Loading...
Thesis
Browse
Search Results
ThesisItem Open Access In Vitro Maturation and In Vitro Fertilization of Caprine oocytes and its transfer in Black Bengal Goats(Birsa Agricultural University, Ranchi, 1997) Debashis Chakraborty; J.N.PandeyA large number of embryos can be produced by IVM-IVF technique using abattoir ovaries for research and commercial purpose at a very low cost. The technique involves collection, maturation, fertilization and early embryonic development in suitable media. Oocytes were retrieved from the follicles of goat ovaries collected from slaughter house. The maximum retrieval of cocytes (72.70%) was obtained by dissection technique followed by 61.89% through aspiration and 53.31% through puncture technique. Highest retrieval (38.53%) of good quality pocytes was found through puncture technique followed by dissection (32.42%) and aspiration (25.74%) technique. The fair quality of oocytes were retrieved maximum through aspiration technique (52.23%) followed by dissection (46.93%) and puncture (41.40%) technique. The number of retrieved oocytes/ovary were 5.41 +0.33, 4.54 + 0.14 and 4.09 + 0.80 by dissection, aspiration and puncture technique respectively. Oocytes were cultured in TCM-199 + 10% EDS (medium 1) and TCM - 199 + 10% EDS + 10 IU/ml each of PMSG and HCG (medium 2). Maturation upto metaphase - II indicated a significantly higher proportion of oocytes 65.56% reaching metaphase II in medium 2 compared to 53.79% at the same stage in medium 1. Oocytes from larger follicles have better chance of embryonic development than their smaller counterpart. The incubation timeThesisItem Open Access Reproductive Performance in Prepubertal Gilts following Pregnant mare serum Gonadotropin and Human Chorionic Gonadotropin treatment(Birsa Agricultural University, Ranchi, 1998) Gloria Tigga; S.K.SinhaThe number of male and female piglets in of prepubertal and pubertal group was 48.15: 51.85 and 38.89 61.11 per cent respectively. The Chi-square revealed a non significant difference between these two groups. in value (0.38) the sex ratio litters It may thus be concluded that early maturity reproductive performance (oestrus with behaviour, can acceptable conception rate, gestation length, farrowing percentage) be achieved in prepubertal gilts following exogenous gonadotropins (PMSG and HCG single dose) treatment with good number of offspring at an early age.ThesisItem Open Access Superovulation and Embrya Collection from Prepubertal Goats and its transfer in Adult Block Bengal Goats(Birsa Agricultural University, Ranchi, 1997) Nighat wafai; S.K. SinhaThirty Black Bongal Goats (12 propubortal about 710 months old and 18 adult 112 to 3 years old) were taken for the present study. Out of 12 prepubertal goats, six were subjected to a super ovulatory treatment comprising of PMSG and HCG (Gr.l) and remaining six received no treatment and served as controls (Gr.1l) Eighteen adult goats were also divided into Group-III, Group-IV and Group-V with each having six animals. The goats under Group III also received a super ovulatory treatment (PMSG and HCG) for comparative studies whereas animals under Group IV and Group V served as recipients for Group I and Group III synchronization. Studies receiving PGF, alpha alone for estrus on synchronization, superovulation, embryo collection and its evaluation were carried out in prepubertal and adult goats. Normal transferable embryos were transferred in the adult recipient goats with a synchronous stage of estrus comparable to donor goats. The percentage of success of embryo transfer was evaluated on the basis of kids born. The observations thus recorded are summarized below. The average time interval between PMSG administration and onset of estrus in prepubertal and adult goats was 24.70+3.60 and 27.00+1.29 hours respectively. The variation in the time interval was, however, non-significant statistically. The control group didn't show signs of estrus during the comparative studies.ThesisItem Open Access Lactational Performance and Histo Physiological Changes of Udder Following Induction of Lactation in Goats(Birsa Agricultural University, Ranchi, 1987) Om Prakash MIshra; J.N.PandeyIt is concluded from this study that hormonal induction of lactation using natural and synthetic sex steroids can bring about normal growth and development of the mammary gland in goats. The animals under induced lactation yield milk which is normal in composition and there are no untoward effects on the health of the animals as a result of hormonal treatment.ThesisItem Open Access Locational Performance and his to physiological changes of udder following induction of lactation in goats(Birsa Agricultural University, Ranchi, 1987) Om Prakesh Mishra; J. N. PandeyIt is concluded from this study that hormonal induction of lactation using natural and synthetic sex steroids can bring about normal growth and development of the mammary gland in goats. The animals under induced lactation yield milk which is normal in composition and there are no untoward effects on the health of the animals as a result of hormonal treatment.ThesisItem Open Access Reproduction Performance in Goatd Following Estrus Synchronization And Superovulation(Birsa Agricultural University, Ranchi, 1989) Jshwar, Ajay Kumar; J. N. PandayBased on the above studies it is concluded that estrus synchronization can be successfully achieved through daily injections of progesterone for 16 days or two injec tions of prostaglandin P₂ 11 days apart, without loss of fertility. Supercovulatory dose of 750 IU, PMSG and 500 IU HCG was found to be effectives in increasing the number of mature and ovulated follicles for embryo transfer and other related studies. Changes in the blood metabolites did not reveal any significant deviations from the normal when the animals were treated with progesterone/prostaglandin and subjected to supercovulatory dose of PMSG and HCG, The ovarian histophysiological changes did not indicate any abnormality although the number and sizeof the follicies increased.ThesisItem Open Access STUDIES ON SUPEROVULATION EMBRYO COLLECTION AND ITS EVALUATION FOLLOWING HORMONAL TREATMENT IN BLACK BANGAL GOATS(Birsa Agricultural University, 1988) PANDEY, APARNA; SINHA, S.K.Pregnant more serum gonadotropin administration on day 10 and Prostaglandin administration on day 11 of estrus followed by Husan chorionic gonadotropin administration on the day of estrus were essentially required to synchronize estrus to achieve Maximus number of follicular growth and ovulation and maximum recovery of embryos. Treatment with Prostaglandin on 11th day of the cycle to normal cycling goats caused 100 percent synchronization of estrus. Treatment with HISG was essential for follicular growth and the additional treatment with HCG facilitated ovulation of the mature follicles. The above treatments had no adverse effect on the morphological characters and development of the embryos. It can be safely concluded that collection of embryos on day 4 of laparotomy following the above treatments resulted in better recovery of morphologically normal embryosThesisItem Open Access Studies on certain physiologic performances and milk utilization following induction of lactation in infertile cows(Birsa Agricultural University, Ranchi, 1985) Sinha, Santosh Kumar; Pracad, A.Seven days gonadal steroids treatment were essentially required to develop the mammary glands and to initiate lactation in infertile animals. Success of induction of lactation in 72.21 percent of the infertile cows and heifers under the present study provides a new hope to bring back a majority of such cows into production. Return of certain percentage of treated animals to regular estrus and also to conception and normal calving further widens the scope to transform infertile animals into reproductive status. Additional treatment with chlorpromazine hydrochloride to lactating animals indicated that a significant increased lactation length with increase in total milk production could be achieved whereas, this additional treatment had no gain over the conception rate. The chlorpromazine hydrochloride alone proved to be ineffective to cause growth of the udder and induction of lactation in infertile animals. The composition of milk from induced animals though differed initially from those of freshly parturient cows in respect of protein and fat content but became normal by day 28th of induction of lactation. Biological assay of induced milk carried out on mice and pups revealed the presence of some estrogenic materials in it upto day 30th or prior to it. Further biological assay of the induced milk indicated that though the estrogenic materials or their metabolites remained present in the induced milk, the histophysiological studies suggested that the estrogenic metabolites in such concentrations in the milk of these days had no adverse effects on important regulatory endocrine glands and liver either in mice or pups of both sexes, Lastly it can be safely and strongly advocated that a good percentage of infertile animals could be reconverted into productive and reproductive status, and their milk may be safely consumed after three to four weeks of induction of lactation.ThesisItem Open Access STUDY ON THE EFFECT OF DIFFERENT DOSES OF PROGESTERONE ON POSTPARTUM ANOESTRUS AND FERTILITY IN CROSSBRED CATTLE(Birsa Agricultural University, Kanke, Ranchi, Jharkhand, 2008) KUMAR, MRITUNJAY; Ishwar, A. K.An experiment was conducted on 30 non-lactating anoestrus crossbred cows of age between 5 to 8 years. They were divided in to 5 groups containing 6 animals in each. Different doses of progesterone were injected for different days, intramuscularly. Before start of the experiment animals were dewormed with broadspectrum anthelmintic, (Fenbendazole at the dose rate of 3gm per animal). All the cows detected in estrus were allowed for artificial insemination by frozen semen. Pregnancy was confirmed through per rectum examination after 60 days of insemination. Blood samples (20 ml) from each cow of all groups were collected during fully conscious state by venipuncture of Jugular vein for the studies of different biochemical parameters. Blood samples were collected at day 0 (Just before administration of progesterone), at the end of the drug administration, at the day of estrus and 25th day after the artificial insemination. Group I was the control group as animals were given normal saline but animals of group II were given 50 mg progesterone daily for 15 days. In group III and IV, 100 mg and 150 mg progesterone was administered daily for 10 days. In group III only two (33.33%), in group IV four (66.67) cows exhibited estrus. In group V 250 mg progesterone was administered for 7 days, all the six cows came in to estrus. After artificial insemination one cow in group III, three cows in group IV and all the cows in group V were conceived. At the end of pregnancy one calf from group III two calves from group IV and only four calves from group V were obtained as one cow from group IV and two Cows from group V died due to severe pneumonia before 74 parturition, but were pregnant. Progesterone administration increased the total serum protein significantly in group V that received 250 mg daily for 7 days, compared to control and other groups. Cholesterol is precursor of different steroidal hormone. Its level can indicate circulating adequacy of these hormones responsible for normal oestrus. Cholesterol level was significantly (P<0.01) highest in case at oestrus period in group V compared to control and other groups. There is certain level of inorganic Phosphorus for estrogen secretion. In our study, there was no supplementation of P in diet even there was significant (P<0.01) increase of serum Phosphorus level in group V compared to control and other groups. There was also significant (P<0.01) increase of SGPT (ALT) and ALP level in blood serum of crossbred cows showing the high physiological activity of enzymes in case of progesterone administration in group V compared to control and other groups. No animal was in milking stage, so the effect of exogenous progesterone on milk production in cattle was not observed. In conclusion, most preferred dose of progesterone for the induction of oestrus cyclicity and fertility in anoestrus crossbred cows was 250 mg daily for seven days. Although we did not achieve the proper synchronizing time, so, it is suggested that in group V instead of seven days we can enhance one or two days more treatment with the same dose, although the conception and fertility rate was up to the desired level.
