Thumbnail Image

M. Sc. Dissertations

Browse

Reset filters
Subject: Seed Science and Technology × Author: Jain, Umesh Kumar × End date: 2009 × Start date: 2009 ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    Varietal characterization based on morphological and biochemical techniques in rice (Oryza sativa L.)
    (CCSHAU, 2009) Jain, Umesh Kumar; Verma, S.S.
    In the present study, fifteen varieties of rice (Oryza sativa L.) were characterized based on twelve morphological descriptors at Research Farm of Rice Research Station, Kaul and biochemical tests in the laboratories of Department of Seed Science and Technology, CCS Haryana Agricultural University, Hisar during 2007-08. The study revealed that all the varieties of rice were identified on the basis of morphological parameters viz. leaf colour, plant habit, flag leaf angle, node base colour, awning, colour of lemma & palea, length of awning, 100-seed weight (g) and 50% days of flowering, response to different chemicals (Phenols, Modified phenols with Cu++ or Fe++ or Na2CO3, NaOH and FeSO4). The study revealed that leaf pigmentation, hairiness of leaf sheath and leaf blade, secondary branching of panicle and KOH-bleach test were not found much helpful in distinguishing the rice varieties. It was also observed that individual morphological parameter or chemical test was not able to distinguish all the varieties. However, the combinations of different morphological and chemical tests were found useful in identification of individual variety. The results also revealed that Tris-HCl buffer soluble seed storage proteins of fifteen rice varieties were analyzed using SDS-PAGE and found the resultant banding patterns enabled identification of all varieties confirming the distinguishing power of this procedure for varietal identification of rice. Hence, it could be used as a powerful tool to identify every genotype in a short period of time. It is, therefore, concluded that the morphological parameters and biochemical tests could be used for genetic purity testing in rice.