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20101
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Subject: Molecular Biology and Biotechnology × Author: Jogender Singh × End date: 2010 × Type: Thesis ×
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    ThesisItemOpen Access
    Development and molecular marker analysis of F1 and/or backcross plants from crosses between aerobic and high yielding rice cultivars
    (CCSHAU, 2010) Jogender Singh; Jain, R.K.
    The present investigation has been undertaken to: (i) carry out field evaluation of various Basmati (Pusa1121, HBC19 and Pusa Basmati 1460), indica (PAU201, HKR47) and aerobic (MAS-ARB25, MASARB868, MAS25, MAS26 and MAS109,) rice varieties for various agronomic traits under submerged field conditions, (ii) develop F1 and/or backcross plants from crosses between aerobic and high yielding rice cultivars, and (ii) assess the molecular status of F1 and parental rice genotypes for some markers linked with the root traits, abiotic stress tolerance and BAD2 aroma gene. Aerobic rice varieties had small panicle length and low yield per plant compared to Basmati and high yielding indica rice varieties when grown under submerged cultivations in the field. A DNA fingerprinting database of ten parental genotypes has been prepared using 29 SSR markers and a BAD2 aroma gene specific marker. High level of polymorphism was observed among the aerobic, Basmati and indica rice varieties. A total of 117 alleles were detected at 30 loci. Number of alleles per locus ranged from 2 to 6 with an average of 3.9 alleles per locus. The overall size of PCR products amplified range from 70-585 bp. The molecular size difference between the smallest and largest allele at a locus varied from 2 to 328. The polymorphism information content (PIC) values, which are indicative of genetic diversity, ranged from 0.180 to 0.820 with an overall average of 0.616 per locus. The NTSYS-PC UPGMA tree cluster as well as PCA analysis clearly grouped the 10 rice genotypes into two major groups one with three Basmati rice cultivars and other with aerobic as well as indica rice genotypes. Polymorphism for seven microsatellite markers (RM205, RM206, RM242, RM440, RM447, RM562 and RM7075) and BAD2 aroma gene specific (Bradbury et al. 2005) markers with a base difference of >30 bp in the amplified products, could be clearly visualized on 2.5% w/v agarose gels. The two F1 plants HBC19 x MAS26 and Pusa1121 x MAS25 showed lower levels of seed setting (8.0 and 20.6%, respectively) that too only in end of October and starting of November. No backcrossed seed was obtained. A total of 15 crosses were made between aerobic and high yielding indica /Basmati rice varieties; crossed seed were recovered from 11 crosses. Seven plants obtained from the HKR47 x MAS26 and MASARB25 x Pusa Basmati crosses were confirmed as F1 hybrids with the help of SSR RM242 and BAD2 aroma gene specific markers.