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M. Sc. Dissertations

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Author: Sunil Kumar × End date: 2007 × Start date: 2007 ×
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    ThesisItemOpen Access
    Studies on myb gene expression in Indian mustard (Brassica juncea L. Coss. & Czern) under salt stress
    (CCSHAU, 2007) Sunil Kumar; Yadav, R.C.
    The investigation was carried out to study the myb expression under different levels of salinity in Brassica juncea cv. CS52 in the Department of Biotechnology & Molecular Biology, CCS Haryana Agricultural, Hisar during the year 2006 and 2007. The myb gene expression was checked by RT-PCR approach. Primers BjMYBR-1 and BjMYBR-2 were designed from the conserved DNA binding domain of Atmyb2 by analyzing this gene sequence with other myb gene sequence available in database using ClustalW programme. The expression of Bjmyb1 became detectable within 15 minutes of salinity exposure, which increased and was maximum in 30 minutes of salinity treatment. Thereafter it declined and stabilized in 2 hr of salinity treatment. The expression was not affected by the different concentrations (100 mM, 200 mM, 300 mM and 400 mM) of NaCl used. Sequencing of amplified cDNA fragment revealed a 176 bp sequence. ClustalW analysis of Bjmyb1 with other eight myb genes from different organisms available in database showed its homology with Atmyb2 and Chmyb2.
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    ThesisItemOpen Access
    Management of mungbean yellow mosaic virus in mothbean [Vigna aconitifolia (Jacq) marechal]
    (CCSHAU, 2007) Sunil Kumar; Gupta, P.P.
    Mothbean is a major commercial crop of hot arid regions of our country. It is affected by several fungal, bacterial and viral diseases. Among these mungbean yellow mosaic virus (MYMV) disease is the most important. Out of 62 genotypes screened against MYMV disease under field conditions and seventeen among them screened under green house conditions only 12 genotypes including one promising resistant check viz., RMM-47-2, RMM-44, GMO-4, RMO-257, RMB-54, CZM-90, HM-61, CZM-12, RMO-435, GMO-13, GMO-9915, CAZRI Moth-1 were found moderately resistant. Second week of June for sowing and spacing 45 cm were optimum in reducing the incidence of MYMV disease and whitefly population. The yield was also maximum (10.33 q/ha) at this conditions. There was positive correlation between disease incidence and whitefly population and yield was negatively correlated with disease incidence and whitefly population. The correlation of disease incidence with weather parameters were found non-significant. Application of phosphorus and potassium (40 kg/ha each) alone reduces the disease incidence from 47.8% (in control, no application of NPK) to 17.8% and 18.3% respectively. Highest reduction in MYMV disease incidence (70.61%), minimum whitefly population and maximum grain yield with respect to 20 kg/ha nitrogen application alone was recorded when N20P40K40 kg/ha were used. The botanical Azadirachtin (Fortune Aza, 5%) was most effective than other botanicals in reducing the MYMV disease incidence (69.43%) and increasing the grain yield (84.56%). This treatment was statistically at par with the application of Endosulfan (0.07% a.i.). Neem oil (5%) was found better next to the Azadiractin in reducing the disease incidence.