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Subject: Biotechnology × Author: KASHYAP, MONIKA × End date: 2009 × Start date: 2000 × Type: Thesis ×
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    ThesisItemOpen Access
    STUDIES ON GENE TAGGING FOR RESISTANCE TO XANTHOMONAS CAMPESTRIS IN CAULIFLOWER USING SSR MARKERS
    (COLLEGE OF HORTICULTURE DR. YASHWANT SINGH PARMAR UNIVERSITY OF HORTICULTURE AND FORESTRY, 2008) KASHYAP, MONIKA; KAUR, RAJINDER
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    ThesisItemOpen Access
    STUDIES ON GENE TAGGING FOR RESISTANCE TO Xanthomonas campestris IN CAULIFLOWER (Brassica oleracea var. botrytis) USING SSR MARKERS
    (UHF,NAUNI, 2008) KASHYAP, MONIKA; KAUR, RAJINDER
    The present investigation on cauliflower (Brassica oleracea var. bouytis) was carried out to tag gene for resistance to Xanthomonas campestris using SSR markers. F2 population, raised from the cross between Winter Cauliflower (Armel) (resistant parent) and Pusa Snowball (susceptible parent), was used as the mapping population. DNA was extracted from both the parental lines as well as 154 F2 progenies by using CTAB method, purified and q~antified at 50 ng/μl. F2 plants were screened for resistance to Xanthomonas campestris by inoculating with bacterial suspension, and out of 154, 113 resistant and 41 susceptible plants were found. The goodness of fit of observed ratio (3: 1) was tested by Chi-square test with r.!= 0.261, hence confinning that the .resistance is controlled by single dominant gene. A totaJ of 35 SSR primer pairs were screened, out of which 10 primers were found to be monomorphic and 25 primers were polymorphic. However, only six primers produced polymorphic banding pattern between both the parents and bulks and used for bulked segregant analysis (BSA). Primer PBCGSSRBolO amplified .a single SSR band of 800 bp that only appeared in resistant parent, resistant bulk and ten resistant f 2 segregants, hence indicating its Linkage with resistance gene. The marker was found to be at a distance of2.6 cM from the target locu.s.