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Subject: Microbiology × Author: AHLUWALIA, NEHA ×
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    ThesisItemOpen Access
    EFFECT ON GROWTH OF PEA SEEDLINGS BY PHOSPHATE SOLUBILIZING RHIZOBIUM LEGUMINOSARUM
    (DR. YASHWNAT SINGH PARMAR UNIVERSITY OF HORTICULTURE AND FORESTRY NAUNI SOLAN, 2009) AHLUWALIA, NEHA; SHIRKOT, C. K.
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    ThesisItemOpen Access
    EFFECT ON GROWTH OF PEA SEEDLINGS BY PHOSPHATE SOLUBILIZING Rhizobium leguminosarum
    (2009) AHLUWALIA, NEHA; SHIRKOT, C.K.
    ABSTRACT Rhizobium leguminosarum isolated originally isolated from root nodules of pea was tested for its ability to solubilize tricalcium phosphate (TCP). This strain showed solubilization on Pikovaskaya’s agar medium with 1.38 solubilization index and solubilized a substancial amount of TCP 785 mg/ml in PVK broth. Among the carbon sources, mannitol was found to be best carbon source with yeast extract as bets nitrogen source showing maximum P-solubilization 787.00 mg/l and 797.30 mg/l and % growth inhibition 41.10 and 40.12 percent against Fusarium oxysporum. While 28 ºC temperature, 7.5 pH, 72 h incubation period and 0.5 % TCP concentration were found to be best for the growth and P-solubilization by Rhizobium leguminosarum in yeast extract mannitiol broth. Concurrent production of IAA, siderophore, antifungal activity along with phosphorus solubilization revealed its plant growth promoting potential. Seed treatment with charcoal based culture of Rhizobium leguminosarum with TCP amendment increased the germination percent of pea seeds under growth chamber as well as in net house conditions. Among different TCP concentration 262 mg/ 2 Kg of soil showed maximum shoot length, root length, shoot dry weight and root dry weight. Thus the ability of per forming multifarious activities in tandem suggesting Rhizobium leguminosarum a novel strain for plant growth promotion and has significance to develop cost effective ecofriendly sustainable system in the management of environmental and agriculture problems.