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Subject: Vegetable Science × Author: Jammihal, Shivanand N. × End date: 2009 × Start date: 2006 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Effect of explants and media combinations on plant regeneration in different genotypes of tomato
    (CCSHAU, 2007) Jammihal, Shivanand N.; Rana, M.K.
    The investigation was carried out on “Effect of explants and media combinations on plant regeneration in different genotypes of tomato” in the Department of Biotechnology and Molecular Biology, Chaudhary Charan Singh Haryana Agricultural University, Hisar during the year 2006 and 2007. Development of protocols for in vitro selection would provide new advances for the production of stress tolerant cultivars in tomato. The seedling of tomato cultivars Hisar Lalima (Sel.18) and Hisar Anmol (H-24) were used as planting material in the present studies. Hypocotyls and cotyledons were used as the source of explants, which were obtained from 25 to 30 days old seedlings grown under aseptic conditions. These explants were cultured on MS medium supplemented with different concentrations of BAP and kinetin in combination with IAA and IBA 0.2 mg/l. Two media, i.e., MS basal fortified with BAP 2.5 mg/l + IAA 0.2 mg/l and kinetin 2.5 mg/l + IAA 0.2 mg/l showed the maximum shoot formation and number of shoots per explant among all 24 different media combinations. The cotyledon explants were found to be more efficient in producing shoot formation. Among 24 treatments, the MS basal containing BAP 2.5 mg/l + IAA 0.2 mg/l for hypocotyls and MS basal supplemented with kinetin 2.5 mg/l + IAA 0.2 mg/l for cotyledons was found the best for days to shoot formation, percent shoot formation and number of shoots per explant. The regenerated shoots produced roots (100%) in both the cultivars Hisar Lalima and Hisar Anmol on MS medium containing IAA 0.2 mg/l, and were successfully hardened and transferred to the screen-house conditions.