Thumbnail Image

Theses

Browse

2008 - 200922010 - 20111
Reset filters
Subject: Biotechnology × Author: KASHYAP, MONIKA ×
Reset filters

Search Results

Now showing 1 - 3 of 3
  • Thumbnail Image
    ThesisItemOpen Access
    STUDIES ON GENE TAGGING FOR RESISTANCE TO XANTHOMONAS CAMPESTRIS IN CAULIFLOWER USING SSR MARKERS
    (COLLEGE OF HORTICULTURE DR. YASHWANT SINGH PARMAR UNIVERSITY OF HORTICULTURE AND FORESTRY, 2008) KASHYAP, MONIKA; KAUR, RAJINDER
  • Thumbnail Image
    ThesisItemOpen Access
    STUDIES ON GENE TAGGING FOR RESISTANCE TO Xanthomonas campestris IN CAULIFLOWER (Brassica oleracea var. botrytis) USING SSR MARKERS
    (UHF,NAUNI, 2008) KASHYAP, MONIKA; KAUR, RAJINDER
    The present investigation on cauliflower (Brassica oleracea var. bouytis) was carried out to tag gene for resistance to Xanthomonas campestris using SSR markers. F2 population, raised from the cross between Winter Cauliflower (Armel) (resistant parent) and Pusa Snowball (susceptible parent), was used as the mapping population. DNA was extracted from both the parental lines as well as 154 F2 progenies by using CTAB method, purified and q~antified at 50 ng/μl. F2 plants were screened for resistance to Xanthomonas campestris by inoculating with bacterial suspension, and out of 154, 113 resistant and 41 susceptible plants were found. The goodness of fit of observed ratio (3: 1) was tested by Chi-square test with r.!= 0.261, hence confinning that the .resistance is controlled by single dominant gene. A totaJ of 35 SSR primer pairs were screened, out of which 10 primers were found to be monomorphic and 25 primers were polymorphic. However, only six primers produced polymorphic banding pattern between both the parents and bulks and used for bulked segregant analysis (BSA). Primer PBCGSSRBolO amplified .a single SSR band of 800 bp that only appeared in resistant parent, resistant bulk and ten resistant f 2 segregants, hence indicating its Linkage with resistance gene. The marker was found to be at a distance of2.6 cM from the target locu.s.
  • Thumbnail Image
    ThesisItemOpen Access
    Effect of antibiotics on shoot regeneration and suppression of Agrobacterium growth in apple rootstock
    (NAUNI,SOLAN,UHF, 2011) KASHYAP, MONIKA; MODGIL, MANJU
    ABSTRACT The present investigation aims at “Studies on cell culture for genetic transformation in Punica granatum L.”. Genetic transformation experiment was conducted with cotyledonary explants excised from in vitro raised seedlings of Punica granatum L. cv. Kandhari Kabuli. Explants were preconditioned on standardized callus induction medium (solid MS medium supplemented with 4.0 mg/l BA and 3.0 mg/l NAA) for 48 hours followed by 48 hours of cocultivation with Agrobacterium tumefaciens. Friable putative transgenic were used for initiation of cell suspension culture. Cells of various shapes such as oval, elongated, columnar, elliptical and isodiametric were observed in the suspension culture. Cells proliferated best on selective MS medium supplemented with 4.0 mg/l BA + 3.0 mg/l NAA and 50 mg/l kanamycin along with 500 mg/l cefotaxime with average cell doubling time of 5 days. Combination of a cytokinin (BA) and an auxin (NAA) was found to be better for cell proliferation as compared with the combination of two cytokinins (BA and TDZ). Growth curve of cells in suspension culture consisted of lag phase (5-6 days), exponential phase (7 days) and stationary phase (4-5 days) followed by cell death. Plating efficiency of 0.7 % was obtained by plating of cell aggregates (5-6 cells) on selective MS medium supplemented with 4.0 mg/l BA and 3.0 mg/l NAA utilizing modified Bergman technique. Putative transgenic macrocalli did not show shoot regeneration. Positive PCR results were observed in transgenic calli obtained through cell suspension culture. The protocol, thus offer an opportunity to produce the putative transformed tissues/ plantlets with single cell origin.