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M. Sc. Dissertations

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  • ThesisItemOpen Access
    Effect of priming treatments on seed quality in guar (Cyamopsis tetragonoloba)
    (CCSHAU, 2010) Arun Kumar; Kharb, R.P.S.
    The clusterbean (guar) is an important arid legume crop and recognized as an industrial crop due to presence of galectomannan (gum) content in the seed. The present investigation was carried out at the department of Seed Science and Technology, CCS Haryana Agricultural University, Hisar. The material comprised of two seed-lots (good quality=L1 and marginal=L2) of each of two popular guar varieties (HG-365 and HG-563). The priming treatments (hydropriming, halopriming, osmopriming, biopriming)were applied to all the variety-lot combinations to assess their effect on physiological and field parameters and relative storability. Pre-sowing seed invigoration treatments T1 (H-D), T6 (KNO3, 0.5%) and T8 (Pseudomonas culture) enhanced germination (6.66-12.66%) in good quality seed-lot of variety HG-365, whereas, the treatment T3 (GA3, 50ppm), T5 (PEG-6000, -5bar), T7 (Rhizobium culture) improved the germination percentage (6.67-11.00%) considerably over control in good quality seed-lot of variety HG-563. The treatments T3, T5, T1, and T6 enhanced total seedling emergence (3.83-7.5%) in good qualit y lot of HG-365, whereas, the treatment T1 and T3 enhanced SET percentage (6.00-6.33%) over control in marginal seed lot of HG-563. Similar trend was found for FEI and MET for both the varieties. The SET was found significant ly and positively correlated with SG, vigour indices (VI-I and VI-II), AA, and FEI and negatively with MET. These parameters can be used as reliable predictors of total seedling emergence potential and seedling establishment. The treatments T3 (GA3, 50ppm), followed by T1 (H-D), and T8 (Pseudomonas culture) enhanced the seed yield per plant, commonly in seed lots of both the varieties. The characters pods/plant followed by pod length was found associated with seed/plant. Based on the AA and EC test, the treatments T1, T2, T3, and T5 were found commonly most effective for enhancing the relative storability particularly in good quality seed lot of the varieties. Overall, the treatment T3 (GA3, 50ppm) was found commonly superior in all variety-lot combinations, for enhancing standard germination, final field emergence and reducing the mean emergence time (MET).
  • ThesisItemOpen Access
    Studies on seed viability and vigour in naturally aged seeds of coriander (Coriandrum sativum L.)
    (CCSHAU, 2010) Vipesh Kumar; Verma, Sher Singh
    In the present investigation three seed lots of eight varieties/genotypes of coriander viz., DH-224, DH-226, DH-228, DH-242, DH-259, DH-294, DH-296 and Hisar Anand, were subjected for different viability and vigour parameters with the objectives to study the effect of natural ageing on seed quality parameters. All the seed lots were analyzed for standard germination test (%), root length (cm), shoot length (cm), seedling dry weight (mg), vigour index-I, vigour index-II, accelerated ageing test (%), seed density (g/cc), electrical conductivity (S/cm/seed) of seed leachates, dehydrogenase activity test (O D g-1 mL-1), field emergence index and field establishment (%) and, the association of various seed quality parameters with field emergence rate index were determined. Results revealed that all the varieties/genotypes showed the germination percentage above the Minimum Seed Certification Standards (60%) in Lot-A and Lot-B. It was also observed that for standard germination test (%), root length (cm), shoot length (cm), seedling dry weight (mg), vigour index-I, vigour index-II, accelerated ageing test (%), seed density (g/cc), dehydrogenase activity test (O D g-1 mL-1), field emergence index and field establishment (%) decreased significantly and progressively as the ageing period increased. The electrical conductivity was found negatively and significantly correlated with all seed viability and vigour parameters. Results also revealed that viability and vigour of seeds declined with faster rate in Lot-C (2½ years old seed). Among all the varieties/genotypes, genotypes DH-224 and DH-228 were found most promising for various parameters of viability and vigour. The standard germination test, vigour index-I, accelerated ageing test and vigour index-II emerged as reliable predictors of field emergence rate index.
  • ThesisItemOpen Access
    Effect of priming treatments on seed quality in greengram. (Vigna radiata L.)
    (CCSHAU, 2010) Beniwal, Inderpal; Dhaiya, O.S.
    The greengram (mung) is an important arid pulse crop, its significance lies in crop rotation and inter cropping as well on account of their ability to enrich soil by fixing atmospheric nitrogen and healthy diet. The material comprised of two seed lots (good quality=L1 and marginal = L2) of each of two popular varieties of greengram (Asha and Muskan). The different priming treatment (Hydration 14-16h, 2% CaCl2, 50 ppm GA3, hydration-dehydration+dry dressing with thiram @ 0.25% and 0.5% KNO3) were applied to all the variety lots and combination to assess their effect on seed quality. The significant mean sum of squares due to variety, lots and treatments and their interaction indicated the presence of sufficient amount of variation for most of the parameters studied. Lot L2 seeds showed better improvement over lot L1 for standard germination, shoot length, dry weight, vigour index-II, DHA, 50% flowering, days to maturity and yield. Whereas lot L1 showed better improvement in root length, vigour index-I, EC, speed of emeregence, seedling establishment and final plant stand. The variety Asha showed higher improvement in standard germination, shoot length, root length,dry weight, vogour index-I, vigour index-II, DHA, 50% flowering, days to maturity , speed of emeregence and yield. Muskan showed higher improvement in EC, DHA, seedling establishment, final plant stand and 50% flowering. Among various treatments hydration-dehydration + dry dresing with thiran @ 0.25% were found most effective for improving shoot length, dry weight, vigour index-I , vigour index-II, DHA, speed of emeregence, seedling establishment, final plant stand, 50% flowering, days to maturity and yield followed by hydration-dehydration. Therefore, it is concluded from this study that the germination level of marginal/ sub standard seed lots in greengram can be improved by using presowing seed treatments.
  • ThesisItemOpen Access
    Studies on seed quality evaluation in barley (Hordeum vulgare L.)
    (CCSHAU, 2011) Vikas Singh; Kashyap, R.K.
    The twenty genotypes of barley were evaluated for seed quality by various viability, vigour tests with three replications, and observations were recorded on standard germination, seedling length, seedling dry weight, seed weight, seed density, vigour index-I, Vigour Index-II, accelerated ageing test, electrical conductivity, tetrazolium test, dehydrogenase activity test, pH exudates test, field emergence index, seedling establishment and mean emergence time .Significant mean squares values showed the wide variation among the genotypes for all studied parameters. Among all the genotypes BH 393, BH 942 and RD 2552 showed the top rank in almost all parameters. On the contrary, PL 751 and BH 932 genotypes found to be inferior for almost all the viability and vigour parameters. All the parameters were significantly positively correlated with standard germination except seed weight, seed density, pH exudates test and mean emergence time whereas field emergence also showed the positive correlation with all parameters except seed density, pH exudates test and mean emergence time. The electrical conductivity was negatively correlated with standard germination and field emergence. Tetrazolium test followed by vigour index-I showed highest reliability for prediction of standard germination whereas vigour index-I followed by tetrazolium showed the highest reliability for prediction of field emergence. Correlation coefficient analysis and regression analysis revealed that vigour and viability parameters viz., tetrazolium test, Dehydrogenase activity test, vigour indices, electrical conductivity, accelerated ageing, and seedling length can be used as reliable predictors of standard germination and field emergence in barley. The tetrazolium test and vigour index-I should be most preferred as they are not only quick and cost effective but also easy to perform.
  • ThesisItemOpen Access
    Studies on seed viability and vigour in pearl millet [Pennisetum glaucum (L.)R. Br]
    (CCSHAU, 2011) Ananda, H.A.; Deswal, D.P.
    Twenty genotypes of pearl millet were evaluated for seed quality by various viability, vigour tests with three replications and observations were recorded on standard germination, seedling length, seedling dry weight, seed weight, seed density, vigour index-I, Vigour Index-II, accelerated ageing test, electrical conductivity, tetrazolium test, dehydrogenase activity test, pH exudates test, field emergence index, seedling establishment and mean emergence time .Significant mean squares values showed the wide variation among the genotypes for all studied parameters. The hybrids were superior for most of the vigour and viability parameters than their parental lines. The hybrids namely HHB 216 and HHB 226 ranked at top in almost all parameters. Among ‘R’, ‘A’, and ‘B’ lines HTP 3/13, HMS 7A and HMS 37B respectively were found superior, based on majority of viability and vigour tests.on the other hand ‘A’ line ICMA 97111 and ICMA 94555 has shown lowest rank in most of parameters. All the parameters were significantly positively correlated with standard germination except seedling dry weight, seed density and pH exudates test whereas, field emergence also showed the positive correlation with all parameters except seedling dry weight and pH exudates test. The electrical conductivity was negatively correlated with standard germination and field emergence. Tetrazolium test followed by vigour index-II showed highest reliability for prediction of standard germination whereas standard germination followed by tetrazolium showed the highest reliability for prediction of field emergence. Correlation coefficient analysis and regression analysis revealed that vigour and viability parameters viz., tetrazolium test, Dehydrogenase activity test, vigour indices, electrical conductivity and accelerated ageing, can be used as reliable predictors of standard germination and field emergence in pearl millet. The tetrazolium test and electrical conductivity test should be most preferred as they are not only quick and cost effective but also easy to perform.