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M. Sc. Dissertations

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2006 - 20073
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Subject: Zoology × End date: 2007 ×
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Now showing 1 - 3 of 3
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    ThesisItemOpen Access
    Sequencing of Mx gene in an indian major carp
    (CCSHAU, 2006) Sharma, Parvati; Sihag, R.C.
    Mx gene is inducible by type I interferon and is involved in antiviral defenses. Interferons are pleiotropic glycoproteins belonging to a large family of Cytokines. The type I interferon constitute the first line of defence against viral infection in the vertebrate innate immune system. Mx gene inducible by type I interferon, natural and synthetic double stranded RNA (ds RNA) and unmethylated bacterial CpG motif, both in fish and mammalian hosts. Mx expression was observed in fish after viral infection (i.e. IPNV, VHSV, ISAV), although the antiviral role of Mx proteins in fish remains unclear. Mx gene has been cloned and sequenced in many fishes including gold fish and grass carp of family Cyprinidae. The aim of the present study was to standardize the RT-PCR protocol for the expression of Mx gene in an Indian major carp, rohu (Labeo rohita). Mx response was measured in rohu injected with the synthetic double stranded polyribonucleotide Poly I: C (500 μg/fish) and the control fish was injected with PBS alone. Total RNA was extracted from liver of acclimatized fish after 3 days of post-injection. It was transcribed to cDNA and amplification of cDNA was done by RT-PCR. The sequence was obtained from purified gel product. The blasts search was done for confirmation of Mx gene in rohu. It was found 99% matching with grass carp Mx and 98% matching with gold fish Mx3 belonging to family Cyprinidae. These results confirm the presence of Mx gene expression in an Indian major carp, rohu. The obtained partial sequence can be used for taxonomic and phylogenetic purposes.
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    ThesisItemOpen Access
    Floral biology, mellitophily and pollination ecology of tulsi (Ocimum sanctum Linn.)
    (CCSHAU, 2007) Monika; Sihag, R.C
    An abstract of the dissertation submitted to the Chudhary Charan Singh Haryana Agricultural University, Hisar in partial fulfillment of the requirements for the degree of Masters of Science in Zoology. Medicinal and aromatic plants have become an important part of daily life world over despite significant progress in modern medical and pharmaceutical research (Singh and Somadey, 2005). Tulsi (Ocimum sanctum Linn.) is worshiped for its role in maintaining general peace in the house. This is one of the most important medicinal and aromatic plants of the biosphere. The main chemical ingredients in this plant are eugenol, carvacrol, methyl eugenol and caryophyllene. The study was made on floral structure, floral phenology, mellitophily, pollination ecology and the role of different modes of pollination in seed yield of tulsi. For the study of flower structure freshly opened flowers were taken and studied under dissecting microscope and revealed the protandrous condition of the flower. Fourteen insect species were found to visit the blossoms of tulsi crop. Out of these, six species were the main pollinators i.e. Apis .dorsata, Apis .mellifera, Apis .florea, Mellisodes sp., Halictus sp., Nomia sp. On the basis of foraging rate, A .dorsata was found to be the fastest forager among the pollinators and Mellisodes sp. was the slowest .Abundance of insect visitors was maximum during peak flowering period. Among the hymenopterous insects, A .dorsata was most abundant followed by Nomia sp., Mellisodes sp., A .mellifera, Halictus sp. and then A .florea. Among all insect pollinators, A .dorsata remained active for longest duration followed by Mellisodes sp., Nomia sp., A .mellifera, Halictus sp. and A .florea, respectively. Bee activity followed the same pattern as temperature did; however, humidity followed a reverse trend. Pollinating efficiency of the pollinators derived on the basis of their abundance, foraging rate and activity duration revealed that A. dorsata was the most efficient pollinator of this crop. Differences in different pollination treatments (i.e. insect, open, wind and self-pollination) on different yield parameters (e.g. number of seed set per inflorescence, yield per plant and per plot, test weight of seeds and percent germination) were significant. Seed setting was found to be highest in insect pollination treatment (1045.66 seeds/inflorescence) followed by 565 seeds/inflorescence in open pollination. However, seed set was found to be very low in other two treatments i.e. wind pollination and self-pollination. Similar results were observed in case of yield per plant and yield per plot. Test weight and percent germination was found to be similar for the three pollination treatments i.e. for insect pollination, open pollination and wind pollination respectively but in case of selfpollination it was near about half of the former three due to deformed and under sized seeds in the latter treatment. Pollination experiments clearly revealed that tulsi flowers are highly benefited from insect visits .Its yield parameters showed that it is a cross-pollinated and entomophilous plant .In general, the yield in this plant is pollinator limited i.e. there is scarcity of pollinators to achieve the maximum goal as is evident by yield data in insect pollination treatment. The structure shape and fertility status of the flower clearly revealed that it has bee pollinated flowers and melittophily pollination syndrome existed in tulsi.
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    ThesisItemOpen Access
    Ecology and management of tetranychus urticae koch on okra, abelmoschus esculentus L.
    (CCSHAU, 2007) Geroh, Monika; Gulati, Rachna
    Studies on seasonal incidence of T. urticae revealed two peaks in its population on okra during first fortnight of June (25.69 mites/ sq. cm leaf) and during first fortnight of August (22.50 mites/ sq. cm leaf). Predatory beetle S. punctillum also showed two peaks; in the first fortnight of July (0.56 beetle/leaf) and second peak coincided with peak in T. urticae population, in the first fortnight of August (0.48 beetle/leaf). Strata wise, middle strata harboured significantly more number of mites and beetles followed by top and bottom strata. A significant positive correlation(r =0.88) was observed between mite and beetle population during the present study. With abiotic factors, T. urticae and S. punctillum population showed significant positive correlation with maximum and minimum temperature. Under in vitro conditions, direct spray bioassay showed smaller LC50 and LT50 values as compared to treated food bioassay. Under in vivo, P. pinnata oil (6.24%) was most effective which caused 82.3 percent reduction in T. urticae population followed by E. globulus oil @ 4.60% (76.1%), P. pinnata seed extract @ 9.68% (60.5%) and E. globulus leaf extract @ 3.92% (36.68%). All extracts/ oil were found safe to mite predator, S. punctillum.