MULTIPLE DOSE EXPOSURE STUDY OF Psidium guajava Linn. ON EXPERIMENTALLY INDUCED INFLAMMATORY BOWEL DISEASE IN ANIMAL MODEL
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Date
2023
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West Bengal University of Animal & Fishery Sciences, Kolkata
Abstract
The current study attempted to screen Psidium guajava leaves for their influence on
experimentally produced inflammatory bowel illness (IBD). Inflammatory bowel disease
(IBD) is a prevalent chronic inflammatory gastrointestinal condition. Crohn's disease (CD)
and ulcerative colitis are the two most common kinds of IBD (UC). The anti inflammatory
and antioxidant properties of Psidium guajava leaves were investigated in DSS-induced IBD.
The experiment was performed using Dextran sulphate Sodium (DSS) 2 % DSS is added to
the drinking water for 7 consecutive days except in negative controle. Aquous extract of P.
guajava will be given for 28 days [from day 1 to day 28 with DSS(7days )] to know the antiinflammatory
and antioxidant properties the rats will be divided into following namely group
I: negative control, Group II: positive control, Group IlI: P. guajava leaf extract 30mg/kg,
Group IV: P. guajava leaf extract 60 mg/kg and Group V: P guajava leaf extract 120mg/kg.
Haemoglobin,TEC, TLC, DLC, Total Platelet Count, and other common haematological
parameters and serum biochemistry such as ALT, AST, Kidney Function Test (crt.and urea)
total protein and serum electrolyte sodium were assessed and found maximum of test shows
significance difference. In cytology-based analysis, anal cytology for representative group of
positive control(dss 2%) were taken every day for 7 consecutive days with using swab
method in anal rectal region. In histomorphological of small intestine found that mucosal
ulcer ,erosion,necrosis amd infiltration of inflammatory cell.In kidney they have been normal
architecture with focal. In liver no histopthological changes except glycogen accumulation in
liver. In gross pathology inflammatory nodule is seen on 1st and 2nd week of experiment.
Histopathological changes of liver, kidney and intestine were significantly reduced by
aqueous extract of Psidium guajava in last week of experiment. Therefore psidium guajava
has a good protective potential in inflammatory bowel disease.