EFFECT ON GROWTH OF PEA SEEDLINGS BY PHOSPHATE SOLUBILIZING Rhizobium leguminosarum
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Date
2009
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Abstract
ABSTRACT
Rhizobium leguminosarum isolated originally isolated from root nodules of pea was tested for its
ability to solubilize tricalcium phosphate (TCP). This strain showed solubilization on
Pikovaskaya’s agar medium with 1.38 solubilization index and solubilized a substancial amount
of TCP 785 mg/ml in PVK broth. Among the carbon sources, mannitol was found to be best
carbon source with yeast extract as bets nitrogen source showing maximum P-solubilization
787.00 mg/l and 797.30 mg/l and % growth inhibition 41.10 and 40.12 percent against Fusarium
oxysporum. While 28 ºC temperature, 7.5 pH, 72 h incubation period and 0.5 % TCP
concentration were found to be best for the growth and P-solubilization by Rhizobium
leguminosarum in yeast extract mannitiol broth. Concurrent production of IAA, siderophore,
antifungal activity along with phosphorus solubilization revealed its plant growth promoting
potential. Seed treatment with charcoal based culture of Rhizobium leguminosarum with TCP
amendment increased the germination percent of pea seeds under growth chamber as well as in
net house conditions. Among different TCP concentration 262 mg/ 2 Kg of soil showed
maximum shoot length, root length, shoot dry weight and root dry weight. Thus the ability of per
forming multifarious activities in tandem suggesting Rhizobium leguminosarum a novel strain for
plant growth promotion and has significance to develop cost effective ecofriendly sustainable
system in the management of environmental and agriculture problems.