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    ThesisItemOpen Access
    MOLECULAR AND GENOMIC CHARACTERIZATION OF VIBRIO VULNIFICUS ASSOCIATED WITH MOLLUSCAN SHELLFISH
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018) SANGEETHA, M.S.,; M.N.VENUGOPAL
    V. vulnificusis a halophilic bacteria autochthonous to the marine environment and considered as important seafood-borne pathogen associated with molluscan shellfish. To ensure the safety of seafood and to initiate effective control measures to reduce the risk of V. vulnificus infection, we have analyzed it’s prevalence in seafood harvested along Mangalore coast using conventional and molecular approaches. A total of 132 seafood samples comprising of 103 clams (Meretrix meretrix), 22 oysters (Crassostrea madrasensis) and 7 green mussels (Perna viridis) were collected and analysed periodically (April 2014 to May 2016) for the presence of V. vulnificus. Among 103 clam samples, only 67 samples were found positive for V. vulnificus. A total of 105 V. vulnificus isolates were confirmed phenotypically and 10 were confirmed genotypically, which are all from clam samples. Nethravathy estuary samples harbored high counts of V. vulnificus compared to all other sampling sources. All the isolates belonged to biotype 1, genotype B and vcgC type based on different typing studies. All the isolates are susceptible to one or the other antibiotic studied and none of the isolates possessed the resistant genes tested. Different plate assaystudies for virulence activities revealed that except lecithinase activity, all the isolates given positive results for protease activity, lipase activity and hemolytic activity. PCR anlaysis of the different virulence genes revealed that all the isolates were found to possess all the virulence genes analysed. The presence of type three secretion system effector proteins was analysed and found that all the genes testedwere present in V. vulnificus isolates harvested from Mangalore coast. The overall results indicate that the seafood harvested along Mangalore coast known to contain potentially pathogenic V. vulnificus and suggest that there is a need for adequate consumer protection measures
  • Thumbnail Image
    ThesisItemOpen Access
    MOLECULAR AND GENOMIC CHARACTERIZATION OF VIBRIO VULNIFICUS ASSOCIATED WITH MOLLUSCAN SHELLFISH
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018) SANGEETHA, M.S.,; M.N.VENUGOPAL
    V. vulnificusis a halophilic bacteria autochthonous to the marine environment and considered as important seafood-borne pathogen associated with molluscan shellfish. To ensure the safety of seafood and to initiate effective control measures to reduce the risk of V. vulnificus infection, we have analyzed it’s prevalence in seafood harvested along Mangalore coast using conventional and molecular approaches. A total of 132 seafood samples comprising of 103 clams (Meretrix meretrix), 22 oysters (Crassostrea madrasensis) and 7 green mussels (Perna viridis) were collected and analysed periodically (April 2014 to May 2016) for the presence of V. vulnificus. Among 103 clam samples, only 67 samples were found positive for V. vulnificus. A total of 105 V. vulnificus isolates were confirmed phenotypically and 10 were confirmed genotypically, which are all from clam samples. Nethravathy estuary samples harbored high counts of V. vulnificus compared to all other sampling sources. All the isolates belonged to biotype 1, genotype B and vcgC type based on different typing studies. All the isolates are susceptible to one or the other antibiotic studied and none of the isolates possessed the resistant genes tested. Different plate assaystudies for virulence activities revealed that except lecithinase activity, all the isolates given positive results for protease activity, lipase activity and hemolytic activity. PCR anlaysis of the different virulence genes revealed that all the isolates were found to possess all the virulence genes analysed. The presence of type three secretion system effector proteins was analysed and found that all the genes testedwere present in V. vulnificus isolates harvested from Mangalore coast. The overall results indicate that the seafood harvested along Mangalore coast known to contain potentially pathogenic V. vulnificus and suggest that there is a need for adequate consumer protection measures.