for 90 days mango pickle showed decrease in moisture content whereas mango chutney showed increasing trend in moisture content due to its hygroscopic nature. As the storage period advanced browning also increase in both the products. Mean scores for organoleptic characterstics of sweet mango pickle showed highest score and all pickles showed improvement in organoleptic acceptability with increase in storage period. Among cutneys spicy grated chutney had the highest score than that of others due to its higher acidity, total sugar, reducing sugar and overall acceptability and the mean score for organoleptic acceptability of mango chutney decreased with an increase in storage period. Both the mango products i.e. mango pickle and mango chutney were organolpetically acceptable even after 90 days of storage period and were in the category of “liking above moderate”.
  • ThesisItemOpen Access
    Germination and transplanting studies in aonla (Emblica officinalis gaertn.)
    (CCSHAU, 2006) Rashmi Kumari; Sindhu, S.S.
    The present investigation was carried out at the experimental orchard of Department of Horticulture CCS Haryana Agricultural University, Hisar. In the experiment, the effect of sowing time (3rd week of March and 3rd week of April) and chemicals (GA3 – 250, 500, 750 ppm; Thiourea 0.5, 1.0, 1.5% and KNO3 – 0.5, 1.0, 1.5%) on seed germination in aonla and the transplanting of budded aonla plants with earth ball and without ball during 1st week of July, August, September, January, February and March were studied During the present investigation 500 ppm GA3 was found to be the most effective treatment resulting in significant increase in seed germination (75.50%), survival percentage (83.37%), seedling height (72.94 cm), seedling girth (0.63 cm), number of branches per plant (3.13 cm), percent buddable seedling (80.44%) and early germination (8.0 days) followed by 1.0% thiourea. The seeds soaked in water also gave better performance as compared to untreated seeds. The 3rd week of March was found to be the best time of sowing of seeds as compared to 3rd week of April sowing. The transplanting of budded aonla plants with earth ball was found to be the best treatment as compared to plants without earth ball in all the months. The maximum survival percentage was obtained in plants with earth ball transplanted during 1st week of February (80.00%) followed by 1st week of July (73.30%), whereas, plants without earth ball gave maximum survival percentage when transplanted was done during 1st week of February (73.30%).
  • ThesisItemOpen Access
    Effect of indole butyric acid and callusing period on rooting and growth of kair (Capparis decidua L.) cuttings
    (CCSHAU, 2006) Kamal Kumar; Sharma, Jeet Ram
    The present investigation on “Effect of Indole Butyric Acid and callusing period on rooting and growth of Kair (Capparis decidua L.) cuttings” was carried out during 2005-06, in the nursery of the Centre for Research and Application and Plant Tissue Culture, CCS HAU, Hisar. The mature cuttings were collected during November-December and after giving treatment with different concentrations of IBA (0, 1000, 1500, 2000, 2500, 3000, 3500 and 4000 ppm) for one hour, all the cuttings except 0 day were buried in soil approximately 3 feet deep in optimum moist sand for callus formation. Initiation and duration of sprouting (days) of cuttings were reduced with the application of growth regulator. The cuttings treated with IBA 4000 ppm took minimum number of days followed by IBA 3500 ppm in the initiation of sprouting of cuttings. Percentage of sprouting and survival increased significantly with the IBA treatment over control. Higher number of roots per cutting, average root length, maximum (longest) root length and maximum root number per cutting was recorded with 4000 ppm IBA followed by 3500 ppm IBA treatmen. Height and girth of main shoot were significantly higher in cuttings treated with 4000 ppm followed by 3500 ppm over control treatment. Further callusing treatment has been reported to reduce root and shoot growth as compared to without callusing treatment. The cutting with 60 days callusing period did not sprout because of heavy infestation of fungi, which damaged the callus formation. The cuttings planted with 0 day callusing (without callusing) treatment sprouted much earlier than the cuttings planted 30 and 45 days after callusing. The degree of success in terms of sprouting and survival percentage, number of shoots per cutting, height of main shoot, girth of main shoot, initiation of sprouting of cutting, average root length, maximum (longest) root length, number of roots per cutting, fresh and dry weight of shoot, fresh and dry weight of roots were significantly better in 0 day (without callusing) callusing treatment than 30 and 45 days callusing period.
  • ThesisItemOpen Access
    Assessment of different cultivars of ber (Ziziphus mauritina Lamk.) for their shelf life
    (CCSHAU, 2006) Rakesh Kumar; Sharma, R.K.
    The present investigation “Assessment of different cultivars of their ber (Ziziphus mauritana Lamk.) for their shelf life" was carried out in Post Harvest Technology Laboratory, Department of Horticulture, CCS Haryana Agricultural University, Hisar. Ber fruit of cultivars Sandhura Narnaul, Gola, Narma, Chonchal, Sanaur-5, Kaithli, Banarsi Karaka, Muria mahrana, Umran, ZG-3, Kathaphal, Illaichi were harvest at green mature stage and stored at 250C and 100C. It has been observed that PLW, decay loss and ripening of fruits increased during storage at both the temperature. Among these cultivars stored minimum PLW, decay and ripening was observed in cultivar Umran followed by Sanaur-5, Kaithli and Sandhur Narnaul at 250C. Whereas, 10oC storage was most effective in reducing PLW, decay and ripening as compared to 25 oC. Ripening started on 2nd day at 25 oC and on 4th day at 10 oC. Decay loss was started on 2nd day at 25 oC and on 8th day at 10 oC. Among different cultivars minimum decay loss was recorded in Umran followed by Sanura-5 and Muria Mahrara while it was maximum in Illaichi followed by Chonchal and Narma at 250C. Whereas, minimum decay loss was recorded in Sanaur-5 followed by Urman and ZG-3 and maximum in Illachi followed by Chonchal and Kathaphal on 20th day at 100C. The fruit firmness decreased progressively with increase in storage period at both the temperature. On 8th day storage maximum fruit firmness was observed in Umran followed by Sanaur-5 and Muria Mharara minimum in Illaichi followed by Narma and Chonchal at 250C. Whereas, at 100C maximum was recorded in Umran followed by Sanaur-5 and Gola minimum firmness was in Narma followed by Illaichi and Chonchal on 20th day storage. The continuous decreased in specific gravity was observed at 250C and 10 oC the rate of decreasing was faster and higher at 25 oC and compared to 10 oC. Maximum specific gravity was recorded in Umran followed by Sanaur-5 and Banarsi Karaka and minimum in Illaichi followed by Kathaphal and Chonchal at 250C on 8th day. Whereas, at 100C maximum specific gravity was recorded in Umran followed by Sandhura Narnaul and Kaithli and minimum in Illaichi followed Khathaphal and Chonchal. TSS, total sugar, reducing sugar and non reducing sugar were found maximum Illaichi followed by Chonchal and Kaithli and minimum in Narma, Sandhura Narnaul and ZG-3 on 8th day at 250C. Whereas at 100C maximum in Illaichi followed by Sanaur-5 and Kaithali and minimum in Narma followed by ZG-3 and Sandhura Narnaul on 20th day of storage. Acidity was recorded maximum in Khataphal followed by Muria Mahrara and ZG-3 while minimum in Narma followed by Gola and Kaithli on 8th day of storage. Maximum acidity content was found in Khataphal followed by Sandhura Narnaul and ZG-3 while it was minimum in Umran followed by Banarsi Karaka and Narma on 20th day of storage at 100C. Ascorbic acid were increased initially and then decreased during later storage at 250C and 10 oC respectively. Maximum ascorbic acid was found in Kaithli followed by Illaichi and Sandhura Narnaul and minimum in Chonchal followed by Narma and Gola on 8th day on 250C. However, cultivar Kaithli exhibited maximum ascorbic acid at 100C storage followed by Illaichi and Khathaphal on 20th day of storage. Phenols were founds maximum in Khathaphal followed by Muria Mahrara and Umran and it was minimum in Chonchal followed by Banarsi Kharaka and Narma at both the temperature i.e. 250C and 100C on 8th and 20th day of storage, respectively. Chlorophyll were found maximum in Umran followed by Sanaur-5 and ZG-3 while it was minimum in Chonchal followed by Illaichi and Khathaphal on 8th day of storage at 250C. At 100C maximum chlorophyll content was recorded in ZG-3 followed by Kaithli and Sanaur-5 while it was minimum in Chonchal followed by Illaichi and Muria Mahrara on 20th day of storage. Carotenoids increased with increase in storage period as well as temperature were recorded maximum in Chonchal followed by Muria Mahrara and Umran and minimum in Banarsi Karaka followed by Kaithli and Kathaphal on 8th day of storage at 250C. Whereas, at 100C maximum carotenoids content was observed in Chonchal, Illaichi and Umran and minimum was recorded in Kaithli followed by Banarsi Karaka and Gola on 20th day of storge.