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M. Sc. Dissertations

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20102
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Subject: Horticulture × Author: Datta, Koushik × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Tissue culture studies in gladiolus (Gladiolus x grandiflorus L.) varieties
    (CCSHAU, 2010) Datta, Koushik; Rana, G.S.
    The present investigation was carried out to optimized regeneration protocol in gladiolus varieties. Corm apical bud, corm axillary bud and cormel tip explants were used for shoot regeneration on different media supplemented with different plant growth regulators. Response for shoot regeneration was observed within four weeks on all the media tested for different explants. Cormel tips and corm apical bud explants gave best shoot regeneration (55.6 and 54.4 per cent respectively) on MS medium supplemented with BAP (4.0 mg/l) and kinetin (4.0 mg/l) and the maximum number of shoot was observed on the same media. The earliest shoot regeneration response was observed on 4th day on MS media supplemented with BAP (4.0 mg/l) and kinetin (3.0 mg/l). The longest shoot was recorded from MS medium supplemented with BAP (2.5 mg/l) and kinetin (2.5, 3.5 mg/l). Maximum (54.8 per cent) rooting of shoots, longest root and earliest (on 7th day) root formation was observed on ½ MS medium supplemented with 4.0 mg/l NAA. Healthy and strong rooting was observed on all the media tested within 15 days of culturing. Survival rate of regenerated plantlets was observed to be more than 80 per cent on transplantation to pots. The best performance of cv. Amsterdam was observed in respect to shoot and root length. Fidelio perform better with respect to minimum time taken for shoot regeneration and root formation. However, the cv. Friendship performed best in respect to per cent shoot regeneration and root formation. The survival per cent is best in cv. Fidelio both after 15 and 30 days of transfer to the potting mixture.
  • Thumbnail Image
    ThesisItemOpen Access
    Tissue culture studies in gladiolus (Gladiolus x grandiflorus L.) varieties
    (CCSHAU, 2010) Datta, Koushik; Rana, G. S.
    The present investigation was carried out to optimized regeneration protocol in gladiolus varieties. Corm apical bud, corm axillary bud and cormel tip explants were used for shoot regeneration on different media supplemented with different plant growth regulators. Response for shoot regeneration was observed within four weeks on all the media tested for different explants. Cormel tips and corm apical bud explants gave best shoot regeneration (55.6 and 54.4 per cent respectively) on MS medium supplemented with BAP (4.0 mg/l) and kinetin (4.0 mg/l) and the maximum number of shoot was observed on the same media. The earliest shoot regeneration response was observed on 4th day on MS media supplemented with BAP (4.0 mg/l) and kinetin (3.0 mg/l). The longest shoot was recorded from MS medium supplemented with BAP (2.5 mg/l) and kinetin (2.5, 3.5 mg/l). Maximum (54.8 per cent) rooting of shoots, longest root and earliest (on 7th day) root formation was observed on ½ MS medium supplemented with 4.0 mg/l NAA. Healthy and strong rooting was observed on all the media tested within 15 days of culturing. Survival rate of regenerated plantlets was observed to be more than 80 per cent on transplantation to pots. The best performance of cv. Amsterdam was observed in respect to shoot and root length. Fidelio perform better with respect to minimum time taken for shoot regeneration and root formation. However, the cv. Friendship performed best in respect to per cent shoot regeneration and root formation. The survival per cent is best in cv. Fidelio both after 15 and 30 days of transfer to the potting mixture.