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Author: Jyoti × End date: 2009 × Start date: 2009 ×
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    ThesisItemOpen Access
    Analysis of diversity for micronutrient content and DNA fingerprinting in Greengram [Vigna radiata (L.) Wilczek]
    (2009) Jyoti; Yadav, Neelam
    Vigna radiata (L.) Wilczek, commonly called mungbean or greengram is the third most important pulse crop of India occupying nearly 3 million hectare. Mungbean provides inexpensive, low flatulence and easily digestible protein available in several edible forms that complements staple rice diet in Asia. India is the primary greengram producer and contributes about 75% of world production. Iron and Zinc are important micronutrients for human health whose deficiency causes anemia and malfunctioning of immune system. Commercial cultivars contain low levels of iron and zinc and it is important to assess genetic -IIvariability in available germplasm for improving micronutrient content in commercial cultivars. The present study was undertaken to study RAPD polymorphism among 16 mungbean genotypes using 33 decamer primers in PCR reaction. A total of 148 amplified bands were produced out of which 142 were polymorphic and 6 were monomorphic. For the genotypes studied, upto bands were produced with an average of 6.16 bands per primer. The size of amplified bands ranged from 150-3000 bp. Some unique bands were also produced, which could be used to distinguish these genotypes. The similarity coefficients between different genotypes ranged from 0.40-0.89 with an average similarity value of 0.65. At an arbitrary cut-off at 60 per cent similarity level on a dendrogram, the mungbean accessions were categorized into two major clusters. Satya and Asha were found to genetically similar. ML776 showed high iron and zinc content while Satya was poor in iron as well as zinc content. OPB7 primer showed 900 bp amplified product in ML776 that was found absent in Satya. These two genotypes can be used in developing population for linkage mapping. The results indicated that RAPD markers were efficient for identification of Vigna radiata genotypes and for determination of the genetic relationships among them.