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ThesisItem Open Access APPLICATION AND EVALUATION OF MESENCHYMAL STEM CELLS ON WOUND HEALING IN RAT(West Bengal University of Animal & Fishery Sciences, Kolkata, 2023) MAHESH A, KUMARA DATTHA; Pradhan, S.; Mondal, S.; Das, K.; Bag, Sadhan; Dey, SamirThe primary goal of this study is to assess the wound healing effect of mesenchymal stem cells and condition media in xenogeneic transplantation on rat skin full thickness excisional wounds. MSCs derived from bovine umbilical cord were isolated and cultured using explant and enzymatic methods. The MSC isolation technique was standardised using enzymatic digestion, as was the explant method. Because of their migratory properties, MSCs emerged from explants by day 4 and reached 70-90% confluence by day 10. MSCs were digested with collagenase (1mg/ml) for 4 hours at 370C before being plated and cultured, reaching 90% confluence after 72 hours. MSCs exhibited strong plastic adherence and fibroblast-like morphology. MSCs were cultured in vitro and differentiated into osteocytes, chondrocytes, and adipocytes according to International Society for Cell Therapy and Mesenchymal Stem Cell Committee guidelines. Xenogeneic bovine UC-MSC and bovine UC-MSC derived condition media were transplanted into two treatment groups and compared to positive, negative (vehicle), and wound healing ointment groups. A full thickness (10mm) skin punch biopsy was performed on both sides of the shoulder region, and MSC and CM were injected at the wound's periphery. Wound healing was evaluated using wound contraction (up to day 14), gross morphological evaluation (up to day 7), cytological examination of the wound bed area (up to day 7), and histo-morphological evaluation of skin samples collected on days 7, 14, 21, and 28. Daily photographs were taken from the same height, and wound closure was accelerated in the MSC and CM treated groups. Gross morphological parameters were scored, and it was discovered that all wounds in all experimental groups were grossly healthy until day 7, with enhanced granulation tissue noted in MSC and CM treated groups. Cytological analysis revealed a significant increase in mononuclear infiltration after 72-96 hours in the CM and MSC groups. Overall, histomorphological observations revealed that MSC and CM treated groups had significantly accelerated wound closure, advanced re-epithelisation and neovascularisation, mature extra-cellular matrix deposition with better collagen arrangement, and reduced scarring. Every parameter was scored and statistically evaluated.ThesisItem Open Access ROLE OF CURCUMINOID AND SESAMOID OIL IN COLON CANCER DEVLOPMENT AND PROGRESSION(West Bengal University of Animal & Fishery Sciences, Kolkata, 2023) SENAPATI, SANJIB; Hansda, R.N.; Mukhopadhayay, S.K.; Pradhan, S.; Mondal, Samiran; Dey, SamirThesisItem Open Access EFFECT OF CHRONIC STRESS ON REPRODUCTIVE CYCLE AND EXPRESSION PATTERN OF RFRP-3 GENE IN ADULT FEMALE RATS(West Bengal University of Animal & Fishery Sciences, Kolkata, 2023) TZUDIR, AKUMMENLA; Banerjee, D; Bag, S.; Das, K.; Mukherjee, J.; Mondal, S.; Polley, S.The present study was conducted to analyze the effect of chronic stress on the reproductive cycle and expression pattern of the RFRP-3 gene in adult female rats. The experiments were performed on adult female Wistar rats which were divided into two groups viz. Control and the Stressed groups, and were housed individually in polypropylene cages. The rats of the Stressed group were subjected to 3 hrs. of restraint or immobilization stress daily in a modified rat restrainer for 28 days. The reproductive cycle of the female rats was monitored and the different stages of the oestrous cycle (proestrus, estrus, metestrus and diestrus) were determined through the visual assessment of the vagina of the rats and the vaginal exfoliated cell cytology. All rats from both the Control and the Stressed groups were tested for behavioural traits after the completion of the trial period. Different tests viz. open field locomotion test, Porsolt’s forced swim test, Light/Dark Transition test and the Elevated plus maze test were performed to observe the anxiety/depressive behaviour of the rats.The experimental animals of both groups were sacrificed at the end of the trial period. Plasma was separated from the collected blood samples and was estimated for haematological parameters (Hb, PCV, TEC, TLC and DLC), biochemical parameters (Blood glucose, Total antioxidant, T3, T4 and Cortisol) and reproductive hormones (LH, FSH, Progesterone and Estradiol). The tissue samples collected for histological studies were ovary and for the study of the expression of the RFRP-3 gene were brain (hypothalamus). During the visual assessment of the vagina, the vaginal opening of the rat appeared to be wide and moist and the tissues appeared pink during proestrus. During estrus, the vagina appeared similar to proestrus but was less pink and moist. In metestrus, a pale and dry vaginal opening was seen and during the proestrus, the vaginal opening was very moist, small and closed. The result of the vaginal cell cytology showed that a mixture of epithelial cells was seen in the proestrus. The estrus stage was characterized mainly by the presence of anucleated cornified (keratinized) cells. Metestrus shows the presence of leucocytes and some keratinized cells. Polymorphonuclear leucocytes were seen in diestrus. The behavioural analysis of the rats showed that the rats of the stressed group showed more depressive/anxiety-like behaviour when compared to the control group. The PCV and Hb of the stressed rats were highly significantly(P<0.01)lower than the control rats. The TLC and the TEC between the two groups were found to be not statistically significant (P>0.05). A significant (P<0.05) variation was observed in the blood glucose, T3, T4 and Cortisol between the two groups. The variation in the total antioxidant was found to be non-significant (P>0.05). A significant (P<0.05) variation was observed in the LH of the two groups. The variation in the mean with standard error of FSH, Estradiol (E2) and Progesterone of the two groups viz. the Control and the stressed group was found to be not statistically significant (p>0.05). A significant (P<0.05) increase in the expression of the RFRP-3 gene was seen in the hypothalamus of the stressed rat. The results lead us to conclude that chronic stress causes significant changes in the normal blood physiology of the rat. Chronic stress also alters the concentration of the reproductive hormones which confirms the relationship between stress-induced female infertility and the expression of RFRP-3.ThesisItem Open Access EFFECT OF DIETARY SUPPLEMENTATION OF VITAMIN E AND SELENIUM ON THE GROWTH, HEMATOBIOCHEMICAL, ENDOCRINE AND IMMUNE STATUS OF GROWER GHUNGROO PIGS(West Bengal University of Animal & Fishery Sciences, Kolkata, 2022) Hussain, Rubayat; Mukherjee, J.; Das, P.K.; Banerjee, Dipak; Batabyal, Subhasis; Bag, SadanThe focus of the present investigation was to highlight the findings of effectiveness of dietary supplementation of vitamin E and selenium in grower Ghungroo pigs on growth, hematobiochemical, endocrine, immunity and antioxidant status. Eighteen healthy female grower Ghungroo pigs (Susscroffa domestica) of 2-3 months were selected randomly for the experiment and divided into three groups. The control group (n=6) fed only the basal diet, animals of treatment-1 (T1) group (n=6) were supplied with vitamin E (in form of α tocopherol acetate) @11IU/Kg feed and animals of treatment-2 (T2) group of animals (n=6) were supplemented with selenium (as sodium selenite) @0.15mg/Kg feed daily as per NRC recommendations. The body weight, morphometric parameters of growth and physiological responses were recorded at the interval of 15 days. Blood samples were collected from all the experimental animals on day 15, day 30, day 45 and day 60 of supplementation. Vitamin E and selenium were estimated in the plasma. Hematological parameters were evaluated as per standard procedures. Blood biochemical, endocrine (cortisol, T3 and T4) and total antioxidant status were evaluated by commercially available kits as per manufacturer’s protocol. In vitro phagocytic activity and lymphocyte proliferation response were evaluated by colorimetric NBT and MTT assay respectively. The supplementation of vitamin E and selenium significantly (P<0.01) increased plasma vitamin E and selenium concentration in grower Ghungroo pigs from 15th day of supplementation and persisted till the end of experiment. The supplementation of vitamin E and selenium did not have any significant effect on the growth related morphometric parameters in Ghungroo pigs. The supplementation of vitamin E increased haemoglobin and MCH concentration whereas, selenium supplemented pigs showed higher PCV, MCV, TEC and TLC. The supplementation of vitamin E significantly increased plasma glucose and decreased AST and ALT whereas, selenium supplementation resulted higher total protein in experimental pigs. Vitamin E-treated group had a considerably greater T3 and T4 level and lower cortisol level than the control and selenium-treated groups. Vitamin E supplementation improved the immune status of the growing Ghungroo pigs in terms of in vitro lymphocyte proliferation response, phagocytic activity of neutrophils along with increased total antioxidant status. In conclusions, the present investigation depicted that dietary supplementation of vitamin E had beneficial effect on the immunity and antioxidant status in grower Ghungroo pigs without affecting normal growth and physio-biochemical functions.ThesisItem Open Access Effects of dietary turmeric and lemon essential oil on weaning and post-weaning performances of Ghoongroo piglets(West Bengal University of Animal & Fishery Sciences, Kolkata, 2022) DAS, ANIL KUMAR; Das, P.K.; Sanyal, Sagar; Mukherjee, J.; Batabyal, Subhasis; Patra, A.; Naskar, S.ThesisItem Open Access MULTIPLE DOSE EXPOSURE STUDY OF Psidium guajava Linn. ON EXPERIMENTALLY INDUCED INFLAMMATORY BOWEL DISEASE IN ANIMAL MODEL(West Bengal University of Animal & Fishery Sciences, Kolkata, 2023) MEHRA, SANDEEP; Hansda, R.N.; Kumar, Deepak; Mukhapadhayay, S.K.; Mondal, Samiran; Pradhan, Saktipada; Batabyal, KunalThe current study attempted to screen Psidium guajava leaves for their influence on experimentally produced inflammatory bowel illness (IBD). Inflammatory bowel disease (IBD) is a prevalent chronic inflammatory gastrointestinal condition. Crohn's disease (CD) and ulcerative colitis are the two most common kinds of IBD (UC). The anti inflammatory and antioxidant properties of Psidium guajava leaves were investigated in DSS-induced IBD. The experiment was performed using Dextran sulphate Sodium (DSS) 2 % DSS is added to the drinking water for 7 consecutive days except in negative controle. Aquous extract of P. guajava will be given for 28 days [from day 1 to day 28 with DSS(7days )] to know the antiinflammatory and antioxidant properties the rats will be divided into following namely group I: negative control, Group II: positive control, Group IlI: P. guajava leaf extract 30mg/kg, Group IV: P. guajava leaf extract 60 mg/kg and Group V: P guajava leaf extract 120mg/kg. Haemoglobin,TEC, TLC, DLC, Total Platelet Count, and other common haematological parameters and serum biochemistry such as ALT, AST, Kidney Function Test (crt.and urea) total protein and serum electrolyte sodium were assessed and found maximum of test shows significance difference. In cytology-based analysis, anal cytology for representative group of positive control(dss 2%) were taken every day for 7 consecutive days with using swab method in anal rectal region. In histomorphological of small intestine found that mucosal ulcer ,erosion,necrosis amd infiltration of inflammatory cell.In kidney they have been normal architecture with focal. In liver no histopthological changes except glycogen accumulation in liver. In gross pathology inflammatory nodule is seen on 1st and 2nd week of experiment. Histopathological changes of liver, kidney and intestine were significantly reduced by aqueous extract of Psidium guajava in last week of experiment. Therefore psidium guajava has a good protective potential in inflammatory bowel disease.ThesisItem Open Access STUDIES ON CONSTRUCTION OF VIABLE INVERSE - SKIN TUBE FOR USE AS AN IDEAL OESOPHAGEAL SUBSTITUTE IN CANINES(Kolkata, 2000) SINGH, BHUPINDER; Sen, Dr. T. B.; Bose, Prof. (Dr) P. K.; Samanta, Dr. P. K.; Biswas, Prof. (Dr) B.ThesisItem Open Access STUDIES ON SOME ASPECTS OF ADULT BEHAVIOUR OF FOWL ON DEEP LITTER(kolkata, 2011) MODHUKOILYA, AYUSHMAN; Samanta, Prof. A. K.; Samanla, Prof. R.; Misra, Prof. S. K.ThesisItem Open Access Studies on prevalence, pathology haemato - biochemical changes of canine demodicosis in and arround kolkata(Kolkata, 2002) Das, Liton; Mukhopadhay, Dr. S .K.; Som, Dr. T. L.; Bhowmiki, Prof. M. K.; Dasgupta, Prof.C. K.; Deb, Prof. (Mrs.) P.; Roy, Prof. (Mrs.)
