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Author: Parveen Kumari × Start date: 2016 × Type: Thesis ×
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    ThesisItemOpen Access
    Phytochemical composition and antioxidant activity of leaves, bark and seeds of chinese rain tree (Koelreuteria elegans)
    (CCSHAU, 2016) Parveen Kumari; Khabiruddin, M.
    The present study was undertaken for phytochemical composition and antioxidant activity of bark, leaves and seeds of Chinese rain tree (Koelreuteria elegans). The plant materials were collected from the university campus CCS, Haryana Agricultural University, Hisar. After cleaning, these were dried in shade and ground to fine powdered form. Ash content, crude fibre, crude protein, carbohydrates, energy value and minerals (Zn, Cu, Mn, Fe), analysed in the seeds, were found to be 1.3±0.1 %, 10.3±0.6 %, 19.5±0.6 %, 36.8±1.5 %, 627.3±6.6 KJ/100g respectively and Zn content was found to be 55.0±1.4 ppm, Cu content was 175.0±3.5 ppm, Mn content was 606.0±28.3 ppm and Fe content was 519.0±16.5 ppm. Moisture content and oil content were 7.1±0.2 % and 25.0±0.2 % respectively. Peroxide value, iodine value, saponification value, unsaponifiable matter and free fatty acids in the seed oil, were found to be 1.8±0.1 meq/kg, 90.9±1.2 g of I2/100 g oil, 198.1±0.2 mg/g KOH, 4.3±0.1 % and 1.2±0.02 as % oleic acid. Total phenols, total carotenoids and total tocopherol in the oil were found to be 2.8±0.04 mg GAE/g, 15.1±0.3 mg/kg and 34.7±1.8 mg/100g respectively. Total flavonoids were in traces. The fatty acid composition of the oil was found as palmitic acid (4.9±0.2 %), oleic acid (22.8±0.1 %), linoleic acid (9.7±0.2 %), arachidic acid (54.8±0.2 %) and behanic acid (1.3±0.1 %). Total phenols in methanol extract of bark, leaves and seed cake were found to be 99.3±1.2 mg GAE/g, 109.1±2.5 mg GAE/g and 60.0±0.1 mg GAE/g respectively. Total flavonoids in the methanol extract of bark, leaves and cake were found to be 26.9±0.4 mg CAE/g, 36.3±0.2 mg CAE/g and 10.0±0.06 mg CAE/g respectively. Antioxidant activity of the extracts were determined by DPPH free radical scavenging method and was found to be 96 % at conc. of 0.06 mg/ml in the bark extract and 95 % at conc. of 0.07 mg/ml in the leaf extract and 93 % at conc. of 0.07 mg/ml in the seed cake extract respectively. EC50 was 0.023±0.002 mg/ml for bark extract, 0.022±0.002 mg/ml for leaf extract and 0.025±0.01 mg/ml for the seed cake extract respectively.