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Subject: Agricultural Biotechnology ×

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    ThesisItemOpen Access
    Analysis for presence of plasmids in antimicrobial producing endophytes of finger millet (Eleusine coracana L. Gaertn.)
    (Birsa Agricultural University, Ranchi, 2019) Kumari, Kushboo; Haider, Z.A.
    The present study has revealed that several endophytes with unique capabilities are part of the finger millet biome. Further study of these endophytes may result in discovery of their potential in agriculture, by way of their ability to combat abiotic and biotic stresses as well as human health by harnessing of substances which could be used as bactericidals.
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    ThesisItemOpen Access
    Molecular analysis of antagonistic effect of Trichoderma to Fusarium in Tomato
    (Birsa Agricultural University, Ranchi, 2019) Prabha, Pragya; Banerjee, Madhuparna
    Tomato (Solanum lycopersicum L.) is a widely grown vegetable crop acquiring the status of world’s most popular vegetable due to its importance for nutritive value, processing and export potential. Tomato is affected by many diseases among which wilt caused by Fusarium oxysporum is one of the important disease, resulting in 10-50 per cent yield loss around the world. Fusarium oxysporum is a serious soil-borne pathogen and persists for longer periods in the soil. Plant treated with Trichoderma viride and Fusarium oxysporum at different time interval. The result revealed that plant treated with Trichoderma viride have altered metabolic activities as they undergo a stress condition. As per our analysis by visual observation the 5 day Trichoderma treated plant with recovery are found more healthy which shows the antagonistic effect of Trichoderma viride against Fusarium oxysporum. This could be due to the beneficial effect of TV in promoting plant growth. Molecular estimation was also performed. DNA extracted from Tomato, Trichoderma viride and Fusarium oxysporum amplified with OPA series primers. And result revealed that primer OPA 13 can distinguish between Tomato, Trichoderma viride and Fusarium oxysporum. CONTENTS PARTICULARS
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    ThesisItemOpen Access
    GENETIC STUDIES IN F2 POPULATION OF RICE (Oryza sativa L.)CROSSES
    (Birsa Agricultural University, Ranchi, 2021) PERWEEN, SHAHINA; Prakash, Surya
    The present investigation in rice (Oryza sativa L.) was undertaken during kharif 2018, at rice research field, Birsa Agricultural University, Kanke, Ranchi, Jharkhand, to study the genetic variability, frequency distribution and correlation analysis for seventeen different characters in F2 population of respective cross combination namely Dhanrashi x Wita-12, Dhanrashi x Shyamala and Sahbhagidhan x Birsamati in rice. The population size was however varied in different cross. The mean and variance of seventeen quantitative characters in F2 population of each crosses indicated a wide range of variability for the characters panicles per plant, grains per plant, spikelets per plant, grains per panicle whereas low variability for 100 grain weight, grain breadth and panicle length. Genotypic coefficient of variation and phenotypic coefficient of variation values were relatively higher for traits like panicles per plant, grain yield per plant, total grains per plant, spikelets per plant and unfilled grains per plant and low to moderate for days to flowering, plant height, primary branches per panicle, panicle length, grain length and grain breadth,100 grain weight in each crosses. In present study the nature of distribution was found platykurtic and positively skewed for traits plant height,total yield per plant, unfilled grains per plant, grain length and L/B ratio in Dhanrashi x Wita- 12 whereas it was observed for the traits days to flowering, primary branches per panicle and secondary branches per panicle in Dhanrashi x Shyamala but in Sahbhagidhan x Birsamati, the traits spikelets per panicle, 100 grain weight, total yield per plant, grain yield per plant, grains per plant, spikelets per plant and L/B ratio was platykurtic and positively skewed. Almost all the characters other than days to flowering were positively and significantly correlated with grain yield per plant and grains per panicle in all three crosses. In the present investigation superior promising individual plants were selected in F2 segregating generation for crop improvement programme.