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    ThesisItemOpen Access
    STUDIES ON MICROFILARIOSIS IN DOGS WITH SPECIAL REFERENCE TO SEROLOGICAL AND MOLECULAR DIAGNOSIS
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2019-11-01) MALATESH, D. S.; C. ANSAR KAMRAN
    A study was conducted to determine prevalence of microfilariosis in dogs belonging to Shivamogga a Malnad region and Mangaluru a coastal region of Karnataka for a period of one year from March-2018 to February-2019. In the present study, out of 315 dog blood samples, 3.9 and 44.4 per cent were found positive for microfilariosis from Shivamogga and Mangaluru region respectively. Only one filarial parasite species D. repens was detected in dogs from Shivamogga and Mangaluru region. The highest prevalence of microfilariosis was observed in 3-6 years old male dogs living near farm area and outdoor as well as during North-east monsoon and winter seasons. The breed-wise prevalence of microfilariosis was found highest in Boxer breed dogs from Shivamogga whereas, Labrador, Doberman and Golden Retriever breeds showed highest prevalence from Mangaluru. Quantitative buffy coat analysis found to be most sensitive method in detection of microfilaria in blood. Microfilaria detected from the infected dogs were identified as Dirofilaria repens based on morphological studies using modified Knott’s method and Giemsa’s staining technique. In the present study, variation in the haematological and biochemical parameters of microfilaremic dogs compare to control dogs was not significant. Out of 315 serum samples, seroprevalence of microfilariosis recorded in the present study using microfilarial and adult worm antigen was 69.84 and 58.09 per cent by ELISA respectively. The species of microfilaria and adult worm was identified as D. repens by PCR using both panfilarial primers and D. repens specific primers. Among four drugs used in the present study, selamectin showed good efficacy in treatment of microfilariosis in dogs compare to doxycycline, ivermectin and melarsomine dihydrochloride. Keywords: Microfilariosis, Dog, Shivamogga, Mangaluru, Seroprevalence, PCR, Treatment.
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    ThesisItemOpen Access
    STUDIES ON MICROFILARIOSIS IN DOGS WITH SPECIAL REFERENCE TO SEROLOGICAL AND MOLECULAR DIAGNOSIS
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2019-11) MALATESH, D. S.; C. ANSAR KAMRAN
    A study was conducted to determine prevalence of microfilariosis in dogs belonging to Shivamogga a Malnad region and Mangaluru a coastal region of Karnataka for a period of one year from March-2018 to February-2019. In the present study, out of 315 dog blood samples, 3.9 and 44.4 per cent were found positive for microfilariosis from Shivamogga and Mangaluru region respectively. Only one filarial parasite species D. repens was detected in dogs from Shivamogga and Mangaluru region. The highest prevalence of microfilariosis was observed in 3-6 years old male dogs living near farm area and outdoor as well as during North-east monsoon and winter seasons. The breed-wise prevalence of microfilariosis was found highest in Boxer breed dogs from Shivamogga whereas, Labrador, Doberman and Golden Retriever breeds showed highest prevalence from Mangaluru. Quantitative buffy coat analysis found to be most sensitive method in detection of microfilaria in blood. Microfilaria detected from the infected dogs were identified as Dirofilaria repens based on morphological studies using modified Knott’s method and Giemsa’s staining technique. In the present study, variation in the haematological and biochemical parameters of microfilaremic dogs compare to control dogs was not significant. Out of 315 serum samples, seroprevalence of microfilariosis recorded in the present study using microfilarial and adult worm antigen was 69.84 and 58.09 per cent by ELISA respectively. The species of microfilaria and adult worm was identified as D. repens by PCR using both panfilarial primers and D. repens specific primers. Among four drugs used in the present study, selamectin showed good efficacy in treatment of microfilariosis in dogs compare to doxycycline, ivermectin and melarsomine dihydrochloride.
  • Thumbnail Image
    ThesisItemOpen Access
    STUDIES ON MICROFILARIOSIS IN DOGS WITH SPECIAL REFERENCE TO SEROLOGICAL AND MOLECULAR DIAGNOSIS
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2019-11) MALATESH, D. S.; C. ANSAR KAMRAN
    A study was conducted to determine prevalence of microfilariosis in dogs belonging to Shivamogga a Malnad region and Mangaluru a coastal region of Karnataka for a period of one year from March-2018 to February-2019. In the present study, out of 315 dog blood samples, 3.9 and 44.4 per cent were found positive for microfilariosis from Shivamogga and Mangaluru region respectively. Only one filarial parasite species D. repens was detected in dogs from Shivamogga and Mangaluru region. The highest prevalence of microfilariosis was observed in 3-6 years old male dogs living near farm area and outdoor as well as during North-east monsoon and winter seasons. The breed-wise prevalence of microfilariosis was found highest in Boxer breed dogs from Shivamogga whereas, Labrador, Doberman and Golden Retriever breeds showed highest prevalence from Mangaluru. Quantitative buffy coat analysis found to be most sensitive method in detection of microfilaria in blood. Microfilaria detected from the infected dogs were identified as Dirofilaria repens based on morphological studies using modified Knott’s method and Giemsa’s staining technique. In the present study, variation in the haematological and biochemical parameters of microfilaremic dogs compare to control dogs was not significant. Out of 315 serum samples, seroprevalence of microfilariosis recorded in the present study using microfilarial and adult worm antigen was 69.84 and 58.09 per cent by ELISA respectively. The species of microfilaria and adult worm was identified as D. repens by PCR using both panfilarial primers and D. repens specific primers. Among four drugs used in the present study, selamectin showed good efficacy in treatment of microfilariosis in dogs compare to doxycycline, ivermectin and melarsomine dihydrochloride. Keywords: Microfilariosis, Dog, Shivamogga, Mangaluru, Seroprevalence, PCR, Treatment.