STUDIES ON GENE TAGGING FOR RESISTANCE TO Xanthomonas campestris IN CAULIFLOWER (Brassica oleracea var. botrytis) USING SSR MARKERS
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Date
2008
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UHF,NAUNI
Abstract
The present investigation on cauliflower (Brassica oleracea var. bouytis)
was carried out to tag gene for resistance to Xanthomonas campestris using SSR
markers. F2 population, raised from the cross between Winter Cauliflower
(Armel) (resistant parent) and Pusa Snowball (susceptible parent), was used as
the mapping population. DNA was extracted from both the parental lines as well
as 154 F2 progenies by using CTAB method, purified and q~antified at 50 ng/μl.
F2 plants were screened for resistance to Xanthomonas campestris by inoculating
with bacterial suspension, and out of 154, 113 resistant and 41 susceptible plants
were found. The goodness of fit of observed ratio (3: 1) was tested by Chi-square
test with r.!= 0.261, hence confinning that the .resistance is controlled by single
dominant gene. A totaJ of 35 SSR primer pairs were screened, out of which 10
primers were found to be monomorphic and 25 primers were polymorphic.
However, only six primers produced polymorphic banding pattern between both
the parents and bulks and used for bulked segregant analysis (BSA). Primer
PBCGSSRBolO amplified .a single SSR band of 800 bp that only appeared in
resistant parent, resistant bulk and ten resistant f 2 segregants, hence indicating its
Linkage with resistance gene. The marker was found to be at a distance of2.6 cM
from the target locu.s.
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