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Subject: Veterinary Microbiology ×

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Now showing 1 - 9 of 27
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    ThesisItemOpen Access
    Characterization of Salmonella Callinarum Isolated From Natural Cases of Fowl- Typhoid
    (Birsa Agricultural University, Ranchi, 1989) Bhattacharya Asis; M.P. Singh
    There is no published report regarding the occurrence of S. gallinarum infection (Fowl typhoid) in part from Bihar state. However, the results of the present study indicate that there is incidence of S. gallinarum infection in poultry up to the extent of 4.06 per cent in Chotanagpur region of Bihar. Fowl typhoid is of extrems economic importance inpoultry in view of heavy mortality and egg borne transmission. It warrants the urgent need for systematic survey and investi gation to detect the infected birds and eliminate those birds from the flock. The incidence of the disease may go very high in future, unless a strict eradication programme is followed immediately. The present investigation suggests the need for the further research relevant to broadening the knowledge regarding the mechanism of enteropathogenicity of Salmonella gallinarum. There are good number of tests which can be used to diagnose fowl typhoid, but till any better diagnostic test is evolved, whole blood plate agglutination test is the only choice for detecting . gallinarum-pullorum infection in the field.
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    ThesisItemOpen Access
    Studies On Pox Diseases Of Sheep And Goats With Differential Diagnosis And Host
    (Birsa Agricultural University, Ranchi, 1989) Datta Subrata; J.P. Soman
    In the present study, using immunoperoxidase test, attempt was made to study the replicative nature and determine antigenic appearance in goat pox virus infected cells. The antigen was detected as early as 12 hour post-infection. The study indicated that the GPV multiplied in the cytoplasm of the cells as the antigens were exclusively detected only in the cytoplasm. In an attempt to develop a rapid and cheap immune diagnostic test for the differentiation of all three pox diseases, dot-immune binding assay was performed on common Whatman filter and Whatman cellulose acetate membrane filter papers. With HRP tagged goat pox immune globulins, positive reaction was observed in all the three pox viruses employed in the present study. The differential diagnosis was possible only with mono specific tagged immune globulins. Since dot immune binding assay is cheap, sensitive, easy to perform and takes only 3-4 hours in declaring the result, it is recommended that this technique as perfected in the present study can be employed for differential diagnosis of pox diseases of sheep and goats.
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    ThesisItemOpen Access
    STUDIES ON CONTAGIOUS ECTHYMA VIRUS WITH SPECIAL REFERENCE TO ITS CYTOPATHIC , PHYSICO- CHEMICAL AND IMMUNOGENIC CHARACTERISTICS
    (Birsa Agricultural University, 1986) Sinha, OM PRKASH; SOMAN, J.P.
    In the present study, using a local isolate of CEV described as "Ranchi" strain, attempts were made to find out the host range and to grow the virus in different cell cultures to study its cytopathogenicity and physico-chemical characteristics. Among other things, an immunological comparison was also made with SPV and GPV to select out a test for the differential diagnosis of pox infections of sheep and goats. The CEV was successfully cultivated in GK, SK and CHF cell cultures. The growth of the virus in cell cultures vas confirmed by inoculating the virus back to susceptible goats. The virus was found adapted by serial cultivation in GK cell cultures. The titre of the virus in this cell culture at 5th passage level was 5X106.5 TOID/ml, whereas it rose to 5x107.5 TOID50/m1 at 10th passage level. The granulation was observed to be the main characteristic sign of the OPE in GK and SK cell cultures, whereas rounding of cells was observed to be the most striking feature of the CPE in CEF cell culture. In stained preparations, eosinophilia (M&E) or metachromatic (KGG) inclusions with clear halos were observed in infected cells at 24 hours PI and onwards. The nuclear vacuolation as reported with GPV and SPV was not observed at any stage of infection. Similarly nuclear inclusions as reported with GPV were not noted with CEV. In some cells several organized small inclusions interspersed in the cytoplasm, in addition to the large mass, seemed to be another characteristic feature of the CEV. Tracts of infected
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    ThesisItemOpen Access
    CARRIER STATE OF SALMONELLA, PSEUDOMONAS AND PROTENS IN CHICKENS IN THE UNIVERSITY, STATE AND PROVATE POULTRY FARMS AROUND RANCHI CITY
    (Birsa Agricultural University, 1983) Prasad, KAMTA; Sharma, T.S.
    Salmonella, pseudomonas and Protein infection has assumed a considerable economic significance in all countries where the poultry industry is well established. Heavy mortality among chickens has been reported in many states of India. The present study on the incidence of the salmonello, Pasudomonas and Proteus has been undertaken. 1. The study included the poultry units of the Ranchi Veterinary Collega (R.V.C.), Ranchi and various private poultry farms around the Ranchi city. 2. For isolation and identification of Salmonella. Pseudomonas and Proteus, the standard bacteriological procedures recommended by Cowan and Steel (1970) were adopted. 3. A total of 900 samples including 557 samples from R.V.C. poultry units, Ranchi and 343 samples from private poultry farms were collected and processed. 4. The overall incidence of Salmanelle. Peeudomonas and Proteus were 2.11, 15.09, and 37.44 percent respectively. Their incidence in poultry units of Ranchi Veterinary College was 1.25, 12.02 and 30.16 percent respectively. 5. The incidence of Salmonella Pasudomonan and Proteus in private poultry farms vas 3.49 percent, 22.15 percent and 49.27 percent respectively. 6. The incidence of salmonella, Pseudomonas and Proteus in adult birds was 1.21 percent, 11.05 percent and 31.05 percent respectively whereas in young birds it was 3.69 percent, 24.24 percent and 48.48 percent respectively.
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    ThesisItemOpen Access
    IMMUNOLOGICAL STUDIES ON CERTAIN ASPECTS OF CAPRONE IMMUNE SYSTEMS IN PELATION TO MYCOPLASMA AGLACTIAE INFECTION
    (Birsa Agricultural University, 1986) Singh, TARAN; Tiwary, B.K.
    The presence study was undertaken to unmask some of the immunological parameters of goat not studied so far and secondly to elucidate the immune response of non-vaccinated (NVI) and vaccinated (VCI) goats to M. agalactiae organism. For vaccinating the goats formaline killed Al(OH)3 gel adsorbed M. agalactiae organisms was used. The results obtained are summarized as follows: Caprine Immune System: 1. Immunoglobulin concentration in sera and secretions of normal goats are not known. Using single radial immune diffusion test the mean concentrations in sera and tracheal washings of normal goats have been estimated and found to be 10.55 +0.807 mg ₂-1 and 1.045 ± 0.157 mg ml respectively. 2. Enumeration of T and B-cells in goats using rosette technique has not been attempted yet. By using E-rosette and EAC-rosette techniques probably for the first time in goat it has been screened out that the cattle erythrocytes have got the receptors for T lymphocyte of goat to form spontaneous B-rosette. It has been found that mean percentage of T and B-cells in normal goat peripheral blood lymphocytes are 23.0+ 0.50 and 27.33 0.49 percent, respectively. 3. Jerne-plaque technique has been employed to detect antibody forming cells/plaque forming cells (PFC) in peripheral blood and lymphoid organ of normal goats. Mean count of PFC per 106 lymphoid cell from peripheral blood, was found to be 75.60 Lymphoid cells.
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    ThesisItemOpen Access
    STUDIES ON CONTAGIOUS ECTHYMA VIRUS WITH SPECIAL REFERENCE TO ITS CYTOPATHIC , PHYSICO- CHEMICAL AND IMMUNOGENIC CHARACTERISTICS
    (Birsa Agricultural University, 1986) Sinha, OM PRKASH; SOMAN, J.P.
    In the present study, using a local isolate of CEV described as "Ranchi" strain, attempts were made to find out the host range and to grow the virus in different cell cultures to study its cytopathogenicity and physico-chemical characteristics. Among other things, an immunological comparison was also made with SPV and GPV to select out a test for the differential diagnosis of pox infections of sheep and goats. The CEV was successfully cultivated in GK, SK and CEP cell cultures. The growth of the virus in cell cultures vas confirmed by inoculating the virus back to susceptible goats. The virus was found adapted by serial cultivation in GK cell cultures. The titre of the virus in this cell culture at 5th passage level was 5x106.5 TOID/ml, whereas it rose to 5x107.5 TOID50/ml at 10th passage level. The granulation was observed to be the main characteristic sign of the CPE in GK and SK cell cultures, whereas rounding of cells was observed to be the most striking feature of the CPE in CEF cell culture. In stained preparations, eosinophilic (B) or metachromatic (MGG) inclusions with clear halos were observed in infected cells at 24 hours PI and onwards. The nuclear vacuolation as reported with GPV and SPV was not observed at any stage of infection. Similarly nuclear inclusions as reported with GPV were not noted with CEV. In some cells several organized small inclusions interspersed in the cytoplasm, in addition to the large mass, seemed to be another characteristic feature of the CEV. Tracts of infected cell in sheet as recorded after staining, indicate cell-to cell spread of the virus through cytoplasmic bridges.
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    ThesisItemOpen Access
    SEROLOGICAL COMPARISON AND IDENTIFICATION OF CAPRINE MYCOPLASMAS ASSOCIATED WITH PNEUMONIA
    (Birsa Agricultural University, 1987) Singh, LONGJAM CHAOBA; Singh, N.P.
    The study of cross reactivity among caprinsmycoplasman vis., Mycoplasma mycoides subsp. mycoides. M agalacties and arginini are very important for diagnostic as well as determination of specific, suitable and quick tests for identification purposes. Therefore, studies on the following serological tests were carried out for identification of mycoplasma. 1. In tube agglutination test, antisera raised against three species of caprine mycoplasmas gave a titre of 1 : 320 with their homologous antigens, while, in heterologous system anti-Mycoplasma mycoides subsp. mycoides serum agglutinated Magalactiae and I arvinini antigens upto 1:60 dilution. Similarly, anti-agalacticssarum agglutinated N. mycoldes subsp. mycaldosantiganupto 1150 dilution while M. arginini only upto 1:40. Anti-argininisorum gave a titre of 1:80 with mycoides subsp. mycoidasantion, whereas - agalactias gave titre only upto 1:40. These observations suggest more antigenic structural similarity between mycoides subsp. Bycoides and I agalactics as compared to –arginine. 2. The agar gel precipitation test revealed at least six precipitation lines among them, two were prominent in homologous system of mycoides subsp. mycoides. M. agalactiae and Harginini. In heterologous systems only three precipitation lines were observed among the different spacious of mycoplasmas.
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    ThesisItemOpen Access
    DEVELOPMENT OF SUITABLE IMMUNOPROPHYLACTIC AGENT AGAINST GOAT POX
    (Birsa Agricultural University, 1986) GOSWAMI, TAPAS KUMAR; SOMAN, J.P.
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    ThesisItemOpen Access
    SEROLOGICAL COMPARISON AND IDENTIFICATION OF CAPRINE MYCOPLASMAS ASSOCIATED WITH PNEUMONIA
    (Birsa Agricultural University, 1987) Singh, LONGJAM CHAOBA; Singh, M.P..
    The study of cross activity among caprinenycoplasmasvia.,Mycoplagnapycoides subsp. mycoides. agalactiag and avinini are very important for diagnostic as well as determination of specific, suitable and quick tests for identification purposes. Therefore, studies on the following serological tests were carried out for identification of mycoplagma. 1. In tube agglutination test, antisora raised against three species of caprina mycoplasmas gave a titre of 1 : 320 with their homologous antigens, while, in heterologous system anti-mycoplasma mycoides subsp. mycoidossorum agglutinated agalaction and i arginini antigens upto 1:60 dilution. similarly, anti-agalactiaesorum agglutinated mycoides subsp. mycoidosantiganupto 1:50 dilution while - arvinini only upto 1:40. Anti-agininisarum gave a titre of 1:80 with mycoidas subsp. mycoidas antigen, whereas M. agalactias gave titra only upto 1:40. Thase observations suggest more antigenic structural similarity between - nycoldes subsp. mycolder and Manalactics as compared to - arvinini 2. The agar gel precipitation test revealed at least six precipitation lines among them, two were prominent in homologous system of mycoides subsp. mycoides, Hagalactics and H. arginini. In hotarologous systems only three precipitation lines were observed among the different species of mycoplasmas.