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  • ArticleItemOpen Access
    In vitro Insecticidal Activity of Essential Oil of Eucalyptus globulus against Musca domestica
    (2016-09) Rani, N; Harikrishnan, T.J.; Ponnudurai, G; TANUVAS
    Essential oil was extracted from the fresh leaves of Eucalyptus (Eucalyptus globulus) by hydrodistillation using Clevenger apparatus at 80° C for 3 - 4 hrs. Fifty gram of fresh leaves yielded maximum of 2 ml of oil. In vitro ef cacy of the essential oil against larvae, pupae and adult  ies at different concentrations viz., 50, 100, 150, and 200 μl was evaluated. The mortality of larvae, pupae and adult ies increased with the concentration of the oil increased. The LD50 values of oil against larvae and pupae were 48.2, and 15.69 μl respectively. In the control group, 10.16 ± 0.31 and 10.16 ± 0.28 per cent mortality of larvae and pupae respectively were observed. The fumigant toxicity against adult ies showed that the highest concentration of 200 μl caused 100 per cent mortality in 6.5 ± 0.24 minutes against no mortality of ies up to 2 days in the control group. The dead larvae from treated group appeared shrunken and black in colour, while histopathological study revealed bleb formation and intestinal cell damage.
  • ArticleItemOpen Access
    Ef cacy of Cypermethrin to Control Haemaphysalis Intermedia Ticks
    (Indian Veterinary Association, 2016-09) Rubinibala, B; Ponnudurai, G; Senthilvel, K; Balasubramaniam, A; TANUVAS
    A study was undertaken to determine the ef cacy of cypermethrin in Haemaphysalis intermedia ticks from smallholder ocks in Namakkal district and organised farms of Veterinary College and Research Institute (VC&RI) and Mecheri Sheep Research Station (MSRS). Bioassays viz., Adult Immersion Test (AIT) and Larval Packet Test (LPT) were used in this study. A signicant reduction in egg mass was observed at higher concentration of cypermethrin. AIT showed that the LC50 and LC95 values were 103.39 ppm and 291.11 pm with 95 per cent con dence interval for ticks from smallholder  ock and 106.15 ppm and 310.92 ppm with 95 per cent con dence interval for ticks from VC&RI farm. LPT revealed that LC50 and LC95 values of larvae from smallholder  ock, VC&RI and MSRS farms were 97.76 and 284.20, 97.57 and 280.41, and 98.38 and 274.37 respectively with 95 per cent con dence interval. High mortality with low reproductive index among the survived ticks and low discriminating doses indicated that H. intermedia ticks were susceptible to cypermethrin.