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20193
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Author: AKSHATA S. ANGADI ×

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    ThesisItemOpen Access
    PATHOMORPHOLOGICAL AND MOLECULAR EVALUATION OF METHOTREXATE (MTX) INDUCED TOXICITY AND ITS AMELIORATION BY ZINC OXIDE (ZnO) NANOPARTICLES
    (KARNATAKA VETERINARY, ANIMAL & FISHERIES SCIENCES UNIVERSITY, BIDAR, 2019-07-01) AKSHATA S. ANGADI; SUGUNA RAO
    The present study was pursued to evaluate the pathomorphological, biochemical and molecular changes in methotrexate induced toxicity and its amelioration by zinc oxide nanoparticles treatment in rats for 45 days. The study included normal control (Group I), methotrexate control (Group II), zinc oxide nanoparticle control (Group III), zinc oxide nanoparticles pre-treatment (Group-IV), ZnO NP concurrent treatment (Group-V) and silymarin treatment (Group-VI) groups. Toxicity was induced in rats by administration of methotrexate at the dose rate of 5 mg/kg bw IP for three consecutive days and zinc oxide nanoparticles were used at the dose rate of 50 mg/kg bw PO. The parameters assessed following the administration of methotrexate and zinc oxide nanoparticles in different groups included clinical signs, biochemical parameters, antioxidant enzymes, lipid peroxidation assay and gross and histopathology. Molecular study included immunohistochemical evaluation of p53 expression and relative quantification of p53 expression in liver by real time PCR. Methotrexate induced toxicity was characterized by necrosis and apoptosis. The pre-treatment and concurrent zinc oxide nanoparticles administration partially alleviated the methotrexate induced toxic effects. The pre-treatment of zinc oxide nanoparticles rendered better acquisition of overall defence status to combat immediate overload of oxidative stress caused by methotrexate compared to zinc oxide concurrent therapy. The effect of zinc oxide pre-treatment was comparable with the hepatoprotective effect of silymarin, a proven herbal hepato protectant in alleviating methotrexate induced toxicity.
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    ThesisItemOpen Access
    PATHOMORPHOLOGICAL AND MOLECULAR EVALUATION OF METHOTREXATE (MTX) INDUCED TOXICITY AND ITS AMELIORATION BY ZINC OXIDE (ZnO) NANOPARTICLES
    (KARNATAKA VETERINARY, ANIMAL & FISHERIES SCIENCES UNIVERSITY, BIDAR, 2019-07) AKSHATA S. ANGADI; SUGUNA RAO
    The present study was pursued to evaluate the pathomorphological, biochemical and molecular changes in methotrexate induced toxicity and its amelioration by zinc oxide nanoparticles treatment in rats for 45 days. The study included normal control (Group I), methotrexate control (Group II), zinc oxide nanoparticle control (Group III), zinc oxide nanoparticles pre-treatment (Group-IV), ZnO NP concurrent treatment (Group-V) and silymarin treatment (Group-VI) groups. Toxicity was induced in rats by administration of methotrexate at the dose rate of 5 mg/kg bw IP for three consecutive days and zinc oxide nanoparticles were used at the dose rate of 50 mg/kg bw PO. The parameters assessed following the administration of methotrexate and zinc oxide nanoparticles in different groups included clinical signs, biochemical parameters, antioxidant enzymes, lipid peroxidation assay and gross and histopathology. Molecular study included immunohistochemical evaluation of p53 expression and relative quantification of p53 expression in liver by real time PCR. Methotrexate induced toxicity was characterized by necrosis and apoptosis. The pre-treatment and concurrent zinc oxide nanoparticles administration partially alleviated the methotrexate induced toxic effects. The pre-treatment of zinc oxide nanoparticles rendered better acquisition of overall defence status to combat immediate overload of oxidative stress caused by methotrexate compared to zinc oxide concurrent therapy. The effect of zinc oxide pre-treatment was comparable with the hepatoprotective effect of silymarin, a proven herbal hepato protectant in alleviating methotrexate induced toxicity.
  • Thumbnail Image
    ThesisItemOpen Access
    PATHOMORPHOLOGICAL AND MOLECULAR EVALUATION OF METHOTREXATE (MTX) INDUCED TOXICITY AND ITS AMELIORATION BY ZINC OXIDE (ZnO) NANOPARTICLES
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2019-07) AKSHATA S. ANGADI; SUGUNA RAO
    The present study was pursued to evaluate the pathomorphological, biochemical and molecular changes in methotrexate induced toxicity and its amelioration by zinc oxide nanoparticles treatment in rats for 45 days. The study included normal control (Group I), methotrexate control (Group II), zinc oxide nanoparticle control (Group III), zinc oxide nanoparticles pre-treatment (Group-IV), ZnO NP concurrent treatment (Group-V) and silymarin treatment (Group-VI) groups. Toxicity was induced in rats by administration of methotrexate at the dose rate of 5 mg/kg bw IP for three consecutive days and zinc oxide nanoparticles were used at the dose rate of 50 mg/kg bw PO. The parameters assessed following the administration of methotrexate and zinc oxide nanoparticles in different groups included clinical signs, biochemical parameters, antioxidant enzymes, lipid peroxidation assay and gross and histopathology. Molecular study included immunohistochemical evaluation of p53 expression and relative quantification of p53 expression in liver by real time PCR. Methotrexate induced toxicity was characterized by necrosis and apoptosis. The pre-treatment and concurrent zinc oxide nanoparticles administration partially alleviated the methotrexate induced toxic effects. The pre-treatment of zinc oxide nanoparticles rendered better acquisition of overall defence status to combat immediate overload of oxidative stress caused by methotrexate compared to zinc oxide concurrent therapy. The effect of zinc oxide pre-treatment was comparable with the hepatoprotective effect of silymarin, a proven herbal hepato protectant in alleviating methotrexate induced toxicity