Loading...
Thesis
Browse
11 results
Search Results
ThesisItem Open Access COMPARATIVE EVALUATION OF WOUND HEALING PROPERTIES OF NANOSILVER AND BOSWELLIA OVALIFOLIOLATA MEDIATED NANOSILVER IN WISTAR RATS(Sri Venkateswara Veterinary University, TIRUPATI – 517 502,A.P, 2012-10) APARNA REDDY, N; ESWARA PRASAD, P (Major); PADMAJA, K; ADILAXMAMMA, KABSTRACT : An experimental study was conducted to evaluate and compare the wound healing properties of citrate coated silver nanoparticles and B.ovalifoliolata mediated silver nanoparticles in wistar rats in three wound models. The nanoparticles were synthesized and the synthesized nanoparticles were characterized using UV-visible spectrophotometer, Scanning electron microscope (SEM), DLS (Dynamic light scaterring) Technique and FT-IR analysis and were also evaluated for antimicrobial activity and cytotoxic effects. A total of 90 rats (both sexes) were divided into five groups, each group containing 18 rats and the wound healing activity was evaluated in three different wound models viz., excision, incision and dead space model. Group 1 was treated with normal saline, group 2 with Povidone iodine (1% solution), group 3 with Citrate coated silver nanoparticles (AgNPs) (170 μg per dressing), group 4 received B.ovalifoliolata bark ethanolic extract (50 mg per dressing) and group 5 received B.ovalifoliolata mediated silver nanoparticles (BENS) (85 μg per dressing). The respective concentrations of the drug were prepared as dressings and applied topically. In excision wound model, percentage wound contraction and the periods of epithelialization were recorded. Blood was collected from orbital sinus in EDTA coated tubes and plasma was separated for estimation of Vitamin C, TBARS and protein. Granulation tissue from excision model was processed for histopathological examination. In incision wound model, the wound breaking strength was measured on 10th day. In dead space wound model, the granulation tissue collected on Day 10 was used for the estimation of hydroxyproline, hexosamine, total protein, TBARS, superoxide dismutase, catalase, reduced glutathione and ascorbic acid. UV Visible absorption spectrum showed a maximum absorption between 350-450 nm and around 450 nm for AgNPs and BENS respectively. SEM images showed that AgNPs showed an agglomerated morphology (size 374-718 nm) while, B.ovalifoliolata mediated silver nanoparticles were spherical in shape (size 192.8-346.1 nm). The results of FT-IR analysis indicated the involvement of hydroxyl, carboxyl and amine groups in the synthesis of B.ovalifoliolata mediated silver nanoparticles. The size of the particles as measured by DLS technique was 78.8 and 273.5 nm respectively. The in vitro studies showed that BENS possess highest antibacterial activity against Staphylococcus aureus as evidenced by highest zone of inhibition (17.67 mm) and lowest MIC value (1.77μg/ml). BENS was practically nontoxic at 85 ppm while AgNPs were toxic from 10 ppm onwards. In excision wound model, BENS showed significantly (p<0.05) higher percentage of wound contraction and shortest period of epithelialization compared to control group indicating better wound healing property. The TBARS level was significantly (p<0.05) lower and the levels of vitamin C and protein were elevated in both BE and BENS groups. Histopathological examination of BENS group showed mild cellular infiltration with angiogenesis and more fibroblast proliferation when compared to control. In the incision wound model, BE and BENS groups showed significantly (p<0.05) higher wound breaking strength compared to other groups. In dead space wound model, there was a significant increase in hydroxyproline and hexosamine content with increased catalase and SOD activity and decreased lipid peroxide levels in the granulation tissue of BENS group compared to other treatment groups. Thus in conclusion B.ovalifoliolata mediated silver nanoparticles showed better wound healing properties compared to citrate coated silver nanoparticles and ethanolic extract of B.ovalifoliolata.ThesisItem Open Access EFFECT OF Caesalpinia sappan EXTRACTS ON PRESERVATION OF MEAT(Sri Venkateswara Veterinary University, TIRUPATI – 517 502,A.P, 2011-12) VENKATASAIKUMAR, T; ESWARA PRASAD, P (Major); PADMAJA, K; JAGADEESH BABU, AABSTRACT : An experiment was conducted to study the effect of C.sappan extracts on preservation of meat. This study was carried out to evaluate the antimicrobial and antioxidant activities of C.sappan extracts ( aqueous, methanolic), methanolic extract of C.sappan along with antibiotic (chlortetracycline) as natural food preservative in chicken, pork and mutton The results were compared with conventional methods like acetic acid and chlorinated water treatment on meat preservation. The experiment was carried out in two phases. In phase I, aqueous and methanolic extracts of C.sappan, methanolic extract of C.sappan along with antibiotic (chlortetracycline), acetic acid and chlorinated water at different dilutions were prepared and were evaluated to find MIC using agar diffusion assay. The results obtained in the phase I revealed that the aqueous extract of C. sappan did not exhibit any inhibitory effect on pathogenic organisms tested and hence it was not used for further study. However methanolic extract C.sappan, methanolic extract of C.sappan along with antibiotic (chlortetracycline), acetic acid and chlorinated water have exhibited high inhibitory action on pathogenic bacteria at dilutions of 1:5, 1:5, 4% and 300ppm respectively. In second phase, a control and four treatment groups namely - methanolic extract of C. sappan plus antibiotic (T1), methanolic extract of C. sappan (T2), acetic acid (T3) and chlorinated water (T4) were formed to study their effect on preservation of different species of meat. Raw chicken, pork and mutton samples were obtained from local retail meat market, each sample was treated with methanolic extract of C. sappan plus antibiotic (T1), methanolic extract of C. sappan (T2), acetic acid (T3) and chlorinated water (T4) and effect of the treatments were evaluated using different parameters. There was no significant difference found between T1 and T2 for the parameters such as pH, TBARS, free fatty acid, peroxide, yeast and mould count, colour and flavour. For the parameters such as total platecount psychrophiles, coliforms, MRSA, Salmonella counts all treatments differed significantly(P<0.05). The effect in reduction of microbial load in meat samples was highest in T1 followed by T2, T3 and T4. The mean values of parameters (pH, TBARS, free fatty acid, peroxide, yeast and mould count, total platecount, psychrophiles, coliforms, MRSA, Salmonella counts) increased significantly from 0 to 8th day irrespective of type of treatment. The values for color and flavour decreased significantly as the period of storage increased. The results of the experiment concludes that methanolic extract of C. sappan and methanolic extract of C. sappan with antibiotic significantly exhibited beneficial effects on meat preservation as compared to other conventional treatments and these effects were almost similar on different species of meat samples. The results also indicated that pH, TBARS, free fatty acid, peroxide value and microbial count increased along with period of storage irrespective of treatment.ThesisItem Open Access MOLECULAR DETECTION AND CONTROL OF SALMONELLA IN ANIMAL FEEDS(Sri Venkateswara Veterinary University, TIRUPATI – 517 502,A.P, 2010-10) SRAVANTHI, MANNEM; ESWARA PRASAD, P (Major); SUDHAKARA REDDY, K; SREENIVASULU, D; ADILAXMAMMA, KABSTRACT : Feed and feed ingredients serve as a carrier for a range of bacteria, which can adversely affect the performance of animal. One such important contaminant of feeds is Salmonella species. Traditional cultural methods used for identification of Salmonella involves use of pre-enrichment, selective enrichment broths (Selenite cystine broth or Rappaport Vassiliadis broth), selective enrichment media (BGA, MCA, XLD etc.) and confirmation of isolates by biochemical tests. Among 109 feed samples tested, seven (6.42%) positive Salmonella isolates were obtained. The incidence of Salmonella contamination in animal feed samples was screened as 10.81% in Kadapa where as 4.4% in Chittoor district. The traditional cultural methods used for Salmonella isolation were time consuming. Among the several alternate analysis strategies, Polymerase Chain Reaction has been found to be a highly specific molecular diagnostic tool. PCR technique was standardized targeting fimA gene of Salmonella. A 29 cycle PCR reaction with an annealing temperature of 58°C and MgCl2 concentration of 3 mM were found optimum for amplification of 85 bp product of fimA gene. To overcome the production losses due to the presence of bacteria in feed samples, suitable control methods have to be followed. Three different control methods viz., formaldehyde treatment, UV treatment and sun drying were adopted. Formaldehyde at two concentrations (0.5% and 1%) and UV light at a wavelength range of 250-260 nm for 10 minutes were used for treatments. Feed samples were kept for sun drying for a period of 6 days. After treatments, feed samples were again processed for Salmonella isolation and total plate counts. All the three treatment methods were effective in completely decreasing the Salmonella levels in feed samples. For reducing total bacterial counts formaldehyde treatment found to be the most effective method, followed by UV treatment and sun drying. ABST was carried out to determine the drugs, effective for the therapy of Salmonella. The drugs, ofloxacin, ciprofloxacin, and ceftriaxone were found to be effective for all isolates of Salmonella. The results of the experiment conclude that PCR technique with fimA gene is found to be effective in detecting Salmonella from feed samples at a shorter time and 1% formaldehyde treatment is highly effective in reducing the total counts of bacteria. Results of ABST conducted indicated ofloxacin, ciprofloxacin and ceftriaxone are highly effective against Salmonella as compared to the other antibiotics.ThesisItem Open Access A STUDY ON AMELIORATING EFFECT OF CAESALPINIA SAPPAN L IN LEAD INDUCED OXIDATIVE STRESS IN RATS(Sri Venkateswara Veterinary University, TIRUPATI – 517 502,A.P, 2010-09) HEMALATHA, K; ESWARA PRASAD, P (Major); PADMAJA, K; ADILAXMAMMA, K; SREENIVASULU, DABSTRACT : An experiment was conducted to study the ameliorating effect of Caesalpinia sappan in lead toxicity using a total of 24 male wistar rats which were randomly divided into four groups consisting of six animals in each group. Group I was maintained on basal diet as control and group II on lead acetate @ 90 mg/kg orally along with basal diet as toxic control group. Group III was maintained on methanolic extract of Caesalpinia sappan stem bark @ 100 mg/kg along with the basal diet and group IV on lead acetate @ 90 mg/ kg and methanolic extract of Caesalpinia sappan @ 100mg/ kg along with the basal diet for 28 days. The serum biomarkers were estimated for assessing the damage caused to liver, kidney and heart due to lead toxicity. Parameters like total protein, ALT, troponins, AST, creatinine and BUN values were measured on Day 14 and Day 28 from freshly collected blood samples. Tissue samples were collected and preserved at -20ᵒC for analysis of tissue antioxidant profile (TBARS, glutathione, GST, SOD, catalase, membrane ATPases, Vitamin E and C) and lead content. Blood samples were collected and haematological parameters (RBC, WBC, Hb and PCV) were estimated at the end of 28th day of experiment. The results indicated that lead toxic group (group II) showed a significant (p<0.05) decrease in serum total proteins and significant (p<0.05) increase in ALT, troponins, AST, creatinine and BUN values revealing that lead has induced damage to the liver, kidney and heart tissues. Significant decrease in total RBC count, haemoglobin and PCV were observed in group II which showed the evidence of damaging effect of haemopoietic system due to lead toxicity. The mean values of TBARS were significantly increased in lead control group where as the levels of glutathione, GST, SOD, total membrane ATPases and Vitamin E were significantly decreased in group II indicating lead induced oxidative damage to the tissues. Significant increase in the levels of lead was observed in liver and kidney in group II. Treatment with methanolic extract of Caesalpinia sappan stem bark (group IV) resulted in improvement in above parameters as compared to lead toxic group (group II). Lead quantity of the liver was reduced slightly in group IV compared to group II but could not bring the levels to normal. Thus, it is concluded that lead induces biological damage to liver, kidney and heart tissues and further caused oxidative stress and Caesalpinia sappan offered amelioration which is attributed to its antioxidant activity but it could not able to reduce the lead content of the tissues completely.ThesisItem Open Access STUDIES ON EXPRESSION OF INSULIN LIKE GROWTH FACTOR BINDING PROTEIN 3 (IGFBP 3) GENE IN CULTURED AND IN VIVO DEVELOPED PREANTRAL FOLLICLES IN SHEEP(Sri Venkateswara Veterinary University, TIRUPATI – 517 502,A.P, 2010-10) SIVA SAGAR REDDY, K; RAO, V.H (Major); SIVA KUMAR, A.V.N; PADMAJA, KABSTRACT: Expression of Growth Differentiation Factor 9 (GDF 9) and Insulin like Growth Factor Binding Protein 3 (IGFBP 3) genes was studied in oocytes and granulosa cells isolated from various in vivo and in vitro follicular development stages in sheep. In the present study, the expression of both the genes was highest in the primordial follicles. The expression of GDF 9 in in vivo and in vitro oocytes decreased as the development progressed except in large antral follicles (OA2). Among the granulosa cells, the expression of GDF 9 in in vivo stages decreased continuously. In the in vitro granulosa cell stages, the expression of GDF 9 increased except PFs’ cultured for 6 days (G6). The expression of IGFBP 3 in in vivo and in vitro oocytes increased as the development progressed except in antral follicles (OA1). Among the granulosa cells, the expression of IGFBP 3 in in vivo and in vitro stages increased as the development progressed except PFs’ cultured for 6 days (G6). When the oocytes from large antral follicles (OA2) and 6 day cultured PFs’ (O6) were matured in vitro for 24h, the expression of both the genes decreased. When the granulosa cells from large antral follicles (GA2) and 6 day cultured PFs’ (G6) were matured in vitro for 24h, the expression of both the genes increased. The expression of both the genes increased significantly in the oocytes and granulosa cells from in vitro cultured PFs’ than their corresponding in vivo stagesThesisItem Open Access MICROSATELLITE MARKER BASED ASSESSMENT OF GENETIC STRUCTURE OF MACHERLA BROWN SHEEP(SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI – 517502. (A.P.) INDIA, 2015-12) MOHAN KISHORE, MOLABANTI; Punya kumari, B (Major); Jaya laxmi, P; Suresh, JABSTRACT: Macherla Brown sheep is the native sheep distributed along the banks of river Krishna flowing through Guntur, Krishna, Prakasam and Nalgonda districts of Andhra Pradesh and Telangana states. The home tract is mainly from the Nagarjuna sagar dam areas of Guntur and Nalgonda. Macherla Brown is known for heat tolerance, disease resistance and thrives well in harsh climatic conditions and scarce feeding conditions. The assessment of genetic variation is important to characterize the populations for breed recognition, conservation and also to determine the level of inbreeding in the population. The present study was carried out to assess the genetic structure of Macherla Brown sheep at molecular level to understand the genetic variability and diversity of population and to recognise this high performing local variety of sheep as a breed. Twenty four ovine microsatellite markers recommended by ISAG/FAO for population diversity studies in sheep were used in the present study. Phenol-chloroform method was used for the isolation of DNA from the collected blood samples and the microsatellite markers were amplified by PCR reaction. The amplicons were identified using agarose gel electrophoresis. The genotypes were scored using regression equation of the 100 bp DNA standard. The number, size and frequency of alleles were calculated using POPGENE version 1.3.2 software. A total of 275 alleles were observed for the twenty four marker loci under investigation in the sampled Macherla Brown population. The number of alleles at each locus varied from a minimum of eight (BM6506, HSC, OarCP34) to a maximum of eighteen (MAF214) with a mean of 11.458 alleles. Allele size ranged from a minimum of 69 bp (OarCP20) to a maximum of 313 bp (MAF214), while allele frequency varied between 0.0102 (HSC, INRA63, MAF214, OarP49 and OarVH72) to 0.2551 (HSC). The number of effective alleles across twenty four microsatellite loci studied ranged from 5.8065 (HSC) to 14.1652 (OarFCB48). The mean number of effective alleles was 9.597 ± 2.454. The observed heterozygosity ranged from 0.0612 (BM8125) to 0.2653 (OarCP34) with a mean value of 0.110 ± 0.049 whereas, the expected heterozygosity ranged from 0.8278 (HSC) to 0.9294 (OarFCB48) with a mean value of 0.889 ± 0.029. All the twenty four microsatellite loci (100 percent) were found to be highly polymorphic and the PIC values ranged from 0.8052 (HSC) to 0.9295 (OarFCB48). The mean PIC value for all the twenty four loci was found to be 0.882 ± 0.036. The Chisquare test revealed that all the twenty four loci were showing significant deviation from Hardy-Weinberg Equilibrium. The inbreeding estimates obtained in this study were all positive and ranged from 0.6915 (OarCP34) to 0.9341 (OarFCB48) with the mean FIS value of 0.876 ± 0.057 indicating the high deficiency of heterozygotes.ThesisItem Open Access REPAIR OF FEMORAL SHAFT FRACTURES BY ELASTIC PLATE OSTEOSYNTHESIS USING VETERINARY CUTTABLE PLATES IN YOUNG DOGS(SRI VENKATESWARA VETERINARY UNIVERSITY , TIRUPATI – 517 502 , A.P, 2015-10) SUDARSHAN REDDY, K; DHANA LAKSHMI, N (Major); VEENA, P; RAJU, N.K.B.ABSTRACT : Young dogs presented to the College Hospital with unstable femoral diaphyseal fractures were diagnosed by clinical signs, orthopaedic examination and survey radiography. The breed, age and sex wise incidence, etiology and type of fracture were studied. Twelve immature young dogs with femoral diaphyseal fractures (2-5 months old, body weight ranging from 4-9 kg) were selected and stabilized with indigenously designed Veterinary Cuttable Plate (VCP) along with compatible size of cortical screws by Elastic Plate Osteosynthesis (EPO) technique. The femur was approached by cranio-lateral border of thigh for plating under general anaesthesia. Selection of plate and screws was determined by taking into consideration of the size of the bone, fracture configuration and weight of the dogs. Indigenously designed VCP (2 mm for dogs below 5 Kg and 2.7 mm for 5-9 kg body weight) used with compatible size of non self tapping cortical screws for stabilization of fractures in each animal provided good fracture stability. The technique increased overall compliance of the construct with the use of plate with 2 or 3 screws at each fracture fragment when applied over thin cortices to reduce the risk of focal failure of the screw/bone interface. No technical difficulties were observed while application of VCP by Elastic Plate Osteosynthesis technique in all cases. The outcome of fracture stabilization and healing was evaluated with postoperative lameness grading, radiography and biochemical analysis. All animals, which were diagnosed with unstable femoral diaphyseal fractures showed grade V lameness before surgical management. Postoperatively, all cases showed grade I lameness in an average of 5-7 weeks. Functional limb outcome was excellent to good. The fractures showed radiographically excellent postoperative healing of cortical union, absence of fracture lines with periosteal and bridging callus in an average 3-5 weeks and further remodelling changes with complete cortical union were observed in all cases by 7 weeks. Postoperative fracture fragment alignment was not affected except in case No.7 where healing was delayed. Statistical analysis, the mean ± SE values revealed a highly significant difference (P<0.01) in serum alkaline phosphatase and calcium. C-reactive protein values in different stages of postoperative significantly increased from 0 day to 14th day and thereafter gradually decreased in levels. No major intraoperative complications were observed. Postoperatively, delayed cortical union was observed when compared to other cases in case No.7 where wound dehiscence with distal screw pullout was observed by 5th day in which revision surgery was performed and but overall outcome was not affected. Slight bending of plate at fracture site in case No.10 was noticed which did not show any malalignment of fractured fragments. Complete removal of implant was done on an average 5-7weeks, after radiographic appearance of cortical union. Based on present study, Elastic Plate Osteosynthesis technique (EPO) using VCP (narrow, thin and cut to desired length) was better suited for the treatment of femoral diaphyseal fractures in young dogs with thin cortical bones and active growth plates. EPO technique was more advantageous than rigid plate osteosynthesis by reducing the stress shield effect on bone and hastened the bone healing by allowing micromotion at the fracture site to closely mimic biological healing as it did not interfere with functional growth plates in young dogs and also to minimize the fracture disease which was observed in intramedullary pinning. Implant used in this technique is not much expensive, making it suitable for use in Veterinary practice.ThesisItem Open Access CLINICO- DIAGNOSTIC AND THERAPEUTIC STUDIES ON BOVINE RESPIRATORY DISEASE ASSOCIATED WITH BACTERIAL INVOLVEMENT(SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI-517502. (A.P) INDIA, 2015-11) SREENIVASA REDDY, P; VAIKUNTA RAO, VABSTRACT : The aim of study is to identify the etiological agents in bovine respiratory disease associated with bacterial involvement, to study the clinical signs, haemato-biochemical and radiographic changes in bovine respiratory disease associated with bacterial involvement and to assess the efficacy of adopted therapeutic regimen for bovine respiratory disease associated with bacterial involvement. The study revealed that occurrence of respiratory disease with bacterial involvement was 23.05 per centage. In the present study, buffaloes were more affected than cattle. Cultural isolation and identification of nasal discharges from the clinical cases revealed P.multocida, Pseudomonas spp, Klebsiella spp and E.Coli. The P.multocida was also identified from the blood samples of the clinical cases by PCR. Twenty five buffaloes were found positive for malignant catarrhal fever with PCR. The most prominent clinical signs observed in bovine respiratory disease with bacterial involvement were respiratory distress, anorexia, nasal discharges, congested conjunctival mucous membrane, muzzle dryness, cough, oral breathing and sneezing. The prominent physical findings were dyspnoea, tachycardia, tachypnoea, pyrexia, crackles and wheezes. Haematological study showed normal haemogram and leukocytosis with neutrophilia. Serum biochemical profile showed hike in total protein and AST levels and fall in albumin levels. However eosinophils, lymphocytes and monocytes values were with in normal range. Thoracic radiography revealed consolidation of lung. Therapeutic efficacy was found to be superior in group II. Combination of Ceftiofur sodium, Flunixin meglumine and Chlorpheneraminemaleate was found to be effective for the treatment of bovine respiratory disease caused by Pasteurella multocida..ThesisItem Open Access STUDIES ON DIAGNOSIS AND THERAPY OF SUBCLINICAL MASTITIS IN BUFFALOES(SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI – 517 502 (A.P.) INDIA, 2016-04) RUPAKALA, S; LAKSHMI RANI, NABSTRACT : On screening of 629 quarters of 158 Graded Murrah buffaloes during the period from November 2014 to October 2015, the quarter-wise and animal-wise prevalence of subclinical mastitis was 28.30 and 40.51 per cent, respectively. The involvement of all the four quarters was more in the buffaloes. The hind quarters were most frequently affected than fore quarters with more prevalence in right hind quarters. There was significant difference (P<0.01) in the prevalence of subclinical mastitis in relation to quarter disposition. The animal-wise prevalence of subclinical mastitis varied significantly (P<0.05) with the lactation number with higher incidence during second lactation. Significant difference (P<0.01) was recorded in the prevalence between different stages of lactation which revealed high prevalence (55.36%) in early lactation followed by mid (44.83%) and late (15.91%) stages of lactation. High prevalence was recorded in buffaloes yielding more than 10 liters/day (58.33%) followed by >5 to 10 liters/day (39.58%) and 2 to 5 liters/day (31.58%). The prevalence based on California Mastitis Test, somatic cell count, electrical resistance and milk pH was 20.34, 21.62, 6.20 and 8.27 per cent, respectively. Bacterial culture was considered to be a gold standard and revealed the prevalence as 28.30 per cent with milk samples of 178 quarters positive for bacterial growth. The CMT reaction was recorded in 67.98 per cent of the culturally positive samples of which 21.35 per cent showed trace reaction while 24.16 (43/178), 17.98 (32/178) and 4.49 per cent (8/178) showed +, ++ and +++ reaction, respectively. The mean ± SE of somatic cell count of milk in healthy control was 98726.56 ± 9508.81 cells/ml and 793707.30 ± 107009.48 cells/ml in quarters positive for subclinical mastitis. Significant difference (P<0.05) was recorded in the mean somatic cell count of healthy and affected milk samples. The mean ± SE of electrical resistance in healthy and subclinical mastitis milk samples was 553.44 ± 18.80 and 477.81 ± 11.26, respectively. Significantly higher pH (P<0.05) was recorded subclinical mastitis positive milk samples (6.89 ± 0.34) while the same in healthy quarters was 6.67 ± 0.02. The bacteriological examination revealed isolation of Staphylococcus spp. (26.40%), followed by Enterococcus spp. (18.54%), Streptococcus spp. (15.17%), Micrococcus spp. (13.48%), Bacillus spp. (12.92%), Klebsiella spp. (11.24%), E. coli (7.30%), Psuedomonas spp. (2.25%) and Salmonella spp. (1.12%) in their order of occurrence. The per cent accuracy of California Mastitis Test, Somatic cell count, electrical resistance and pH were 89.83, 92.69, 77.27 and 79.65, respectively. The antibiotic sensitivity test performed on 30 whole milk samples revealed that 90.00 per cent were sensitive to Moxifloxacin, Amoxycillin/Clavulanic acid and Chloramphenicol, 86.67 per cent were sensitive to Enrofloxacin and Ciprofloxacin, and majority of the isolates (80.00%) were resistant to Penicillin. The bacteriological cure observed on 3rd day was 50.00 per cent vs 60.00 per cent in Group I and II, 61.11 per cent vs 66.67 per cent on 5th day and 66.67 per cent vs 73.33 per cent by 7th day. The overall quarter-wise cure rates were 66.67 per cent and 73.33 per cent, respectively while the animal-wise cure rate was 66.67 per cent in both the groups. The conclusions from the present study were that, subclinical mastitis was a hidden problem in buffaloes and therapy with Moxifloxacin was on par with the efficacy of antibiotics as suggested in the literature, but the use of MnM powder along with Moxifloxacin was found to be superior than antibiotic alone by enhancing udder’s natural defence mechanism, early restoration of milk yield and quick recovery.