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M. Sc. Dissertations

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20111
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Subject: Microbiology × Author: Aggarwal, Vishal Kumar × End date: 2016 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Growth promotion activity and molecular diversity of root endophytic bacteria from legume and non-legume plants
    (CCSHAU, 2011) Aggarwal, Vishal Kumar; Pathak, D.V.
    Large numbers of bacterial endophytes have been reported from different plant tissues. These endophytes exhibit many beneficial characters for plant growth promotion and nitrogen fixation. These endophytes may perform similar or different functions in different plants and in different tissues of a plant. Therefore, a total of 90 root endophytic bacteria were isolated from roots of different crops chickpea (12), pea (15), wheat (14), lucerne (24) and oat (25) after proper surface sterilization. Studies of different morphological characters showed that 52 isolates were gram positive and 38 isolates were gram negative. Almost all the 50 gram positive isolates were spore formers with terminal or central spore. All the endophytes were screened for the presence of beneficial characters, and found that 62.3% endophytic bacteria were root growth promoter, 47.8% endophytic bacteria were phosphate solubilizers, 67.7% root endophytic bacteria were ammonia producers and 20% root endophytic bacteria were organic acid producers. Molecular studies on diversity of selected 43 endophytic isolates showed that in chickpea roots five biotypes of endophytic bacteria were present, whereas four in lucerne and wheat and three in pea and oat roots were present. Seven biotypes in legumes and six in non-legumes were present. Overall 10 biotypes of bacterial endophytes were present in roots of legumes and non-legumes being grown under CCS HAU farm. Selected 40 root endophytes were screened under pot culture conditions using chickpea as test host. Inoculation of root endophytes promoted the plant growth and nitrogen fixation of chickpea and best isolates were LRE3, ORE3A, CRE13 and CRE1.