EFFECT OF DIETARY SUPPLEMENTATION OF VITAMIN E AND SELENIUM ON THE GROWTH, HEMATOBIOCHEMICAL, ENDOCRINE AND IMMUNE STATUS OF GROWER GHUNGROO PIGS
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Date
2022
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West Bengal University of Animal & Fishery Sciences, Kolkata
Abstract
The focus of the present investigation was to highlight the findings of effectiveness of dietary
supplementation of vitamin E and selenium in grower Ghungroo pigs on growth, hematobiochemical,
endocrine, immunity and antioxidant status. Eighteen healthy female grower
Ghungroo pigs (Susscroffa domestica) of 2-3 months were selected randomly for the
experiment and divided into three groups. The control group (n=6) fed only the basal diet,
animals of treatment-1 (T1) group (n=6) were supplied with vitamin E (in form of α tocopherol
acetate) @11IU/Kg feed and animals of treatment-2 (T2) group of animals (n=6) were
supplemented with selenium (as sodium selenite) @0.15mg/Kg feed daily as per NRC
recommendations. The body weight, morphometric parameters of growth and physiological
responses were recorded at the interval of 15 days. Blood samples were collected from all the
experimental animals on day 15, day 30, day 45 and day 60 of supplementation. Vitamin E and
selenium were estimated in the plasma. Hematological parameters were evaluated as per
standard procedures. Blood biochemical, endocrine (cortisol, T3 and T4) and total antioxidant
status were evaluated by commercially available kits as per manufacturer’s protocol. In vitro
phagocytic activity and lymphocyte proliferation response were evaluated by colorimetric NBT
and MTT assay respectively. The supplementation of vitamin E and selenium significantly
(P<0.01) increased plasma vitamin E and selenium concentration in grower Ghungroo pigs
from 15th day of supplementation and persisted till the end of experiment. The supplementation
of vitamin E and selenium did not have any significant effect on the growth related
morphometric parameters in Ghungroo pigs. The supplementation of vitamin E increased
haemoglobin and MCH concentration whereas, selenium supplemented pigs showed higher
PCV, MCV, TEC and TLC. The supplementation of vitamin E significantly increased plasma
glucose and decreased AST and ALT whereas, selenium supplementation resulted higher total
protein in experimental pigs. Vitamin E-treated group had a considerably greater T3 and T4
level and lower cortisol level than the control and selenium-treated groups. Vitamin E
supplementation improved the immune status of the growing Ghungroo pigs in terms of in
vitro lymphocyte proliferation response, phagocytic activity of neutrophils along with
increased total antioxidant status. In conclusions, the present investigation depicted that dietary
supplementation of vitamin E had beneficial effect on the immunity and antioxidant status in
grower Ghungroo pigs without affecting normal growth and physio-biochemical functions.