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2006 - 200922010 - 20191
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Subject: Biotechnology × Author: KUMAR, VINAY ×
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    ThesisItemOpen Access
    STUDIES ON IN VITRO REGENERATION OF ENDANGERED MEDICINAL PLANT NAG CHHATRI (TRILLIUM GOVANIANUM WALL. EX D. DON)
    (UHF,NAUNI, 2019-12) KUMAR, VINAY; SHARMA, RAJNISH
    ABSTRACT The present investigations were aimed at “Studies on in vitro regeneration of endangered medicinal plant nag chhatri (Trillium govanianum Wall. ex D. Don)”. Plant material was procured from the natural habitat and different parts viz., seeds, roots, rhizome discs, rhizome buds, stems and leaves were used as explants for in vitro regeneration. Highest per cent survival 86.67%, 84.45%, 83.33% and 93.33% was observed in seed, root, rhizome disc and rhizome bud explants, respectively when treated with 0.2% carbendazim for 5 mins along with 0.1% HgCl2 for 3 mins while phytotoxic effect of different sterilants was observed on leaf and stem explants. Out of different explants used, rhizome buds showed 96.67% shoot regeneration on MS medium supplemented with 0.5 mg/l BAP, 0.5 mg/l kinetin, 0.5 mg/l GA3 with average shoot length of 4.33 cm after 4 weeks of culturing. Modified MS medium containing ammonium sulphate, 7.00 mM CaCl2and 0.2 mM Fe-EDDHA was used for shoot regeneration to overcome the problem of leaf chlorosis that was observed after one week from shoot proliferation. Substantial increase in the production of lateral buds and mini rhizomes was found on MS medium enriched with 6% sucrose, 0.5 mg/l BAP, 0.5 mg/l kinetin, 0.5 mg/l GA3 and 100 mg/l casein hydrolysate with 93.33% bud production. Higher concentration of sucrose, growth regulators (BAP & TDZ) and additives (casein hydrolysate & L-glutamine) showed significant effect on mini rhizomes production from in vitro derived lateral buds. Number of experiments were carried out for inducing in vitro rooting in regenerated shoots and mini rhizomes using different auxins on half strength and full strength media, respectively, but rooting couldn’t be achieved because of the browning of established shoots and mini rhizomes. Therefore, it was inferred that the present study would be helpful towards various in vitro conservation practices of this valuable medicinal herb in near future. Keywords: In vitro regeneration,Trillium govanianum, Rhizome, Lateral bud, Mini rhizome, Medicinal herb
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    ThesisItemOpen Access
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    ThesisItemOpen Access
    PARTIAL MOLECULAR CHARACTERIZATION OF WILD AND CULTIVATED POMEGRANATE Punica granatum L.
    (UHF,NAUNI, 2006) KUMAR, VINAY; Kanwar, Kamlesh
    The present investigation on "Partial molecular characterization of wild and cultivated pomegranate (Punica granatum l.)" allows us to determine the phenotypic as well as genetic variability among pomegranate cultivars. Six pomegranate cultivars Daru (Wild pomegranate). Ganesh, G·137, Mridula, Musket and Kandharl Kabuli were taken for characterization and evaluation. Twelve morphological parameters were used for evaluation. Kandhari Kabuli was the best cultivars among six regarding fruit weight (g}, seeds/fruit, seeds weight (9) and more so seed taste. Four enzymes systems (PER, ACP, ALP and EST) were used for characterization. In RAPD study, 21 primers were used and yielded 198 scorable bands out of which 151 were polymorphic. Thirty unique bands were observed among 6 cultivars during the investigati\: The Jaccard's coefficient and UPGMA based analysis showed 0.62 to 0.83 similarities among these cultivars, also in cluster analysis Daru (Wild pomegranate) found to be far away from other cultivars. Lowest value of 0.62 was found between Ganesh and G-137, whereas maximum value of 0.83 was found between Kandhari Kabuli and Musket. This revealed that the origin of all cultivar from same parent but long ago.