Thumbnail Image

Theses (Ph.D.)

Browse

2014 - 20152
 Reset filters

Settings

Thesis Type: M.F.Sc ×

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    ThesisItemOpen Access
    Immune Gene Expression in Tiger Shrimp(Penaeus Monodon) to Vibrio Harveyi Infection
    (Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, 2015-01-15) Dechamma, M. M.; Indrani Karunasagar; Otta, S.K.; Venugopal, M.N.; Girish, S.K.; Maragal, M.M.; Krishna Bhatt, C.H.
    Culture of penaeid shrimp is an important economic activity in most of the Asian and Latin American countries. Crustacean aquaculture is showing an increasing trend but disease problems are a major constraint. Vibriosis is the most significant bacterial infectious disease in the aquaculture sector caused by members of Vibrio harveyi clade. Crustaceans resist the pathogen by the innate immune response as they lack the classical adaptive immune system though a primitive form of memory is being unravelled. Thus, in view of developing strategies to disease management, the present study throws light on the identification and expression of immune gene in black tiger shrimp P. monodon to V. harveyi infection to harness their potential. The shrimps were immune stimulated with β-glucan to see if the immune molecules were enhanced. Following immune-stimulation they were infected with V. harveyi. Selected novel immune genes were tested for the tissue distribution and expression profiling using semi- quantitative reverse transcriptase PCR. Akirin and thrombospondin genes were found to be constitutively expressed in all tissue samples such as gill, hepatopancreas, lymphoid organ, hemolymph, eye stalk, stomach, heart, muscle of healthy P. monodon. Akirin, HSP 60, HSP 70, HSP 90, thrombospondin and toll-like receptors were checked for their expression level in gill, hepatopancreas, lymphoid tissue and hemolymph in both post V. harveyi infected and also in immunemodulated cum infected shrimps. The present study gives an idea of the relative expression levels of immune related gene in normal, infected and immune-stimulated animals.
  • Thumbnail Image
    ThesisItemOpen Access
    Development of Recombinant Protein and RNAI to Protect Shrimp from White Spot Syndrome Virus (WSSV) Infection
    (Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, 2014-05-15) Akhila, D.S.; Indrani Karunasagar; Karunasagar, I.; Pradeep, B.; Venu Gopal, M.N.; Girish, S.K.; Krishna Bhatt, C.H.
    White spot syndrome virus (WSSV) is a highly virulent shrimp pathogen, causing huge economic loss to the aquaculture industry. The possibility of protecting Litopenaeus vannamei against white spot syndrome virus (WSSV) infection via vaccination and RNA interference technology by intramuscular and oral administration was explored. Shrimp orally vaccinated with rVP39 and rVP28 showed a cumulative mortality of 50 and 60% respectively following challenge. rVP39 had a better protective effect against WSSV infection compared to rVP28. Vaccination by intramuscular injection with rVP39 and rVP28 resulted in survival rate of 70 and 60% respectively. Various genes of WSSV envelop protein (VP28 and VP39), structural protein (RR1) and the shrimp protein PmRab7 were targeted for the production of dsRNA. The results of both oral and injection mode of delivery of dsRNA showed higher percentage and longer period of survival when using RR1 in comparison with the other targeted genes of dsRNA. The present study demonstrates the protective efficacy of vaccination and RNAi strategy which mainly depends on the role and significance of protein encoded by the target gene in the viral life cycle.